Regeneration of leaf mesophyll protoplasts from Lycopersicon glandulosum, L. peruvianum and L. esculentum Stevens x Rodade hybrid

• Autorzy: Pindel A. , Lech M. , Miczynski K.
• Języki publikacji: EN

Abstrakty

EN

Protoplasts were isolated from mesophyll leaf tissue of in-vitro-grown 3-4-week-old seedlings of two wild tomato species L. glandulosum C.H. Mull. PI 731163 and L. peruvianum (L.) Mill. PI 128650, and from F4 plants of the Stevens x Rodade hybrid of L. esculentum Mill. For protoplasts isolation from both wild species a mixture of 0.3% cellulase + 0.03% macerozyme + 0.01% MES and 7.0% sorbitol was used, whereas maceration of leaf pieces from Stevens x Rodade was performed in a mixture of 0.3—1.0% cellulase + 0.2% macerozyme + 0.01% MES + 0.05% glycine and 7.0% sorbitol. Protoplast culture was carried out in a slightly modified nutrient medium of Tan et. al. (1987b). The yield of protoplasts per 1 g tissue amounted to 9 x 107 for both wild species, and 4 x 108 for the Stevens x Rodade plants. The first cell divisions of protoplasts from all three studied species occurred after 6-10 days of culture. Regenerated shoots rooted in 90% of both wild Lycopersicon species. Regeneration of Stevens x Rodade did not pass the greening callus stage.

Słowa kluczowe

EN

cucumber mosaic virus; Lycopersicon esculentum; Stevens x Rodade hybrid; somatic hybridization; callus; hybridization; protoplast; Lycopersicon glandulosum; regeneration; wild tomato; Lycopersicon peruvianum; plant

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 1998
• Tom: 40
• Opis fizyczny: p.41-46,fig.

Twórcy

autor

Pindel A.

• Agricultural University, al.29 Listopada 54, 31-425 Krakow, Poland

autor

Lech M.

autor

Miczynski K.

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