The effect of environmental contamination with fluorine on the concentration of adenine nucleotides in the blood of black and white heifers

• Autorzy: Suska M , Lubkowska A.
• Języki publikacji: EN

Abstrakty

EN

The purpose of this study was to examine the influence of environmental fluorine contamination on the adenylate nucleotides (AMP, ADP, AMP), total nucleotide pool (TAN), and adenylate energy charge (AEC) in the erythrocytes of heifers. The concentration of F in serum as well as ATP, ADP, AMP, TAN contents and AEC of red cells were also determined. The animals exposed to F- (group A) came from farms situated about 2-3 km away from the Police chemical plant. The control group involved animals from an ecologically clean area (group B). In comparison with the control, heifers from a study group showed a significant decrease in ATP (p≤0.001) and ADP (p≤0.01), with a parallel increase in erythrocyte AMP (p≤0.001). The means serum F- concentration was higher in exposed animals (6.5 μM, whereas in the control group it was 4.3 μM). The differences were statistically significant (p≤0.05). Both the TAN concentration in serum and the level of AEC in the group of heifers exposed to high F concentration was significantly lower than in the control group (p≤0.001).

Słowa kluczowe

EN

environment contamination; fluorine; adenine nucleotide concentration; blood; heifer; black heifer; white heifer; erythrocyte; control group; organism exposure; animal

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2007
• Tom: 16
• Numer: 3
• Opis fizyczny: p.441-445,fig.,ref.

Twórcy

autor

Suska M

• Szczecin University, al.Piastow 40b, 71-065 Szczecin, Poland

autor

Lubkowska A.

Bibliografia

• 1. JARKOWSKI M., GRABECKI J. Biological monitoring of environmental exposure to fluorine in:. Środowisko i zdrowie. Red. Karski J. B., Pawlak J., Centrum Organizacji i Ekonomiki Ochrony Zdrowia, Warszawa, 1995. [In Polish]
• 2. PAWŁOWSKA-GÓRAL K., WARDAS W., WARDAS M., KUSA Z. The effetcs of fluorides on the human body. Ann. Acad. Med. Siles., 34-35, 105, 1998. [In Polish]
• 3. LONDON R.E., GABEL S. A. Fluorine-19 MNR studies of glucosyl fluoride transport in human erythrocytes. Bioph. J., 69, 1814, 1995.
• 4. MORRIS M., MONTEITH G., RONFOGALIS B. D. The inhibition of ATP-dependent shape change of human erythrocyte ghosts correlates with an inhibition of (Mg⁺²) – ATP activitity by fluoride and aluminofluoride complexes. J. Cell. Biochim., 48, 356, 1982.
• 5. WAKSELM AN C. Fluorinated organic compounds: synthesis and biological applications. Ann. Pharm Fr., 57, 108, 1999.
• 6. YAMAMOTO G., YOSHITAKE K., SATO T., KIMURA T., AANDO T. Distribution and forms of fluorine in whole blood of human male. Anal Bioche., 182, 371, 1989.
• 7. KORKMAZ O. In vitro effects of sodium fluoride and sodium dichromate on dynamic properties of human erythrocyte membrane. Bioph Chem, 83, 111, 2000.
• 8. JÓŹWIAK Z. The participation of adenine nucleotides in the regulation of erythrocyet structure and properties. Post. Hig. Med. Dośw., 35, 116, 1985. [In Polish]
• 9. LEHINGER A.L. Bioenergetics. Warszawa, PWN, 1978. [In Polish]
• 10. ATKINSON D.E. Regulation of enzyme activity. Ann. Rev.Biochem., 35, 85, 1966.
• 11. GUMIŃSKA M. Biochemical mechanisms of fluorine influence on living organisms. Folia Med. Cracov., 23, 305, 1981. [In Polish]
• 12. MACHOY Z. Biochemical mechanisms of fluoride influence on organisms. Folia Med. Cracov., 28, 61, 1987. [In Polish]
• 13. MACHOY-MOKRZYŃSKA A. Fluoride – magnesium interaction. Fluoride, 28 (40), 175, 1995.
• 14. RAĆ M., MACHOY Z., SAFRANOW K. Fluoride as an inhibitor of energy metabolism in a Helix Aspersa Maxima snail limb muscle. In: Metabolizm Fluoru ’02, Eds. Z. Machoy, D. Chlubek, D. Samujło, pp. 37-50, Szczecin 2002. [In Polish]
• 15. SUSKA M., NOWAK R., MACHALIŃSKI B. Serum fluoride and the content of adenine nucleotydes and 2, 3-bisphosphoglycerate in erythrocytes of rats exposed to sodium fluoride. Fluoride, 36 (2), 113, 2003.
• 16. SUSKA M. Energy metabolism of erythrocytes in livestock and experimental animals exposed to fluorides. Szczecin, Uniwersytet Szczeciński, 1985. (doctor thesis, in Polish)
• 17. SUSKA M. The effects of the environemental pollution with fluorides on the concentration of adenine nucleotides in the erythrocytes of Black and White cows. In Polish. Medycyna Wet., 57 (7), 515, 2001. [In Polish]
• 18. JAWOREK D., GRUBER W., BERGMEYER H.U., editors. Methods of Enzymatic Analysis, 2nd ed. New York and London: Verglag Chemie Wienheim and Academic Press. Inc., pp.2127, 1974.
• 19. MARUT A. A simple photocolorymetric method for determining the fluorine concentration in plasma and urine. Diagn Lab. 14, 253, 1978. (In Polish)
• 20. ADAMEK E., PAWŁOWSKA-GÓRAL K., BOBER K. In vitro effects of fluoride ions on enzyme activity. Ann. Acad. Med. Stet., 51, 69, 2005.
• 21. AULAND M.E., MORRIS M.B., ROUFOG ALIS B.D. Separation and characterization of two Mg⁺²-ATPase activities from human erythrocyte membrane. Arch. Biochem. Biophys. 312, 271, 1994.
• 22. BLAYLOCK R.L. Excitoxicity: a possible central mechanism in fluoride neurotoxicity. Fluoride, 37 (4), 301, 2004.
• 23. SCHATZ A., SCHALSCHA E. B., SCHATZ V. The occurrence and importance of paradoxical concentration effects in biological systems. Compost. Science., 5, 22, 1964.
• 24. GUMIŃSKA M., KĘDRYNA T., MARCHUT E., STACHURSKA M.B. ATP, glucose anda lactate in blood of people chronically exposed to fluorine, before and after a preventative application of magnesium salt. Folia Med. Cracov., 26, 93, 1985. (In Polish)
• 25. GUMIŃSKA M. The effects of fluroides on in vitro and in vivo energy homeostasis, and some related biological effects. In: Metabolizm Fluoru ‘94, Eds. Z. Machoy i D.Samujło, 9-12, Szczecin 1994. (In Polish)
• 26. GUMIŃSKA M., SKOWRON-SULA M. The effect of various magnesium and fluoride ions concentrations on in vitro erythrocyte glycolysis. Folia Med. Cracov., 26, 35, 1985. [In Polish]
• 27. HŁYŃCZAK A. J. Energy metabolism, some physical and chemical properties and the structure of erythorcytes in various verterbrates. Biul. WAM, 9, 1, 1970. [In Polish]
• 28. SIKORSKI A., BIAŁKOWSK A K., BISIKIRSKA B., SZOPA J. Erythrocyte and non-erythrocyte spectrin – its structure and functions. Post. Biochem. 39, 50, 1993. [In Polish]