The cytoplasmic domain of chondrolectin interacts with the beta-subunit of Rab geranylgeranyl transferase

• Autorzy: Claessens A , Weyn C. , Merregaert J.
• Języki publikacji: EN

Abstrakty

EN

Mouse chondrolectin (chodl) was isolated out of the tail tip of fourday old 129/SvJ mice as a by-product of a PCR-based subtractive cDNA library screening. The gene is predominantly expressed in adult skeletal muscle, heart, testes and lungs and in embryonic stadia. Chodl is the mouse homologue of human chondrolectin (CHODL), a gene that encodes for a type Ia transmembrane protein and that is expressed in human testis, prostate, heart and skeletal muscle tissue. CHODL-splice variants (CHODLf, CHODLfΔE, CHODLΔE) are detected in human leukocytes. The proteins of the chondrolectin family belong to the family of C-type lectins. As the members of this protein family are important for a wide array of biological processes, the function of chodl was investigated by searching for its protein interaction partners. The β-subunit of Rab geranylgeranyl transferase (Rabggtb) was isolated 8 times after a complete Sos recruitment system (SRS) screen with the cytoplasmic domain of chodl. The interaction was confirmed with in vitro transcription/translation and co-immunoprecipitation (co-IP) experiments

Słowa kluczowe

EN

C-type lectin; chondrolectin; man; mouse; mice; Sos recruitment system; cDNA library; skeletal muscle; heart; lung; transmembrane protein; leucocyte; protein family; biological process; beta-subunit; geranylgeranyl transferase

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2008
• Tom: 13
• Numer: 2
• Opis fizyczny: p.250-259,fig.,ref.

Twórcy

autor

Claessens A

• University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium

autor

Weyn C.

autor

Merregaert J.

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