Evaluation of the developmental toxicity of gentamicin using postimplantation rat whole embryo culture

• Autorzy: Biernacki B , Wlodarczyk B. , Minta M.
• Języki publikacji: EN

Abstrakty

EN

The embryotoxicity of gentamicin was evaluated with rat whole embryo culture (WEC) assay. Rat embryos displaying 1-3 somites were explanted on gestation day (GD) 9.5 and cultured for 48 h in rat serum. Gentamicin sulfate was added to the culture medium at a concentration of 1, 10 and 100 μg/ml. At the end of the cultivation five endpoints were evaluated for each viable embryo: yolk-sac diameter, crown-rump length, head length, somites number and morphological score. Gentamicin at the concentration of 100 μg/ml was embryotoxic and caused growth retardation in rat embryos. At the concentration of 10 μg/ml about 45% of embryos showed haematomas. The lowest dose of antibiotic (1 μg/ml) did not interfere with embryonic development in vitro.

Słowa kluczowe

EN

developmental toxicity; gentamicin; toxicity; embryo culture; toxicology; embryotoxicity; rat

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2001
• Tom: 45
• Numer: 2
• Opis fizyczny: p.347-352,fig.

Twórcy

autor

Biernacki B

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Wlodarczyk B.

autor

Minta M.

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