gyrA mutations in ciprofloxacin-resistant clinical isolates of Pseudomonas aeruginosa in a Silesian hospital in Poland

• Autorzy: Wydmuch Z , Skowronek-Ciolek O. , Cholewa K. , Mazurek U. , Pacha J. , Kepa M. , Idzik D. , Wojtyczka R.D.
• Języki publikacji: EN

Abstrakty

EN

Among 73 clinical isolates of Pseudomonas aeruginosa 48 strains were Ciprofloxacine (CIP) susceptible and 25 CIP resistant (Minimal inhibitory concentration - MIC>32 μg/ml - 14 strains) or of intermediate susceptibility to CIP (MIC≥ 1,5-32 μg/ml - 11 isolates). Mutations in the quinolone-resistance-determining region (QRDR) of gyrA gene were searched in groups of CIP resistant and of intermediate susceptibility to CIP isolates. Two methods: restriction fragment length polymorphism (RFLP) analysis and DNA sequencing analysis allowed to detect three different mutations. The nucleotide substitutions observed led to the following amino acid replacements: Thr-83 —> Ile, Asp-87—>Asn, Asp-87—>Gly. One mutated strain among the group of mutants analyzed showed double mutation (Thr-83—>Ile, Asp-87—>Gly) and additional silent mutation (Val-103—> Val); whilst the rest of the isolates showed different single missense mutations. The most frequently detected mutation in the gyrA gene (16 out of 25 mutants) was the Thr-83—>Ile substitution.

Słowa kluczowe

EN

ciprofloxacin; gyrA mutation; clinical isolate; Poland; Pseudomonas aeruginosa; mutation; Silesian hospital; microbiology; resistance

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2005
• Tom: 54
• Numer: 3
• Opis fizyczny: p.201-206,fig.,ref.

Twórcy

autor

Wydmuch Z

• Medical University of Silesia, Sosnowiec, Poland

autor

Skowronek-Ciolek O.

autor

Cholewa K.

autor

Mazurek U.

autor

Pacha J.

autor

Kepa M.

autor

Idzik D.

autor

Wojtyczka R.D.

Bibliografia

• Akasaka T., M. Tanaka, A. Yamaguchi and K. Sato. 2001. Type II topoisomerase mutations in fluoroquinolone-resistant clinical strains Pseudomonas aeruginosa isolated in 1998 and 1999: role of target enzyme in mechanism of fluoroquinolone resistance. Antimicrob. Agents Chemother. 45: 2263-2268.
• Ball P. 1994. Bacterial resistance to fluoroquinolones: lessons to be learned. Infection 22: 140-147.
• Bearden D.T. and L.H. Danziger. 2001. Mechanism of action and of resistance to quinolones. Pharmacotherapy. 21: 224S-232S.
• Drilica K. and X. Zhao. 1997. DNA gyrase, topoisomerase IV, and the 4-quinolones. Microbiol. Mol. Biol. Rev. 61: 377-392.
• Kureishi A., J.M. Diver, B. Beckthold, T. Schollaardt, L.E. Bryan. 1994. Cloning and nucleotide sequence of Pseudomonas aeruginosa DNA gyrase gyrA gene from strain PAOl and quinolone-resistant clinical isolates. Antimicrob. Agents Chemother. 38: 1944-1952.
• Mouneimné H., J. Robert, V. Jarlier and E. Cambau. 1999. Type II topoisomerase mutations in ciprofloxacin-resistant strains of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 43: 62-66.
• Nakajima A., Y. Sugimotoo, H. Yoneyama and T. Nakae. 2002. High-level fluoroquinolone resistance in Pseudomonas aeruginosa due to interplay of the MexAB-OprM efflux pump and the DNA gyrase mutation. Microbiol. Immun. 46: 391-395.
• Nakano M., T. Deguchi, T. Kawamura, M. Yasuda, M. Kimura, Y. Okano and Y. Kawada. 1997. Mutations in the gyrA and parC genes in fluoroquinolone-resistant clinical isolates of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 41: 2289-2291.
• National Committee for Clinical Laboratory Standards. 2000. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 5th edn. Approved standard. NCCLS document M7-A5. Wayne, Pennsylvania.
• Takenouchi T., E. Sakagawa, M. Sugawara. 1999. Detection of gyrA mutations among 335 Pseudomonas aeruginosa strains isolated in Japan and their susceptibilities to fluoroquinolones. Antimicrob. Agents Chemother. 43: 406-409.
• Weigel L.M., Ch.D. Steward and F.C. Tenover. 1998. gyrA mutations associated with fluoroquinolone resistance in eight species of Enterobacteriaceae. Antimicrob. Agents Chemother. 42: 2661-2667.
• Yonezawa M., M. Takahata, N. Matsubara, Y. Watanabe and H. Narita. 1995. DNA gyrase gyrA mutations in quinolone-resistant clinical isolates of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 39: 1970-1972.
• Yoshida H., M. Bogaki, M. Nakamura and S. Nakamura. 1990. Quinolone resistance-determining region in the DNA gyrase gyrA gene of Escherichia coli. Antimicrob. Agents Chemother. 34: 1271-1272.