Ozone- and ethylene-induced regulation of a grapevine resveratrol synthase promoter in transgenic tobacco

• Autorzy: Grimmig B , Schubert R. , Fischer R. , Hain R. , Schreier P.H. , Betz C. , Langebartels C. , Ernst D. , Sandermann H.
• Języki publikacji: EN

Abstrakty

EN

Stilbene synthases (STSs) are enzymes that play a critical role in the biosynthesis of stilbene, phytoalexins in a small number of unrelated plant species, and are induced by various biotic and abiotic stressors like pathogen attack, UV-irradiation or ozone exposure. To investigate the molecular basis for ozone-induced plant stress responses, we have examined the promoter of the grapevine resveratrol synthase (Vst1). In this report we summarize the influence of ozone on gene regulation. In transgenic tobacco a chimeric gene construct, containing the Vst1 promoter combined with the β-glucuronidase (GUS) reporter gene, is rapidly induced by ozone (0.1 µl·l⁻¹, 12 h). The same construct is also strongly induced by ethylene (20 µl·l⁻¹, 12 h). Promoter deletion analysis of the 5′ flanking sequence identified a positive regulatory element between −430 bp and −280 bp. This region contains ethylene-responsive enhancer elements, as well as an elicitor-responsive sequence in inverse orientation.

Słowa kluczowe

EN

gene regulation; promoter; ozone; pathogen; grape-vine; ethylene; transgenic tobacco

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 1997
• Tom: 19
• Numer: 4
• Opis fizyczny: p.467-474,fig.

Twórcy

autor

Grimmig B

• National Research Center for Environment and Health, D-85764 Neuherberg, Germany

autor

Schubert R.

autor

Fischer R.

autor

Hain R.

autor

Schreier P.H.

autor

Betz C.

autor

Langebartels C.

autor

Ernst D.

autor

Sandermann H.

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