Desulfovibrio desulfuricans lipopolysaccharides induce endothelial cell IL-6 and IL-8 secretion and E-selectin and VCAM-1 expression

• Autorzy: Weglarz L , Dzierzewicz Z. , Skop B. , Orchel A. , Parfiniewicz B. , Wisniowska B. , Swiatkowska L. , Wilczok T.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to determine whether Desulfovibrio desulfuricans-derived LPS stimulate the release of IL-6 and IL-8 from ECs and the expression of their adhesion molecules at the transcriptional level. Confluent monolayers of HUVEC were incubated in the absence or presence of 20 μg/ml and 60 μg/ml LPSs derived from the DdT and DdA bacterial strains. Also, the simultaneous stimulation of cells with LPSs and IL-1β was evaluated. The levels of cytokines released were measured using ELISA. LPS-activated HUVEC increased the secretion of both IL-6 and IL-8, which was not LPS dose dependent. The expression of E-selectin and VCAM-1 was assessed by TR-PCR. The transcripts were detectable at all the concentrations (20, 40, 60 μg/ml) of LPSs used. These results suggest that D. desulfuricans LPS may activate immune functions in endothelial cells and influence the inflammatory response during bacteremia caused by these bacteria.

Słowa kluczowe

EN

interleukin 8; adhesion molecule; interleukin 6; cell adhesion molecule; lipopolysaccharide; cytokine; endothelial cell; ELISA test; polymerase chain reaction; Desulfovibrio desulfuricans

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2003
• Tom: 08
• Numer: 4
• Opis fizyczny: p.991-1003,fig.

Twórcy

autor

Weglarz L

• Medical University of Silesia, Narcyzow 1, 41-200 Sosnowiec, Poland

autor

Dzierzewicz Z.

autor

Skop B.

autor

Orchel A.

autor

Parfiniewicz B.

autor

Wisniowska B.

autor

Swiatkowska L.

autor

Wilczok T.

Bibliografia

• 1. Pober, J.S. and Cotran, R.S. Cytokines and endothelial cell biology. Physiol. Rev. 70 (1990) 427-451.
• 2. Pober, J.S. and Cotran, R.S. The role of endothelial cells in inflammation. Transplantation 50 (1990) 537-541.
• 3. Mantovani, A., Bussolino, F. and Introna, M. Cytokine regulation of endothelial cell function. From molecular level to the bedside. Immunol. Today 18 (1997) 231-240.
• 4. Chen, C.C. and Manning, A.M. TGF-β1, IL-10 and IL-4 differentially modulate the cytokine-induced expression of IL-6 and IL-8 in human endothelial cells. Cytokine 8 (1996) 58-65.
• 5. Bevilacqua, M.P. Endothelial-leukocyte adhesion molecules. Annu. Rev. Immunol, 11 (1993) 767-804.
• 6. Haraldsen, G., Kvale, D., Lien, B., Farstad, I.N. and Brandtzaeg, P. Cytokine-regulated expression of E-selectin, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) in human intestinal microvascular endothelial cells. J. Immunol. 156 (1996) 2558-2565.
• 7. Jehle, A.B., Li, Y., Stechschulte, A.C., Stechschulte, D.J. and Dileepan, K. Endotoxin and mast cell granule proteases synergistically activate human coronary artery endothelial cells to generate interleukin-6 and interleukin-8. J. Interferon Cytokine Res. 20 (2000) 361-368.
• 8. von Asmuth, E.J., Leeuwenberg, J.F., Ceska, M. and Buurman, W.A. LPS and cytokine-induced endothelial cell IL-6 release and ELAM-1 expression; involvement of serum. Eur. Cytokine Netw. 2 (1991) 291-297.
• 9. Charreau, B., Coupel, S., Goret, F., Pourcel, C. and Soulillou, J.-P. Association of glucococorticoids and cyclosporin A or rapamycin prevents E-selectin and IL-8 expression during LPS- and TNFα-mediated endothelial cell activation. Transplantation 69 (2000) 945-953.
• 10. Dofferhoff, A.S., Nijland, J.H., deVries-Hospers, H.G., Mulder, P.O., Weits, J. and Bom, V.J. Effects of different types and combinations of antimicrobial agents on endotoxin release from gram-negative bacteria: an in vitro and in vivo study. Scand. J. Infect. Dis. 23 (1991) 745-754.
• 11. Evans, M.E. and Pollack, M. Effect of antibiotic class and concentration on the release of lipopolysaccharide from Escherichia coli. J. Infect. Dis. 167 (1993) 1336-1343.
• 12. Mayeux, P. R. Pathobiology of lipopolysaccharide. J. Toxicol. Env. Health. 51 (1997) 415-435.
• 13. Fox, J.G., Dewhirst, F.E., Fraser, G.J., Shames, B. and Murphy, J.C. Intracellular Campylobacter-like organism from ferrets and hamsters with proliferative bowel disease is a Desulfovibrio sp. J. Clin. Microbiol. 32 (1993) 1229-1237.
• 14. Dzierżewicz, Z., Cwalina, B., Kurkiewicz, S., Chodurek, E. and Wilczok, T. Intraspecies variability of cellular fatty acids among soil and intestinal strains of Desulfovibrio desulfuricans. Appl. Environ. Microbiol. 62 (1996) 3360-3365.
• 15. Dzierżewicz, Z., Cwalina, B., Gawlik, B., Wilczok, T. and Gonciarz, Z. Isolation and evaluation of susceptibility to sulphasalazine of Desulfovibrio desulfuricans strains from the human digestive tract. Acta Microbiol. Pol. 46 (1997) 175-187.
• 16. Gibson, G.R., Macfarlane, G.T. and Cummings, J.H. Occurence of sulphate- reducing bacteria in human faeces and the relationship of dissimilatory sulphate reduction to methanogenesis in the large gut. J. Appl. Bacterial. 65 (1988) 103-111.
• 17. Dzierżewicz, Z., Gawlik, B., Cwalina, B., Gonciarz, K., Ziółkowski, G., Gonciarz, Z. and Wilczok, T. Activity of sulphate reducing bacteria in the human digestion tract. Bull Pol. Acad. Sci. Biol. 42 (1994) 171-176.
• 18. Florin, T.H.J., Gibson, G.R., Neale, G. and Cummings, J.H. A role for sulphate-reducing bacteria in ulcerative colitis? Gastroenterology 98A (1990) 170.
• 19. Gibson, G.R., Cummings, J.H. and MacFarlane G.T. Growth and activities of sulphate-reducing bacteria in gut contents of healthy subjects and patients with ulcerative colitis. FEMS Microbiol. Ecol. 86 (1991) 103-112.
• 20. Schoenborn, L., Abdollahi, H., Tee, W., Dyall-Smith, M. and Janssen, P.H. A member of the delta subgroup of proteobacteria from a pyogenic liver abscess is a typical sulphate reducer of the genus Desulfovibrio. J. Clin. Microbiol. 39 (2001) 787-790.
• 21. Tee, W., Dyall-Smith, M., Woods, W. and Eisen, D. Probable new species of Desulfovibrio isolated from a pyogenic liver abscess. J. Clin. Microbiol. 34 (1996) 1760-1764.
• 22. Lozniewski, A., Mauer, P., Schuhmacher, H., Carlier, J.P. and Mory, F. First isolation of Desulfovibrio sp. as part of a polymicrobial infection from a brain abscess. Eur. J. Clin. Microbiol. Infect. Dis. 18 (1999) 602-603.
• 23. Shukla, S. and Reed, K.D. Desulfovibrio desulfuricans bacteremia in a dog. J. Clin. Microbiol. 38 (2000) 1701-1702.
• 24. Loubinoux, J., Mory, F., Pereira, I.A.C. and Le Faou, A.E. Bacteremia caused by a strain of Desulfovibrio related to the provisionally named Desulfovibrio fairfieldensis. J. Clin. Microbiol. 38 (2000) 931-934.
• 25. Porsche, R. K. and Chan, P. Anaerobic vibrio-like organisms cultured from blood: Desulfovibrio desulfuricans and Succinivibrio species. J. Clin. Microbiol. 5 (1997) 444-447.
• 26. Baron, E.J., Bennion, R., Thompson, J., Strong, C., Summanen, P., McTeague, M. and Finegold, S.M. A microbiological comparison between acute and complicated appendicitis. Clin. Infect. Dis. 14 (1992) 227-231.
• 27. Węglarz, L., Dzierżewicz, Z., Orchel, A., Szczerba, J., Jaworska-Kik, M. and Wilczok, T. Biological activity of Desulfovibrio desulfuricans lipopolysaccharides evaluated via interleukin-8 secretion by Caco-2 cells. Scand. J. Gastroenterol. 38 (2003) 73-79.
• 28. Jaffe, E.A., Nachman, R.L., Becker, C.G. and Minek, C.R. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J. Clin. Invest. 52 (1973) 2745-2755.
• 29. Dzierżewicz, Z., Cwalina, B., Węglarz, L. and Głąb, S. Isolation and evaluation of corrosive aggressivity of wild strains of sulphate-reducing bacteria. Acta Microbiol. Pol. 42 (1992) 211-221.
• 30. Postgate, J.R. The Sulphate-Reducing Bacteria. Cambridge: Cambridge University Press, 1984.
• 31. Johnson, K.G. and Perry, M.B. Improved technique for the preparation of bacterial lipopolysaccharides. Can. J. Microbiol. 22 (1975) 29-34.
• 32. Lawden, K.H., Pitts, J.M., Thomas, J.A. and Lowe, C.R. Rational computer-aided design of ligands that bind endotoxin, in: Bacterial Endotoxins: Lipopolysaccharides from Genes to Therapy. (Levin, J., Alving, C.R., Munford, R.S. and Redl, H., Eds.), Wiles-Liss, Inc., 1995, 443-452.
• 33. Masamune, A., Shimosegawa, T., Kimura, K., Fujita, M., Sato, A., Koizumi, M. and Toyota, T. Specific induction of adhesion molecules in human vascular endothelial cells by rat experimental pancreatitis-associated ascitic fluids. Pancreas 18 (1999) 141-150.
• 34. Zhang, H., Niesel, D.W., Peterson, J.W. and Klimpel, G.R. Lipoprotein release by bacteria: Potential factor in bacterial pathogenesis. Infect. Immun. 66 (1998) 5196-5201.
• 35. Leeson, M.C.Y. and Morrison, D.C. Induction of proinflammatory responses in human monocytes by particulate and soluble forms of LPS. Shock 2 (1994) 235-245.
• 36. Dinarello, C.A. Cytokines as mediators in the pathogenesis of septic shock. Curr. Top. Microbiol. Immunol. 216 (1996) 133-165.
• 37. Panes, J. and Granger, D.N. Leukocyte-endothelial cell interactions: Molecular mechanisms and implications in gastrointestinal disease. Gastroenterology 114 (1998) 1066-1090.
• 38. Fries, J.W., Williams, A.J., Atkins, R.C., Newman, W., Lipscomb, M.F. and Collins, T. Expression of VCAM-1 and E-selectin in an in vivo model of endothelial activation. Am. J. Pathol. 143 (1993) 725-737.
• 39. Swerlick, R.A., Lee, K.H., Sepp, N.T., Caughman, S.W. and Lawley, T.J. Regulation of vascular cell adhesion molecule 1 on human dermal microvascular endothelial cells. J. Immunol. 149 (1992) 689-705.