Typowanie szczepow Pseudomonas aeruginosa opornych na karbapenemy technika PCR-RAPD

• Autorzy: Bogiel T. , Gospodarek E.

Warianty tytułu

EN

PCR-RAPD typing of carbapenem-resistant Pseudomonas aeruginosa strains

• Języki publikacji: PL

Abstrakty

PL

W związku ze znacznym wzrostem w 2007 roku liczby izolacji szczepów Pseudomonas aeruginosa opornych na karbapenemy, przeprowadzono ge- notypowanie techniką PCR-RAPD 16 wybranych izolatów z materiału od chorych leczonych w Szpitalu Uniwersyteckim nr 1 im. dr. A. Jurasza w Bydgoszczy. W roku objętym badaniem od 78 chorych izolowano 125 szczepów P. aeruginosa o fenotypie oporności na imipenem i meropenem. Wykazano, że wszystkie badane szczepy były odmienne, dowodząc przydatności techniki PCR-RAPD w typowaniu szczepów pałeczek ropy błękitnej.

EN

P. aeruginosa rods are opportunistic pathogens responsible generally for nosocomial infections. Resistance to carbapenems, observed among them, is a serious threat due to ability to be transmitted between bacterial species. The aim of our study was to evaluate the usefulness of PCR-RAPD technique in typing of 16 carbapenem-resistant P. aeruginosa strains isolated in 2007 from different patients of University Hospital No. 1 of dr A. Jurasz Collegium Medicum of L. Rydygier in Bydgoszcz Nicolaus Copernicus University in Toruń. Study shows increasing frequency of isolation that type of strains when compared to 2006. Percentage of carbapenem-resistant isolates raised from 12,4% in 2006 to 22,9% in 2007. The majority of examined strains were obtained from patients of the Intensive Care Units (25,0%) and were isolated from bronchoalveolar lavage (25,0%), urine (25,0%) and wound swabs ( 18,8%) samples. Examined P. aeruginosa strains demonstrated resistance to doripenem (81,3%) and piperacillin (75,0%) and susceptibility to Colistin ( 100,0%), amikacin (81,3%), netilmicin and norfloxacin (75,0% each). Using PCR-RAPD amplification with 208 and 272 primers, 14 and 16 DNA patterns were obtained, respectively. Usefulness of PCR-RAPD in carbapenem-resistant P. aeruginosa strains typing was proved in case of strains presenting similar and/or different antimicrobials susceptibility patterns.

Słowa kluczowe

PL

zakazenia szpitalne; Pseudomonas aeruginosa; opornosc na antybiotyki; antybiotyki karbapenemowe; szczepy bakteryjne; lancuchowa reakcja polimerazy; metoda RAPD; izolaty bakteryjne

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2010
• Tom: 62
• Numer: 3
• Opis fizyczny: s.211-219,rys.,fot.,bibliogr.

Twórcy

autor

Bogiel T.

• Collegium Medicum w Bydgoszczy, Uniwersytet Mikolaja Kopernika w Toruniu, ul.M.Sklodowskiej-Curie 9, 85-094 Bydgoszcz

autor

Gospodarek E.

Bibliografia

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