Kinetic studies on the oxidation of nitrite by horseradish peroxidase and lactoperoxidase

• Autorzy: Gebicka L.
• Języki publikacji: EN

Abstrakty

EN

The reaction of nitrite (NO-2) with horseradish peroxidase and lactoperoxidase was studied. Sequential mixing sopped-flow measeruments gave the following values for the rate constants of the reaction of nitrite with compounds II (oxoferryl heme intermediates) of horseradish peroxidase and lactoperoxidase at pH 7.0, 13.3 ± 0.07 mol-1dm3s-1 and 3.5 ± 0.05 · 104mol-1dm3s-1, respectively. Nitrite, at neutral pH, influenced measurements of activity of lactoperoxidase with typical substrates like 2,2'-azino-bis[ethyl-benzothiazoline-(6)-sulphonic acid] (ABTS), guaiacol or thiocyanate (SCN-). The rate of ABTS and guaiacol oxidation increased linearly with nitrite concentration up to 2.5-5 mmol dm-3. On the other hand, two-electron SCN- oxidation was inhibited in the presence od nitrite. Thus, nitrite competed with the investigated substrates of lactoperoxidase. The intermediate, most probably nitrogen dioxide (*NO2), reacted more rapidly with ABTS or guaiacol than did lactoperoxidase compound II. It did not, however, effectively oxidize SCN- to OSCN-. NO-2 did not influence the activity measurements of horseradish peroxidase by ABTS or guaiacol method.

Słowa kluczowe

EN

horseradish; lactoperoxidase; oxidation; stopped-flow measurement; nitrite; peroxidase

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 4
• Opis fizyczny: p.919-927,fig.

Twórcy

autor

Gebicka L.

• Technical University of Lodz, W.Wroblewskiego 15, 93-590 Lodz, Poland

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