Effect of Botrytis cinerea infection and elicitation on beta-1,3-glucanase and chitinase activity in bean leaves and cell cultures

• Autorzy: Kuzniak E , Urbanek H. , Michalak A. , Herka K.

Warianty tytułu

PL

Wplyw zakazenia Botrytis cinerea i elicytacji na aktywnosc beta-1,3-glukanazy i chitynazy w lisciach i kulturach zawiesinowych fasoli

• Języki publikacji: EN

Abstrakty

EN

The activity of ß-1,3-glucanase and chitinase in bean plants treated with B. cinerea products or/and infected and in cell cultures after application of fungal products has been studied. Botrytis cinerea infection and culture filtrates, ethanol precipitates, glucan and conidial extract treatment markedly enhanced the activity of both hydrolases. Cell cultures treated with B. cinerea products reacted similarly to intact plants. In plants pretreated with 2-day culture filtrate and conidial extract and then infected, ß-1,3-glucanase and chitinase were induced stronger than after infection without pretreatment.

PL

W pracy badano aktywność 3-1,3-glukanazy i chitynazy w liściach fasoli (Phaseolus vulgaris L.) odm. „Złota Saxa" hodowanej w szklarni po potraktowaniu produktami otrzymanymi z hodowli Botrytis cinerea Pers. lub/i po zakażeniu oraz w kulturach zawiesinowych traktowanych produktami grzyba. Stwierdzono wyraźny wzrost aktywności obu enzymów w liściach zarówno po zakażeniu, jak i po potraktowaniu ekstraktem z zarodników, filtratami, osadami alkoholowymi i glukanem z hodowli B. cinerea Pers. Reakcja kultur zawiesinowych fasoli pod wpływem produktów B. cirenea Pers. była podobna do stwierdzonej w liściach. Indukcja p-l,3-glukanazy i chitynazy w liściach uprzednio traktowanych ekstraktem z zarodników i filtratem z 2-dniowej hodowli grzyba, a następnie zakażonych była silniejsza niż w liściach nietraktowanych po zakażeniu.

Słowa kluczowe

EN

Zlota Saxa cultivar; bean; enzyme activity; beta-1,3-glucanase; leaf; Botrytis cinerea; fungal infection; chitinase; plant cultivar

PL

fasola Zlota Saxa; fasola; aktywnosc enzymatyczna; beta-1,3-glukanazy; liscie; Botrytis cinerea; infekcja grzybowa; chitynaza; odmiany roslin

• Wydawca: -
• Czasopismo: Acta Agrobotanica
• Rocznik: 1994
• Tom: 47
• Numer: 2
• Opis fizyczny: s.63-71,rys.,tab.,bibliogr.

Twórcy

autor

Kuzniak E

• Uniwersytet Lodzki, Banacha 12/16, 90-237 Lodz

autor

Urbanek H.

• Uniwersytet Lodzki, Banacha 12/16, 90-237 Lodz

autor

Michalak A.

• Uniwersytet Lodzki, Banacha 12/16, 90-237 Lodz

autor

Herka K.

• Uniwersytet Lodzki, Banacha 12/16, 90-237 Lodz

Bibliografia

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