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2009 | 58 | 4 |

Tytuł artykułu

Influence of culture conditions of Streptomyces sp. [strain S242] on chitinase production

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The purpose of this study was to determine the influence of growth conditions and medium composition on the production of chitinase by Strtptomyces sp. (strain S₂₄₂,). Production of chitinase by strain S₂₄₂ was detected on colloidal chitin agar (CCA) medium after 8 days of incubation at 28°C resulting in a clear zone 10 mm around the colony. Chitinase activity was assayed as the amount of N-acetylglucosamine released in pmol/ml/min using the dinitrosalicylic acid assay method. The crude enzyme had maximum activity (0.162 U ml/l) after 4 days of incubation at pH 7 and 30°C when the broth medium was supplemented with 1.6% of colloidal chitin. However, enzyme activity was strongly decreased at 40°C and extreme acidic and alkaline pH values. SDS-PAGE and zymogram analysis revealed six distinctive bands that range from 39 to 97 kDa with chitinolytic activity. The findings of this investigation create a possibility for the use of the organism in the commercial production of chitinase. In addition, it can be a source of DNA for cloning the chitinase gene(s) to generate phytopathogen resistant transgenic plants.

Wydawca

-

Rocznik

Tom

58

Numer

4

Opis fizyczny

p.339-345,fig.,ref.

Twórcy

autor
  • Jordan University of Science and Technology, P.O.Box 3030, Irbid -22110, Jordan
autor
autor
autor

Bibliografia

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  • Felse P.A. and T. Panda. 2000. Production of microbial chitinases - A revisit. Biopross. Eng. 23: 127-134
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  • Gupta R., R.K. Saxena, P. Chaturvedi and J.S. Virdi. 1995. Chitinase production by Streptomyces viridificans: it's potential in fungal cell wall lysis. J. Appl. Bacteriol. 78: 378-383.
  • Hsu S.K., and J.I. Lockwood. 1975. Powder chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl. Microbiol. 29: 422-426.
  • Mahadevan B. and D.L. Crawford D.L. 1997. Properties of chitinase of the antifungal biocontrol agent Streptomyces lydicus WYEC108. Em. Microb. Technol. 20: 489-493.
  • Miller G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chem. 31: 426-428.
  • Nampoothiri K.M., T.V. Baiju, C. Sandhya, A. Sabu, G. Szakacs and A. Pandey. 2004. Process optimization for antifungal chitinase production by Trichoderma harzianu. Process Biochem. 39: 1583-1590.
  • Nonomura H. 1974. Key for the classification of 458 species of streptomycetes included in the ISP. J. Ferm. Technol. 52: 78-92
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  • Saadoun L, L. Wahiby, Q. Ababneh, Z. Jaradat, M. Massadeh and F. AL-Momani. 2008. Recovery of soil streptomycetes from arid habitats in Jordan and their potential to inhibit multi-drug resistant Pseudomonas aeruginosa pathogens. World J. Microbiol. Biotechnol. 24: 157-162.
  • Schrempf H. 2001. Recognition and degradation of chitin by streptomycetes. Antonie van Leeuwenhoek 79: 285-289.
  • Shirling E.B. and D. Gottlieb. 1966. Methods for characterization of Streptomyces species. Inter. J. System. Bacteriol. 16: 313-340
  • Tahtamouni M.E.W., K.M. Hameed and I.M. Saadoun. 2006. Biological control of Sclerotinia sclerotiorum using indigenous chitinolytic actinomycetes in Jordan. Plant Pathol. J. 22: 107-114.
  • Tanabe T., T. Kawase, T. Watanabe, Y. Uchida and M. Mitsutomi. 2000. Purification and Characterisation of a 49-kDa Chitinase from Streptomyces griseus HUT 6037. J. Bios. Bioeng. 1: 27-32.
  • Trudel J. and A. Asselin. 1989. Detection of chitinase activity after Polyacrylamide gel electrophoresis. Analvt. Biochem. 178: 362-366.
  • Vinogradova S.P. and S.N. Kushnir. 2003. Biosynthesis of hydrolytic enzymes durig cocultivation of macro- and micromycetes. Appl. Biochem. Microbiol. 39: 573-575.
  • Watanabe T, W. Oyanagi, K. Suzuki and H. Tanaka. 1990. Chitinase system of Bacillus circulans WL-12 and importance of chitinase A1 in chitin degradation. J. Bacteriol. 172: 4017-4022
  • Williams S.T., M. Goodfellow, G. Alderson, E.M. Wellington, P.H. Sneath and M.J. Sackin M. 1983. Numerical classification of Streptomyces and related genera. J. Gen. Microbiol. 129: 1743-1813.
  • Williamson N., P. Brian and E.M.H. Wellington. 2000 Molecular detection of bacterial and streptomycetes chitinases in the environment. Antonie van Leeuwenhoek 78: 3 15-32 1.

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Bibliografia

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