Inhibition of DNA repair glycosylases by base analogs and tryptophan pyrolysate, Trp-P-1

• Autorzy: Speina E. , Ciesla J.M. , Graziewicz M.A. , Laval J. , Kazimierczuk Z. , Tudek B.
• Języki publikacji: EN

Abstrakty

EN

DNA base analogs, 2,4,5,6-substituted pyrimidines and 2,6-substituted purines were tested as potential inhibitors of E. coli Fpg protein (formamidopyrimidine -DNA glycosylase). Three of the seventeen compounds tested revealed inhibitory properties. 2-Thioxanthine was the most efficient, inhibiting 50% of 2,6-diamino-4-hydroxy-5N-methyl-formamidopyrimidine (Fapy-7MeG) excision activity at 17.1 μM concentration. The measured Ki was 4.44 ± 0.15 μM. Inhibition was observed only when the Fpg protein was first challenged to its substrate followed by the addition of the base analog, suggesting uncompetitive (catalytic) inhibition. For two other compounds, 2-thio- or 2-oxo-4,5,6-substituted pyrimidines, IC50 was only 343.3 ± 58.6 and 350 ± 24.4 μM, respectively. No change of the Fpg glycosylase activity was detected in the presence of Fapy-7MeG, up to 5 μM.  We also investigated the effect of DNA structure modified by tryptophan pyrolysate (Trp-P-1) on the activity of base excision repair enzymes: Escherichia coli and human DNA glycosylases of oxidized (Fpg, Nth) and alkylated bases (TagA, AlkA, and ANPG), and for bacterial AP endonuclease (Xth protein). Trp-P-1, which changes the secondary DNA structure into non-B, non-Z most efficiently inhibited excision of alkylated bases by the AlkA glycosylase (IC50 = 1 μM). The ANPG, TagA, and Fpg proteins were also inhibited although to a lesser extent (IC50 = 76.5 μM, 96 μM, and 187.5 μM, respectively). Trp-P-1 also inhibited incision of DNA at abasic sites by the α-lyase activity of the Fpg and Nth proteins, and to a lesser extent by the Xth AP endonuclease. Thus, DNA conformation is critical for excision of damaged bases and incision of abasic sites by DNA repair enzymes.

Słowa kluczowe

EN

Fpg protein; base analog; deoxyribose 5-phosphatase; DNA repair glycosylase; 2,4,5,6-substituted pyrimidine; formamidopyrimidine DNA glycosylase; 2,6-substituted purine; 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole; 2,6-diamino-4-hydroxy-5N-methyl-formamidopyrimidine; Escherichia coli; 4,6-diamino-4-hydroxy-5-formamidopyrimidine; DNA repair enzyme; tryptophan pyrolysate; inhibitor

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2005
• Tom: 52
• Numer: 1
• Opis fizyczny: p.167-178,fig.,ref.

Twórcy

autor

Speina E.

• Polish Academy of Sciences, A.Pawinskiego 5A, 02-106 Warsaw, Poland

autor

Ciesla J.M.

autor

Graziewicz M.A.

autor

Laval J.

autor

Kazimierczuk Z.

autor

Tudek B.

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