DNA replication reaction in Xenopus cell-free system is suppressed by high pressure

• Autorzy: Takahashi H , Yamaguchi T. , Koga M. , Kageura H. , Terada S.
• Języki publikacji: EN

Abstrakty

EN

Previously, we demonstrated that when mouse erythroleukemia cells are exposed to a pressure of 80 MPa, the cell-cycle progression of S-phase cells is retarded. To examine the effects of high pressure on DNA replication, we used a Xenopus cell-free system. From cell-cycle progression of sperm nuclei, it was found that sperm nuclei are stable to a pressure of 80 MPa, whereas egg extracts are susceptible to high pressure. Similarly, biotin-16-dUTP was incorporated into 80 MPa-treated sperm nuclei in pressure-untreated extracts, but not into naive sperm nuclei in 80 MPa-treated extracts. These results indicate that DNA replication in Xenopus cell-free system is suppressed by the susceptibility of the extracts to a pressure of 80 MPa.

Słowa kluczowe

EN

cell cycle; high pressure; DNA replication; Xenopus; cell-free system

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2004
• Tom: 09
• Numer: 3
• Opis fizyczny: p.423-427,fig.,ref.

Twórcy

autor

Takahashi H

• Fukuoka University, Jonan-ku, Fukuoka, 814-80 Japan

autor

Yamaguchi T.

autor

Koga M.

autor

Kageura H.

autor

Terada S.

Bibliografia

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