Competitive inhibitors of free and chitosan-immobilized urease

• Autorzy: Zaborska W.
• Języki publikacji: EN

Abstrakty

EN

The influence of four inhibitors: boric acid, thioglycolic acid, sodium fluoride and acetohydroxamic acid on the activity of urease, both in the native form and immo­bilized covalently on glutaraldehyde-pretTeated chitosan membrane, was studied. Urea hydrolysis was carried out in phosphate buffer, pH 7, at 25°C at urea concen­tration of 50 mM. The immobilized urease was more resistant than the native one to the action of all the investigated inhibitors, except boric acid. This property of the enzyme offers a possibility of its practical application.

Słowa kluczowe

EN

urease inhibitor; competitive inhibitor; thioglycolic acid; urease immobilization; chitosan membrane; acetohydroxamic acid; boric acid; sodium fluoride

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 1
• Opis fizyczny: p.115-118,fig.

Twórcy

autor

Zaborska W.

• Jagiellonian University, R.Ingardena 3, 30-060 Cracow, Poland

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