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2005 | 46 | 3 |

Tytuł artykułu

The influence of several factors on the efficiency of androgenesis in carrot

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The influence of cultivar, donor plant and culture procedure on the efficiency of androgenesis was studied in carrot anther culture. Experiments were carried out on five carrot cultivars: CxC 9900 F₁, Lucky B F₁, HCM, Beta III and Perfekcja, which were chosen because of their high carotene contents. Two procedures of anther culture were compared: (1) incubation in darkness for two weeks, followed by exposure to continuous light and transfer onto a fresh medium of the same composition; and (2) incubation in darkness until embryos appeared, without transfer onto a fresh medium. Temperature was +27°C all the time. Genotype played an important role in the process of androgenesis in carrot anther culture.The efficiency was the highest in cv. HCM - 5.6 embryos per 100 anthers. Considerable differences in the capacity for androgenesis were observed between individual donor plants. The ratio of embryos obtained per 100 anthers for cv. HCM varied from 0.0 to 48.9. The second procedure of anther culture proved to be more efficient, cheaper and less complicated.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

46

Numer

3

Opis fizyczny

p.265-269,fig.,ref.

Twórcy

autor
  • Research Institute of Vegetable Crops, 96-100 Skierniewice, Poland
autor

Bibliografia

  • Andersen SB, Christiansen J, Farestveit B, 1990. Carrot (Daucus carota L.): In vitro production of haploids and field trials. In: Bajaj YPS, ed. Biotechnology in Agriculture and Forestry, Springer Verlag, Berlin 12: 393-402.
  • Arnison PG, Donaldson P, Jackson A, Semple Ch, Keller WA, 1990. Genotype - specific response of cultured broccoli (Brassica oleracea var. italica) anthers to cytokinins. Plant Cell Tiss and Org Cult 20: 217-222.
  • Bajaj YPS, 1990. In vitro production of haploids and their use in cell genetics and plant breeding. In: Bajaj YPS, ed. Biotechnology in Agriculture and Forestry, Springer Verlag, Berlin 12: 3-44.
  • Gamborg OL, Miller RA, Ojima O, 1968. Nutrient requirements of suspension cultures of soybean root cell. Exp Cell Res 50: 151-158.
  • Górecka K, Krzyżanowska D, Śmiech M, Hoser-Krauze J, 1996. The effect of genotype on androgenesis and regeneration of plants from anther derived embryos in Brussels sprouts. J Appl Genet 37A: 191-194.
  • Górecka K, 1998. Obtaining of homozygous lines of head cabbage (Brassica oleraceae L. var. capitata L.) with aid of anther culture. (Polish with English summary) Habil Thesis, 14 ed. Res Inst Veg Crops): 1-71.
  • Hu KL, Matsubara S, Murakami K, 1993. Haploid plant production by anther culture in carrot (Daucus carota L.) J Jap Soc Hort Sci 62: 561-565.
  • Keller WA, Rajhathy R, Lacapra J, 1975. In vitro production of plants from pollen in Brassica campestris. Can J Genet Cytol 17: 655-666.
  • Keller WA, Armstrong KC, 1977. Embryogenesis and plant regeneration in Brassica napus anther cultures. Can J Bot 55: 1383-1388.
  • Keller WA, Armstrong KC, 1979. Stimulation of embryogenesis and haploid production in Brassica campestris anther cultures by elevated temperature treatments. Theor Appl Genet 55: 65-67.
  • Matsubara S, Dohya N, Murakami K, Nishio T, Dore C, 1995. Callus formation and regeneration of adventitious embryos from carrot, fennel and mitsuba microspores by anther and isolated microspore cultures. Acta Hort 392: 129-137.
  • Nałęczyńska A, 1991. Application of doubled haploids in breeding of rape. Part I. Obtaining of homozygous lines. Hod Rośl Aklim (Polish with English summary) 35: 3-28.
  • Ockendon DJ, 1986. Utilization of anther culture in breeding Brussels sprouts. In: Horn W, Hensen CJ, Odenbach W, Scheider O, eds. Genetic manipulation in plant breeding. Walter de Gruyter Berlin: 265-271.
  • Osolnik B, Bohanec B, Jelaska S, 1993. Stimulation of androgenesis in white cabbage Brassica oleraceo var. capitata anthers by low temperature and anther dissection. Plant Cell Tiss Org Cult 32: 241-246.
  • Tyukavin GB, Shmykova NA, Mankhova MA, 1999. Cytological study of embryogenesis in cultured carrot anthers. Russ J Plant Phys 46: 876-884.
  • Siebel J, Paulus KP, 1989. A comparison of anther and microspore culture as a breeding tool in Brassica napus. Theor Appl Genet 78: 473-479.
  • Yang O, Chauvin JE, Herve Y, 1992. A study of factors affecting anther culture of cauliflower Brassica oleracea var. botrytis. Plant Cell Tiss Org Cult 28: 289-296.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-ed04ce9d-4a2f-4d14-9b52-ced16aced39e
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