Inhibition of the helicase activity of HCV NTPase-helicase by 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide-5'-triphosphate [ribavirin-TP]

• Autorzy: Borowski P. , Lang M. , Niebuhr A. , Haag A. , Schmitz H. , Schulze zur Wiesch J. , Choe J. , Siwecka M.A. , Kulikowski T.
• Języki publikacji: EN

Abstrakty

EN

In the presented study the ribavirin-TP — an established inhibitor of the NTPase ac­tivity of the superfamily NTPase/helicases II — was investigated as an inhibitor of the unwinding activity of the hepatitis C virus (HCV) NTPase/helicase. The kinetics of the reaction revealed that ribavirin-TP reduces the turnover number of the helicase reac­tion by a mechanism that does not correspond to that of the inhibition of the NTPase activity. Our results suggest that derivatives of ribavirin-TP with enhanced stability towards hydrolytic attack may be effective inhibitors of the enzyme.

Słowa kluczowe

EN

DNA structure; enzyme inhibition; RNA structure; 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide-5'-triphosphate; NTPase; enzyme; ribavirin-5'-triphosphate; helicase activity; hepatitis C virus

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 3
• Opis fizyczny: p.739-744,fig.

Twórcy

autor

Borowski P.

• Bernhard-Nocht-Institut fur Tropenmedizin, Hamburg, Germany

autor

Lang M.

autor

Niebuhr A.

autor

Haag A.

autor

Schmitz H.

autor

Schulze zur Wiesch J.

autor

Choe J.

autor

Siwecka M.A.

autor

Kulikowski T.

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