EN
Callus tissue culture of Solanum laciniatum Ait. which is a source of diosgenin used in pharmaceutic industry was initiated from leaves and shoots explants on KD solid medium with various combinations of phytohormones. Culture was maintained at 25°C, light intensity 1700 lux and photoperiod 16 h. Obtained callus was treated with plant growth regulators to induce tissue differentiation. Regenerated shoots were rooted on MS medium with half concentration of salts and vitamins and the absence of hormones. Rooted plantlets were planted Into pots with sterilized soil and placed in greenhouse.