Microarray analysis reveals the role of matrix metalloproteinases in mouse experimental autoimmune myocarditis induced by cardiac myosin peptides

• Autorzy: Tang Q , Huang J. , Qian H. , Xiong R. , Shen D. , Wu H. , Bian Z. , Wei X.
• Języki publikacji: EN

Abstrakty

EN

Autoimmune myocarditis develops after the presentation of heart-specific antigens to autoaggressive CD4+ T cells and after inflammation has infiltrated the tissues. To shed light on global changes in the gene expression of autoimmune myocarditis and to gain further insight into the molecular mechanisms underlying the genesis of myocarditis, we conducted a comprehensive microarray analysis of mRNA using an experimental mouse autoimmune myocarditis model via immunization with α-myosin heavy chain-derived peptides. Of over 39,000 transcripts on a high density oligonucleotide microarray, 466 were under-expressed and 241 over-expressed by ≥ 1.5-fold compared with the controls in BALB/C mouse with autoimmune myocarditis. In this paper, we list the top 50 up-regulated genes related to the immune response. These altered genes encode for leukocyte-specific markers and receptors, the histocompatibility complex, cytokines/receptors, chemokines/receptors, adhesion molecules, components of the complement cascade, and signal transduction-related molecules. Interestingly, matrix metalloproteinases (MMPs) such as MMP-3 and MMP-9 were up-regulated, as further revealed by the reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry assays. This indicates that MMPs may act as major regulators of the cytokine profile. Together, these findings provide new insight into the molecular events associated with the mechanism of the autoimmune genesis of myocarditis.

Słowa kluczowe

EN

myocarditis; pathogenesis; mouse; mice; gene expression; matrix metalloproteinase; microarray analysis; autoimmune myocarditis; cardiac failure; alpha-myosin heavy chain-derived peptide; cardiac myosin peptide; young adult

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2007
• Tom: 12
• Numer: 2
• Opis fizyczny: p.176-191,fig.,ref.

Twórcy

autor

Tang Q

• Wuhan University, 430060 Wuhan, P.R.China

autor

Huang J.

autor

Qian H.

autor

Xiong R.

autor

Shen D.

autor

Wu H.

autor

Bian Z.

autor

Wei X.

Bibliografia

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