Effect of cytokinins on in vitro morphogenesis and ploidy of pepper Capsicum annuum L.

• Autorzy: Tomaszewska-Sowa M. , Drozdowska L. , Szota M.
• Języki publikacji: EN

Abstrakty

EN

The study-compared effect of cytokinins on morphogenesis and ploidy of plants regenerated in vitro from explants of ‘Stanola F1’, ATZ and ATM pepper seeds. The aim of defining the morphogenetic potential of the studied genotypes, half-seed explants consisting the proximal part of the hypocotyl and radicle were put onto the MS medium containing BAP (5.0 mg.dm-3), 2iP (2.5 mg.dm-3), ZEA (2.5 mg.dm-3), TDZ (1.5 mg.dm-3), while MS medium without cytokinins constituted the control. After the initiation period, explants were transferred onto the medium without cytokinins. The effect of the growth regulators to the morphogenetic response of explants was estimated based on the number of explants on which adventitious buds and shoots were developing. The cytokinins applied did not show a significant effect on the development of adventitious buds on pepper explants.

Słowa kluczowe

EN

ploidy; plant physiology; Capsicum annuum; pepper; cytokinin; in vitro; morphogenesis

• Wydawca: -
• Czasopismo: Electronic Journal of Polish Agricultural Universities. Series Agronomy
• Rocznik: 2002
• Tom: 05
• Numer: 1
• Opis fizyczny: http://www.ejpau.media.pl/series/volume5/issue1/agronomy/art-04.html

Twórcy

autor

Tomaszewska-Sowa M.

• University of Technology and Agriculture, Bernardynska 6, 85-029 Bydgoszcz, Poland

autor

Drozdowska L.

autor

Szota M.

Bibliografia

• Arroyo R., Revilla M.A. 1991. In vitro plant regeneration from cotyledon and hypocotyl segments in two bell pepper cultivars. Plant Cell Rep. 10, 414–416.
• Auer C.A., Cohen J.D., Laloue M., Cooke T.J., 1992. Comparison of benzyl adenine metabolism in two Petunia hybrid lines differing in shoot organogenesis. Plant Physiol. 98, 1035–1041.
• Binzel M. L., Sankhla N., Joshi S., Sankhla D., 1996. In vitro regeneration in chilli pepper (Capsicum annuum L.) from half-seed explants. Plant Growth Regulation 20, 287–293.
• Borychowski A., Niemirowicz-Szczytt K., 1997. Wybrane czynniki wpływające na regenerację in vitro pędów z hypokotyli papryki (Capsicum annuum L.) [Selected factors affecting the regeneration in vitro of shoots from pepper hypocotyls (Capsicum annuum L.)]. Zesz. Nauk. AR w Krakowie, Sesja Nauk. 50, 161–166 [in Polish].
• Bretagne B., Chupeau M. – C., Chupeau Y., Fouilloux G., 1994. Improved flax regeneration from hypocotyls using thidiazuron as a cytokinin source. Plant Cell Rep. 14, 120–124.
• Christopher T., Rajam M.V., 1994. In vitro clonal propagation of Capsicum spp. Plant Cell Tiss. Org. Cult. 38, 25–29.
• Christopher T., Rajam M.V., 1996. Effect of genotype, explant and medium on in vitro regeneration of red pepper. Plant Cell Tiss. Org. Cult. 46, 245–250.
• Ebida A.I.A., Hu C.Y., 1993. In vitro morphogenetic responses and plant regeneration from pepper (Capsicum annuum L. cv. Early California Wonder) seedling explants. Plant Cell Rep. 13, 107-110.
• Ezura H., Nishimiya S., Kasumi M., 1993. Efficient regeneration of plants independent of exogenous growth regulators in bell pepper (Capsicum annuum L.). Plant Cell Rep. 12, 287–293.
• Fari M., Andrasfalvy A., 1994. Regeneration and cloning of pepper (Capsicum sp.) in vitro: a review. HortScience 26 (2), 9-18.
• Galoch E., Buracka – Łaukajtys E., Kopcewicz J., 1996. Effect of cytokinins on flower differentiation in cultured plantlets of Pharbitis nil Chois. Acta Physiol. Plant. 18 (3), 223–227.
• Gatz A., Rogozińska J., 1994. In vitro organogenetic potential of cotyledon and leaf explants of Capsicum annuum L. cv. Bryza. Acta Soc. Bot. Pol. 63 (3-4), 255–258.
• Huettemann C.A., Preece J.E., 1993. Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Tissue Org. Cult. 33, 105–119.
• Kinet J.-M., 1993. Environmental, chemical and genetic control of flowering. Hort. Rev. 15, 279–334.
• Manoharan M., Sree Vidya C. S., Lakshmi Sita G., 1998. Agrobacterium – mediated genetic transformation in hot chili (Capsicum annuum L. var. Pusa jwala). Plant Sci. 131, 77–83.
• Morrison R.A., Konning R.E., Evans D.A., 1986. Pepper. In: Handbook of Plant Cell Culture. Eds. D.A. Evans, W.R. Sharp, V.A. Philip. Vol. 4, 552–573. New York, Macmillan.
• Murashige T., Skoog F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.
• Orlikowska T., 1997. Regulatory roślinne w kulturach in vitro. W: Regulatory wzrostu i rozwoju roślin [Plant regulators in cultures in vitro. In: Plant growth and development regulators]. Red. L. S. Jankiewicz. PWN Warszawa [in Polish].
• Ramage C.M., Leung D.W.M., 1996. Influence of BA and sucrose on the competence and determination of pepper (Capsicum annuum L. var. Sweet Banana) hypocotyl cultures during shoot formation. Plant Cell Rep. 15, 974–979.
• Ramirez-Malagon R., Ochoa-Alejo N., 1996. An improved and reliable chilli pepper (Capsicum annuum L.) plant regeneration method. Plant Cell Rep. 16, 226–231.
• Rogozińska J., Drozdowska L., 1996. In vitro culture of bell pepper (Capsicum annuum cv. Bryza) and its greenhouse performance. J. Appl. Genet. 37 (4), 357–366.
• Rogozińska J., Drozdowska L., 1997. Tworzenie kwiatów i owoców papryki w kulturach in vitro [Sweet pepper flower and fruit formation in cultures in vitro]. Ogrodnictwo 5, 17–19 [in Polish].
• Rogozińska J., Tobolewska G., 1992. Genotypic variations in organogenesis of six cultivars of pepper, Capsicum annuum L. Gen. Pol. 33, 213–217.
• Saxena P. K., Gill R, Radashid A., Mehashwari S.C., 1981. Isolation and culture of protoplast of Capsicum annuum L. and their regeneration into plants flowering in vitro. Protoplasma 108, 357–360.
• Scorza R., Janick J., 1980. In vitro flowering of Passiflora suberosa L. J. Amer. Soc. Hort. Sci. 105 (6), 892–897.
• Szasz A., Nervo G., Fari M., 1995. Screening for in vitro shoot-forming capacity of seedling explants in bell pepper (Capsicum annuum L.) genotypes and efficient plant regeneration using thidiazuron. Plant Cell Rep. 14, 666–669.
• Valero-Montero L.L., Ochoa-Alejo N., 1992. A novel approach for chilli pepper (Capsicum annuum L.) plant regeneration: Shoot induction in rooted hypocotyls. Plant Sci. 84, 215–219.
• Zhu Y.X., Ou – Yang W.J., Zhang Y.F., Chen Z.L., 1996. Transgenic sweet pepper plants from Agrobacterium mediated transformation. Plant Cell Rep. 16, 71–75.