Spectral properties of phthalocyanines incorporated into resting and stimulated human peripheral blood cells
Human peripheral blood cells stimulated by phytohemagglutinin (which serve as a model of cancerous cells) and resting cells were incubated in dimethyl sulfoxide solutions of various phthalocyanines. In order to diminish the influence of atmospheric oxygen the cells were embedded in a polymer (polyvinyl alcohol) film. Fluorescence spectra of the samples were measured over two regions of excitation wavelengths: at 405 nm (predominant absorption of the cell material) and in the regions of strong absorption of phthalocyanines (at about 605 nm and 337 nm). The intrinsic emission of cell material became changed as a result both of cells' stimulation and of incubation of cells in dye solution. In most cases the stimulated cells when stained by dye exhibited higher long wavelength fluorescence intensity than resting cells. This suggests higher efficiency of dye incorporation into cancerous cells than into healthy cells. The absorption spectra of samples were also measured. The spectra of various phthalo- cyanines in incubation solvent, in polymer and in the cells embedded in polymer, were compared. The comparison of properties of the cells stimulated for different time periods enabled to establish the conditions of stimulation creating a population of cells incorporating a large number of sensitizing molecules.
- Brancaleon L, Durkin AJ, Tu JH, Menaker G, Fallon JD, Kollias N. (2001) In vivo fluorescence spectroscopy of nonmelanoma skin cancer. Photochem Photobiol.; 73: 178-83 .
- Cubeddu R, Pifferi A, Taroni P, Torricelli A, Valentini G, Comelli D, D'Andrea C, Angelini V, Canti G. (2000) Fluorescence imaging during photodynamic therapy of experimental tumors in mice sensitized with disulfonated aluminium phthalocyanine. Photochem Photobiol.; 72: 690-5.
- Day PN, Wang Z, Pachter R. (1998) Calculation of the structure and absorption spectra of phthalocyanines in the gasphase and in solution. JMol Structure.; 455: 33-50.
- Dysart JS, Patterson MS, Farelli TJ, Singh G. (2002) Relationship between mTHPC fluorescence photobleaching and cell variability during in vitro photodynamic treatment of DP16 cells. Photochem Photobiol.; 75: 289-95.
- Frackowiak D, Niedbalska M, Wiktorowicz K. (1996) Influence of mitogenic activators on the structure of leukocytes. J Photochem Photobiol B: Biology.; 33: 45-50.
- Frackowiak D, Planner A, Ion RM, Wiktorowicz K. (1998) Incorporation of dye in resting and stimulated leukocytes. In Near-infrared dyes for high technology applications. Daehne S, Resch-Gennger U, Wolfbais OA. eds, pp 87-114. Kluver Academic Press.
- Frackowiak D, Planner A, Waszkowiak A, Boguta A, Ion RM, Wiktorowicz K. (2001a) Yield of phthalocyanines intersystem (singlet-triplet) crossing evaluated on the basis of time resolved photothermal method. J Photochem Photobiol A: Chemistry.; 141:101-8.
- Frackowiak D, Planner A, Wiktorowicz K. (2001b) Near infra-red applications in medicine. In Near-infrared applications in biotechnology Radhavachari R. ed, pp 151-83. M Dekker, New York, Brasel.
- Frackowiak D, Waszkowiak A, Manikowski H, Ion RM, Cofta J, Wiktorowicz K. (2001c) The interactions of phtalocyanines with stimulated and resting human peripheral blood mononuclear cells. Acta Biochim Polon.; 48: 257-69.
- Frackowiak D, Wiktorowicz K, Planner A, Waszkowiak A, Ion RM, (2001d) The phthalocyanines applications in photodynamic therapy investigated by time resolved and steady-state photothermal methods. Int JPhotoenergy.; 4: 51-6.
- Frackowiak D, Jon MR, Waszkowiak A. (2002) Spectral properties of phthalocyanines oriented in stretched polymer films. JPhys Chem.; (in press).
- Ion RM, Planner A, Wiktorowicz K, Frackowiak D. (1998) The incorporation of various porphyrins into blood cells measured via flow cytometry absorption and emission spectroscopy. Acta Biochim Polon.; 45: 833-45.
- Ion RM. (1999) Implications of supermolecular aggregates of MeP and MePc in PDT . Proceedings of International Conference of Bioorganic Chemistry, Greece 123p.
- Lakowicz JR. (1999) Principles of Fluorescence Spectroscopy. 2 edn, pp 63-7, Kluwer Academic/Plenum Publishers, New York, Boston Dordrecht, London, Moscow.
- Ostler RB, Scully AD, Taylor AG, Gould IR, Smith TA, Waite A, Phillips D. (2000) The effect of pH on the photophysics and photochemistry of disulphonated aluminum phthalocyanine. Photochem Photobiol.; 71: 397-404.
- Pogue BW, Pitts JD, Mycek MA, Sloboda RD, Wilmot CM, Brandsema JF, O'Hara JA. (2001) In Vivo NADH fluorescence monitoring as an assay for cellular damage in photodynamic therapy. Photochem Photobiol.; 74: 817-24.
- Rosenthal I. (1991) Phthalocyanines as photodynamic sensitizers. Photochem Photobiol.; 53:859-70.
- Trivedi N, Wang H-W, Nieminen AL, Oleinick NL, Izatt JA. (2000) Quantitative analysis of PC 4 localization in mouse lyphoma (LY-R) cells via double-label confocal fluorescence microscopy. Photochem Photobiol.; 71: 634-9.
- Wood SR, Holroyd JA, Brown SB. (1997) The subcellular localization of Zn(II) phthalocyanines and their redistribution on exposure to light. Photochem Photobiol.; 65: 397-402.
- Yang Y, Katz A, Celmer I, Żurawska- Szczepaniak M, Alfano R. (1997) Fundamental differences of excitation spectrum between malignant and benign breast tissues. Photochem Photobiol.; 66: 518-22.