Ocena interakcji zachodzacych pomiedzy szczepami S. aureus a komorkami fagocytujacymi krwi obwodowej

• Autorzy: Baran J. , Pajdak E. , Isendorf M. , Szmyd D.
• Języki publikacji: PL

Abstrakty

PL

Szczepy S. aureus w zależności od źródła izolacji różnią się opornością na bakteriobójczą aktywność fagocytów - szczepy izolowane z krwi wykazują największą, a szczepy izolowane z dróg oddechowych najmniejszą podatność na zabijanie. Badane szczepy były także zróżnicowane pod względem zdolności do indukcji chemiluminescencji (CL) w monocytach. Nie stwierdzono natomiast różnic w toksyczności badanych drobnoustrojów wobec komórek fagocytujących.

EN

The aim of this study was to investigate the interactions occurring between peripheral blood phagocytes and strains of S. aureus isolated from different clinical specimens (blood, respiratory tract, pus). To evaluate the sensitivity of microorganisms to bactericidal activity of phagocytes, monocytes and granulocytes separated from peripheral blood by standard density gradient and by counter-current centrifugal evaluation these cells were incubated with suspensions of opsonized bacteria. In paralell, the viability of phagocytes was examined by flow cytometry, and the ability of bacteria to trigger reactive oxygen intermediates (ROI) production was evaluated by chemiluminescence measurement. To investigate the efficiency of phagocytosis, bacteria were labelled with fluorescein isothiocynate (FITC) and the percentage of cells containing FITC-labelled bacteria were analysed by flow cytometry. The data obtained show the strains of S. aureus derived from different clinical specimens, differ in their sensitivity to bactericidal activity of phagocytes - strains isolated from the blood show the highest, but strains isolated from the respiratory tract have the lowest sensitivity to killing. These strains differ too in their ability to trigger monocyte CL response. On the contrary, there was no difference in toxicity of bacteria against phagocytes. Strains isolated from peripheral blood showed a significant negative correlation between the ability to trigger CL response and toxicity against phagocytes.

Słowa kluczowe

PL

infekcja bakteryjna; interakcje; szczepy bakteryjne; komorki fagocytarne; krew obwodowa; Staphylococcus aureus

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 1998
• Tom: 50
• Numer: 3-4
• Opis fizyczny: s.131-140,wykr.,bibliogr.

Twórcy

autor

Baran J.

• Uniwersytet Jagiellonski, 30-663 Krakow, ul.Wielicka 238

autor

Pajdak E.

autor

Isendorf M.

autor

Szmyd D.

Bibliografia

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