The effects of indomethacin on angiogenic factors mRNA expression in renal cortex of healthy rats

• Autorzy: Mucha K. , Foroncewicz B. , Koziak K. , Czarkowska-Paczek B. , Paczek L.
• Języki publikacji: EN

Abstrakty

EN

Indomethacin is a nonsteroidal anti-inflammatory drug used frequently to control chronic or temporary pain. In the kidney, indomethacin decreases medullary and cortical perfusion, resulting in hypoxia. Kidney hypoxia has many effects, including changes in gene expression, and is a strong stimulus for angiogenesis. Other angiogenic factors include vascular endothelial growth factor (VEGF), basic fibroblast growth factor (FGF-2), transforming growth factor beta 1 (TGFß1), and platelet-derived growth factor (PDGF). Our goal was to examine the influence of indomethacin on mRNA expression of these factors and their selected receptors in the renal cortex of healthy rats. Groups of 8 healthy, male, six-week-old Wistar rats received either indomethacin (5 mg/kg/day) or placebo orally for three months. RNA from renal cortex biopsies was analyzed by real-time polymerase chain reaction to quantify the mRNA levels of each cytokine. We observed significantly higher mRNA levels for VEGF (1.73-fold), FGF-2 (5.6-fold) and TGFß receptor III (2.93-fold), PDGF receptor alpha (2.93-fold) and receptor ß (2.91-fold) in rats receiving indomethacin compared to rats given placebo (p < 0.05). Amounts of mRNA for TGFß1, PDGF, FGF receptors 1 and 2 and TGFß receptor I did not differ between analysed groups. Our data indicates that indomethacin may regulate the expression of potent angiogenic factors VEGF and FGF-2.

Słowa kluczowe

EN

fibroblast growth factor-2; angiogenic factor; platelet-derived growth factor; indomethacin; kidney; renal cortex; mRNA expression; transforming growth factor-beta-1; rat; vascular endothelial growth factor

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2007
• Tom: 58
• Numer: 1
• Opis fizyczny: p.165-178,fig.,ref.

Twórcy

autor

Mucha K.

• Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, Poland

autor

Foroncewicz B.

autor

Koziak K.

autor

Czarkowska-Paczek B.

autor

Paczek L.