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1994 | 41 | 4 |

Tytuł artykułu

DNA damage and repair in normal and neoplastic cells treated with adriamycin

Warianty tytułu

Języki publikacji

EN

Abstrakty

Adriamycin (ADR), a common antineoplastic drug, was used to study DNA repair synthesis, cell cytotoxicity and DNA single strand breaks in normal human fibroblasts — CLV98 and human melanoma cells — ME18. No repair synthesis was observed in ME18 and CLV98 cells exposed to adriamycin in concentrations up to 10~5 M. ME18 cells were less sensitive to ADR treatment than CLV98 cells. Adriamycin-induced DNA single strand breaks (at ADR concentration: 1 pg/ml) were incompletely repaired in ME18 cells and unrepaired in CLV98 cells within 24 h after drug removal. Within 48 h strand breaks were completely repaired in both kinds of cells. No repair of single strand breaks was observed in ME18 and CLV98 cells after drug treatment in the concentration of 5 Jig/ml.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

41

Numer

4

Opis fizyczny

p.386-390,fig.

Twórcy

  • Drug Institute, Chelmska 30-34, 00-725 Warsaw, Poland
autor

Bibliografia

  • 1. Evans, DP, Meyn, R.E. & Tomnsovic, S.P. (1986) Survival of rat mammary cell clones and DNA strand damage following adriamycin treat­ment. Cancer Chemother. Pharmacol. 18,137-139.
  • 2. Shimpo, K., Nagatsu, T., Yamada, K., Sato, T., Numi, I IvShamoto, M./Iakeuch^T., Umczawa, H. & Fujita, K. (1991) Ascorbic acid and adria­mycin toxicity. Am. J. Clin. Nulr. 54,1298-1301.
  • 3. Pommicr, Y., Zwelling, L.A., Kao-Shan, Ch.S., Wang-Peng, J. & Bradley, M.O. (1985) Correlations between intercalator-induccd DNA strand breaks and sister chromatid exchanges, mutations and cytotoxicity in Chinese hamster cells. Cancer Res. 45,3143-3149.
  • 4. Lambert, B.,Sten, M.,Soderhall,S., Ringborg, U. & Lewensohn, R. (1983) DNA repair replication, DNA breaks and sister-chromatid exchange in human cells treated with adriamycin in vitro. Mutat. Res. Ill, 171-184.
  • 5. Van-Buul, P.P., Seelan, C.M., Aechoppink, K. & Goudzwaard, J.H. (1992) Absence of translo­cation induction in mouse stem cell sperma­togonia by chemical mutagens probably due to selective elimination. Mutagenesis 7,271-275.
  • 6. Capranico, G., Soranzo, C. & Zunino, F. (1986) Single-Strand DNA breaks induced by chromo- phore-modified anthracyclines in P388 leukemia cells. Cancer Res. 46,5499-5503.
  • 7. Zwelling, L.A., Kerrigan, D. & Michaels, S. (1982) Cytotoxicity and DNA strand breaks by 5-iminodaunorubicin in mouse leukemia LI 210 cells: Comparison with adriamycin and 4'-(9- -acridinylamino) methanesulfon-w-anisidine. Cancer Res. 42,2687-2691.
  • 8. Ross, W.E. & Smith, M.C. (1982) Repair of deoxyribonucleic acid lesions caused by adriamycin and ellipticine. Diochem. Pharmacol. 31,1931-1935.
  • 9. Mosman, T. (1983) Rapid colorimotric assay for cellular growth and survival: Application to proliferation and cytotoxicity assay. /. Immunol. Methods 65,55-63.
  • 10. Trosko, J.E. & Yager, J.D. (1974) A sensitive method to measure physical and chemical carcinogen-induced "unscheduled DNA synthesis" in rapidly dividing eucaryotic cells. Exp. Cell Res. 88,47-55.
  • 11. Anuszewska, E. & Koziorowska, J. (1985) DNA repair and sensitivity of mouse embryo fibro­blasts to methyl methanesulphonate and N-me- thyl-N'-nitro-N-nitroso-guanidine. Acta Bio- chim. Polon. 32,295-304.
  • 12. Kohn, K.W. (1987) DNA filter elution methods in anticancer drug development; in Concepts, clinical developments and therapeutic advances in cancer chemotherapy (Muggia, F.M., ed.) Marti- nus Nijhoff Publishers, Boston.
  • 13. Kohn, K.W. (1991) Principles and practice of DNA filter elution. Pharmacol. Ther. 49,55-77.
  • 14. Brunborg, J. Holme, J.A., Soderlung, E.J. Omichinski, J.G. & Dybing, E. (1988) An automated alkaline elution system: DNA damage induccd by l,2-dibromo-3-chloro- propane in vivo and in vitro. Anal. Biochem. 174, 522-536.
  • 15. Brunborg, G., Holme, J.A., Soderlund, E.J. & Dybing, E. (1990) Organ-specific genotoxic effects of chemicals: The use of alkaline elution to detcct DNA damage in various organs of in two-exposed animals; in Mutation and the Environment, Part D, pp. 43-52, Wiley-Liss, Inc.
  • 16. Twentyman, PR. & Luscombe, M. (1987) A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity. Br. J. Cancer 56, 279-285.
  • 17. Sargent, J.M. & Taylor, C.G. (1989) Appraisal of the MTT assay as a rapid test of chemosensi­tivity in acute myeloid leukaemia. Br. J. Cancer 60,206-210.
  • 18. Wilson, J.K., Sargent, J.M., Elgie, A.W., Hill, J.G. & Taylor, C.G. (1990) A feasibility study of the MTT assay for chemosensitivity testing in ovarian malignancy. Br. J. Cancer 62,189-194.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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