Zastosowanie metody multiplex PCR-RFLP do identyfikacji i roznicowania szczepow enterokokow

• Autorzy: Jarzembowski T.

Warianty tytułu

EN

Application of multiplex PCR-RFLP method [multilocus RFLP] in identification and differentiation of enterococci

• Języki publikacji: PL

Abstrakty

PL

Celem badań było ustalenie czy modyfikacja metody MLST opracowanej przez Sreedhar i wsp. (2002) do różnicowania szczepów enterokoków pozwala na uzyskanie wyników wystarczających do realizacji powyższego zadania. Wprowadzona modyfikacja polegała na zastąpieniu sekwencjonowania produktu amplifikacji analizą restrykcyjną. Wyniki analizy 32 szczepów Enterococcus faecalis izolowanych w regionie gdańskim potwierdziły przydatność powyższej modyfikacji do różnicowania szczepów enterokoków.

EN

The present study proposed use of modified multilocus sequence typing (MLST) approach, for subspecies differentiation of Enterococcus faecalis isolates. We disgesed with Sspl endonuclease product of amplification of three E. faecalis antigen-encoding genes (ace, encoding a collagen and laminin adhesin; efaA, encoding an endocarditis antigen; and salA, encoding a cell wall associated antigen) and one housekeeping gene (pyrC) of 32 E. faecalis isolates MLRA analysis of polymorphic regions of these four genes identified 23 distinct types 21,4 to 100% identity among the 32 isolates. Further studies are needed to evaluate the value of proposed method.

Słowa kluczowe

PL

Enterococcus faecalis; szczepy bakteryjne; metoda RFLP; enterokoki; bakterie; roznicowanie; metoda multiplex PCR; identyfikacja

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2004
• Tom: 56
• Numer: 2
• Opis fizyczny: s.133-137,tab.,rys.,bibliogr.

Twórcy

autor

Jarzembowski T.

• Akademia Medyczna, Gdansk

Bibliografia

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