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1993 | 40 | 4 |
Tytuł artykułu

Homology of genes for exopolysaccharide synthesis in Rhizobium leguminosarum and effect of cloned exo genes on nodule formation

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
A 5.4 kb BamHl fragment of R. leguminosarum bv. trifolii TA1 was found to carry genes involved in exopolysaccharide synthesis (caro genes). This fragment was strongly hybridized to the total DNA from R. I. bv. viciae and bv. phaseoli digested with EcoRI. No homology was found with total DNA of R. meliloti and Rhizobium sp. NGR 234. The exo genes from R. I. bv. trifoliiTAl conjugally introduced into R. I. bv. viciae 1302 considerably affected the symbiosis: the nodules induced on vetch were abortive and did not fix nitrogen. On the other hand, Phaseolus beans infected with R. I. bv. phaseoli harbouring R. I. bv. trifolii exo genes formed the nitrogen-fixing nodules. It can be concluded that additional copies of exo genes introduced into wild type Rhizobium leguminosarum strains can disturb the synthesis of acidic exo- polysaccharides and affect symbiosis of the plants forming indeterminate nodules, but do not affect symbiosis of the plants forming the determinate nodules.
Wydawca
-
Rocznik
Tom
40
Numer
4
Opis fizyczny
p.477-482,fig.
Twórcy
autor
  • M.Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland
autor
Bibliografia
  • 1. Chen, H., Batley, M., Redmond, J. & Rolfe, B.G. (1985) Alteration of the effective nodulation properties of a fast-growing broad host range Rhizobium. J. Plant. Physiol. 120,331 - 349.
  • 2. Derylo, M., Skorupska, A., Bcdnara, J. & Lorkiewicz, Z. (1986) Rhizobium trifóli mutants deficient in exopolysaccharide production. Physiol. Plant. 66,699 -7(M.
  • 3. Finan, T.M., Kunkel, B., DeVos, G.F. & Signer, E.R. (1986) Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysacchride and thiamine synthesis genes. J. Bacteriol. 167,66 -72.
  • 4. Chen, H., Gray, J.X., Nayudu, M., Djordjevic, M.A., Batley, M., Redmond, J. & Rolfe, B.C. (1988) Five genetic loci involved in the synthesis of acidic exopolysaccharide are closely linked in the genome of Rhizobium sp. strain NGR 234. Mol. Gen. Genet. 212,310 - 316.
  • 5. Long, S., Reed, J.W., Himawan, G.C. & Walker, G.C. (1988) Genetic analysis of a cluster of genes required for the synthesis of the calcofluor- -binding exopolysaccharide of Rhizobium meli­loti. ]. Bacteriol. 170,4239 - 4248.
  • 6. Gray, J.X., Djordjevic, B.G. & Rolfe, B.G. (1990) Two genes that regulate exopolysaccharide production in Rhizobium sp. strain NGR 234: DNA sequences and resultant phenotypes. /. Bacteriol. 172,193 - 203.
  • 7. Borthakur, D., Downie,J.A.,Johnston, A.W.B. & Lamb, J.W. (1985) Psi, a plasmid-linked Rhizobium phaseoli gene that inhibits exopoly­saccharide production on which is required for symbiotic nitrogen fixation. Mol. Gen. Genet. 200,278 - 282.
  • 8. Borthakur, D., Barbur, C.E., Lamb,J.W., Daniels, M.J., Downie, J.A. & Johnston, A.W.B. (1986) A mutation that blocks exopolysaccharide synthe­sis prevents nodulation of peas by Rhizobium leguminosarum but not of beans by Rhizobium phaseoli and is corrected by cloned DNA from Rhizobium or the phytopathogen Xantomonas. Mol. Gen. Genet. 203,320 - 323.
  • 9. Borthakur, D., Barker, R.F., Larchford, J.W., Rossen, L. & Johston, A.W.B. (1988) Analysis of pss of Rhizobium leguminosarum required for exopolysaccharide synthesis and nodulation of peas: their primary structure and their interaction with psi and other nodulation genes. Mol. Gen. Genet. 213,155-162.
  • 10. Aman, P., McNeil, L., Framen, A.G., Darvill, P. & Albersheim, P. (1981) Structural elucidation, using HPLC-MS, of the acidic polysaccharide secreted by R. meliloti strain 1021. Carbohydr. Res. 95,263-282.
  • 11. Djordjevic, S.P., Rolfe, B.G., Batley, M. & Redmond, J.W. (1986) The structure of the exopolysaccharide from Rhizobium sp. strain ANU 280 (NGR 234). Carbohydr. Res. 148,87 - 89.
  • 12. McNeil, M., Darvill, J., Darvill, A.G., Albe­rsheim, P., VanVeen, R., Hooykaas, P., Schilperoort, R. & Dell, A. (1986) The discernible, structural features of the acidic polysaccharides secreted by different Rhizobium species are the same. Carbohydr. Res. 146, 307 - 326.
  • 13. Hollingsworth, R.I., Dazzo, F.B., Hallega, K. & Musselman, B. (1980) The complete structure of the trifoliin A lectin-binding capsular poly­saccharide of Rhizobium trifolii 843. Carbohydr. Res. 172,97- 112.
  • 14. Canter-Cremers, H., Batley, M., Redmond, J.W., Wijfjes, A.H.M., Lugtenberg, B.J.J. & Wijffelman, C.A. (1991) Distribution of O-acetyl groups in the exopolysaccharide synthesized by Rhizo­bium legumiiwsarum strains is not determined by the Sym plasmid. J. Biol. Chem. 266,9556 - 9564.
  • 15. Janson, P.E., Kenne, L., Lindberg, B., Ljunggren, H., Lonngren, J., Ruden, U. &Svensson,S. (1977) ¡Demonstration of an octasaecharide repeating unit in the extracellular polysaccharide of Rhizobium meliloti by sequential degradation. /. Am. Chem. Soc. 99,3812 - 3815.
  • 16. Leigh, J.A., Reed, J. W., Hanks, J.F., Hirsch,A.M. & Walker, G.C. (1987) Rinzobium meliloti mutants that fail to succinylate their Calcofluor- binding exopolysaccharide are defective in nodule invasion. Cell 51,579 - 587.
  • 17. Leigh, J.A. & Lec, C.C. (1988) Characterization of polysaccharides of Rhizobium meliloti exo mutants that form ineffective nodules. J. Bacteriol. 170,3327 - 3332.
  • 18. Zhan, H., Gray, J.X., Levery, S B., Rolfe, B.G. & Leigh, J.A. (1990) Functional and evolutionary relatedness of genes for exopolysaccharide synthesis in Rhizobium meliloti and Rhizobium sp. strain NGR 234. /. Bacteriol. 172,5245 - 5253.
  • 19. Zhan, H. & Leigh, J.A. (1990) Two genes that regulate exopolysaccharide production in Rhizobium meliloti. J. Bacteriol. 172,5254 - 5259.
  • 20. Doherty, D.J., Leigh, J.A., Glazebrook, J. & Walker, G.C. (1988) Rhizobium meliloti mutants that overproduce the R. meliloti acidic Calco- fluor-binding exopolysaccharide. J. Bacteriol. 170,4249 - 4256.
  • 21. Skorupska, A., Derylo, M. & Golinowski, W. (1991) The region for exopolysaccharide syn­thesis in Rhizobium leguminosarum biovar trifolii is located on the non-symbiotic plasmid. Acta. Biochim. Polon. 38,423 - 435.
  • 22. Urbanik-Sypniewska, T., Skorupska, A. & Derylo, M. (1992) Exopolysaccharides of Rhizobium leguminosarum biovar trifolii harbouring cloned exo region. Acta Biochim. Polon. 39,1-15.
  • 23 Kowalski, M. (1967) Transduction in Rhizobium meliloti. Acta Microbiol. Polon. 16,7-10.
  • 24. Wijffelman, C.A., Pecs, E., Van Brussel, A.A.N. & Hooykaas, P.J.J. (1983) Repression of small bacteriocin excretion in Rhizobium legumi- nosarum and Rhizobium trifolii by transmissible plasmids. Mol. Gen. Getiet. 192,171 -176.
  • 25. Staniewski, R. (1970) Typing of Rhizobium by phages. Can. J. Microbiol. 16,1003 -1009.
  • 26. Figurski, D.H. & Helinski, D.R. (1979) Repli­cation of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Prvc. Natl. Acad. Sci. U.S.A. 76, 1648-1652.
  • 27. Vincent, J.M. (1980) Factors controlling the Rhizobium - legume symbiosis; in Nitrogen Fixation (Newton, W.E. & Ormc-Johnston, W.H., eds.) vol. 2, University Park Press, Baltimore.
  • 28. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press.
  • 29. Hotter, G.S. & Scott, D.B. (1991) Exopolysa­ccharide mutants of Rhizobium loti are fully effective on a determinate nodulating host but are ineffective on an indeterminate nodulating host. /. Bacteriol. 173,851 - 859.
Typ dokumentu
Bibliografia
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bwmeta1.element.agro-article-f593ca3c-4b05-483b-8837-398670239f1e
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