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1999 | 21 | 1 |

Tytuł artykułu

Graft and dodder transmission of phytoplasma affecting lily to experimental hosts

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Experimental infection of Alstroemeria seedlings with naturally infected lily ‘Casablanca’ with stunting and flower bud deficiency phytoplasma resulted 3–4 weeks after top grafting in chlorotic and/or necrotic stripes, whitening of the leaves, shoot necrosis and die back. Flower discoloration or malformation were not observed. Attempts to transmit phytoplasma from naturally infected lily and experimentally infected Alstroemeria to Catharanthus roseus by top grafting resulted in stunted growth, dull yellowing and malformation of the leaves in 4–6 weeks. Some plants were temporary entirely vegetative and did not produce flowers. The periwinkle plants that were bridged by Cuscuta odorata from the diseased lilies and Alstroemerias showed similar symptoms as top-grafted ones. With the universal primer pairs rU3/fU5 specific PCR product with expected length ∼900 was amplified from samples collected from lilies with severe symptoms and top grafted test plants. All PCR products used for RFLP analysis after digestion with Alu I showed the same restriction profiles. Position of three obtained bands corresponded to the lengths of the DNA fragments of American aster yellows (AAY) phytoplasma group.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

21

Numer

1

Opis fizyczny

p.21-26,fig.

Twórcy

autor
  • Research Institute of Pomology and Floriculture, Pomologiczna 18, 96-100 Skierniewice
autor

Bibliografia

  • Ahrens U., Seemüler E. 1992. Detection of DNA of plant pathogenic mycoplasmalike organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. Phytopathology 82: 828–832.
  • Bergs R., Cousin M.-T., Roux J., Mäurer R., Seemüller E. 1997. Detection of phytoplasma infection in declining Populus nigra ‘Italica’ trees and molecular differentiation of the aster yellows phytoplasmas identified in various Populus species. Eur. J. For. Path. 27: 33–43.
  • Bertaccini A., Marani F. 1982. Electron microscopy of two viruses and mycoplasma-like organisms in lilies with deformed flowers. Phytopath. Medit. 21: 8–14.
  • Bertaccini A., Vibio M., Bellardi M.G., Danielli A. 1996. Identification of phytoplasmas in Alstroemeria. Acta Hortic. 432: 312–319.
  • Dale J.L., Smith L.D. 1975. Mycoplasma like bodies observed in tomato plants with big bud in Arkansas. Plant Disease Reporter 59: 455–458.
  • Davies D.L., Guise C.M., Clark M.F., Adams A.N. 1992. Parry’s disease of pears is similar to pear decline and is associated with mycoplasma-like organisms transmitted by Cacopsylla pyricola. Plant. Pathology 41: 195–203.
  • Doi Y., Terenaka M., Yora K., Asuyama H. 1967. Mycoplasma or PLT group like micro-organisms found in the phloem elements of plants infected with mulberry dwarf, potato witches broom, aster yellows or paulownia witches broom. Ann. Phytopath. Soc. of Japan 33: 259–266.
  • Kamińska M., Malinowski T., Komorowska B., Rudzińska-Langwald A. 1996. Etiology of yellows and witches’ broom symptoms in some ornamental plants. Acta Hort. 432:96–106.
  • Kamińska M., Korbin M., Komorowska B., Puławska J. 1998. Stunting and flower buds deficiency of Lilium sp.: a new phytoplasma associated disease. Acta Physiol. Plant. 20: 49–53.
  • Kamińska M., Korbin M., Rudzińska-Langwald A. 1998. Occurrence and identification of aster yellows related phytoplasma in gladiolus in Poland. Phytopath. Polonica (in press).
  • Korbin M., Kamińska M. 1998. Characterization of cucumber mosaic cucumovirus isolates. Phytopath. Polonica (in press).
  • Lee I.-M. Klopmeyer M., Bartoszyk I.M., Gundersen-Rindal E., Chou T.-S., Thomson K.L., Eisenreich R. 1997. Phytoplasma induced free-branching in commercial poinsettia cultivars. Nature Biotechnology 15: 178–182.
  • Mäurer R., Seemüller E. 1996. Witches’ broom of Rhamnus cathartica: a new phytoplasma disease. J. Phytopathology 144: 221–223.
  • Marcone C., Ragozzino A., Seemüller E. 1997. Dodder transmission of alder yellows phytoplasma to the experimental host Catharanthus roseus (periwinkle). Eur. J. For. Path. 27: 347–350.
  • Schneider B., Ahrens U., Kirkpatrick B.C., Seemüller E. 1993. Classification of plant-pathogenic mycoplasma-like organisms using restriction-site analysis of PCR-amplified 16S rDNA. J. Gen. Microbiol. 139: 519–527.
  • Seemüller E.,Schneider B., Mäurer R., Ahrens U., Daire X., Kison H., Lorenz K.H., Firrao G., Avinent L., Sears B.B., Stackebrandt E. 1994. Phylogenetic classification of phytopathogenic mollicutes by sequence analysis of 16S ribosomal DNA. Int. J. Syst. Bacteriol. 44: 440–446.

Typ dokumentu

Bibliografia

Identyfikatory

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