Effect of laser biostimulation on cell proliferation in the healing of cutaneous surgical wounds in pigs

• Autorzy: Chyczewski M , Rozicka A. , Mikolajczyk A. , Rotkiewicz T. , Holak P. , Jalynski M. , Rotkiewicz Z.
• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to investigate the effect of low-level laser therapy (LLLT) on the proliferation of epithelial and connective tissue cells in the healing of incised cutaneous wounds in pigs. The experiment was conducted on 12 young pigs divided into four groups. Group I (undamaged skin) and group III (damaged skin) served as control. Group II - pigs with undamaged skin and group IV - pigs with incised wounds in the dorsal area were subjected to laser irradiation. Laser biostimulation was carried out using a CTL 1106 MX semiconductor laser in the continuous wave mode of operation at a wavelength of 810 nm and a maximum output of 100 mW. Following three weeks of observation, skin specimens were collected for histopathological analysis (HE), immunohistochemical detection of PCNA, and determination of apoptosis (TUNEL) and presence of mast cells (toluidine blue staining). Laser irradiation administered at E=8 J/cm for 1 min over a period of three weeks accelerated the proliferation of stratum basale cells, stimulated fibroblast proliferation, increased the number of mast cells in the wound area, and inhibited apoptosis in cells participating in the skin regeneration process.

Słowa kluczowe

PL

pig; surgical wound; laser biostimulation; laser therapy; cell proliferation; apoptosis; skin regeneration; tissue regeneration

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 2
• Opis fizyczny: p.217-221,fig.,ref.

Twórcy

autor

Chyczewski M

• University of Warmnia and Mazury in Olsztyn, 10-957 Olsztyn, Poland

autor

Rozicka A.

autor

Mikolajczyk A.

autor

Rotkiewicz T.

autor

Holak P.

autor

Jalynski M.

autor

Rotkiewicz Z.

Bibliografia

• 1. Chyczewski M., Brzeski W., Jałyński M;, Woźniak P., Rotkiewicz T.: The influence of laser biostimulation on bone fracture healing. Magazyn Wet 1998, 2, 99-101.
• 2. Chyczewski M., Jałyński M., Nowicki M., Brzeski W.: Laser biostimulation in neurological diseases of dogs. Ann Univ Mariae Curiae-Sklodowska (Vet) 2005, 60, 55- 58.
• 3. Chyczewski M., Holak P., Jałyński M., Kasprowicz A., Rotkiewicz T., Mikołajczyk A.: Effect of laser biostimulation on the healing of cutaneous surgical wounds in pigs. Bull Vet Inst Pulawy 2009, 53, 135-138.
• 4. Fiedor P.: Zarys klinicznych zastosowań laserów. Ed. Dom Wydawniczy Ankar, Warszawa, 1995.
• 5. Galletti G., Bolognani L., Ussia G.: Laser applications in medicine. Proc 3rd World Congress - International Society for Laser Applications in Medicine, Editor Monduzzi, Bologna, 1992.
• 6. Haas A.E., Isseroff R.R., Wheeland R.G., Rood P.A., Graves P.J.: Low-energy helium-neon laser irradiation increases the motility of cultured human kerationcytes. J Invest Dermatol 1990, 94, 822-826.
• 7. Kana J.S., Hutschenreiner G., Haina D., Waidelich W.: Effect of low-power density laser radiation on healing of open skin wonds in rats. Arch Surg 1981, 116, 293-296.
• 8. Karu T.: Low-intensity laser light action upon fibroblasts and lymphocytes. Oshiro T., Calderhead R.G.: Progress in laser therapy. Wiley and Sons, New York, 1991.
• 9. Pessoa E.S., Melhado R.M., Theodoro L.H., Garcia V.G.: A histologic assessment of the influence of low-intensity laser therapy on wound healing in steroid-treated animals. Photomed Laser Surg 2004, 22, 199-204.
• 10. Rocha Junior A.M., Vieira B.J., de Andrade L.C., Aarestrup F. M.: Low-level laser therapy increases transforming growth factor-ß-2 expression and induces apoptosis of epithelial cells during the tissue repair process. Photomed Laser Surg 2009, 27, 303-307.
• 11. Viegas V.N., Abreu M.E., Viezzer C., Machado D.C., Filho M.S., Silva D.N., Pagnoncelli R.M.: Effect of low- level laser therapy on inflammatory reactions during wound healing: comparison with meloxicam. Photomed Laser Surg 2007, 25, 467-473.