An attempt to protect winter wheat against Gaeumannomyces graminis var.tritici by the use of rhizobacteria Pseudomonas fluorescens and Bacillus mycoides

• Autorzy: Czaban J , Ksiezniak A. , Wroblewska B. , Paszkowski W.L.
• Języki publikacji: EN

Abstrakty

EN

Pseudomonas fluorescens strains III107 and II21 and Bacillus mycoides strains JC192 and K184, stimulating growth of winter wheat, were chosen for the studies. The bacterial strains inhibited on agar nutrient medium the growth of Gaeumannomyces graminis var. tritici (Ggt) - the pathogenic fungus causing take-all on wheat. Both strains of pseudomonads synthesized relatively high amounts of Fe³⁺ chelators. The strains of bacilli were characterized by the very fast spreading on agar media. Furthermore, strain II21 was highly cyanogenic, and strain JC192 highly chitinolytic. Bacterization of winter wheat seeds (especially with strains III107 and JC192) significantly reduced the percentage of the plants infested with the pathogen in the 28 day glasshouse pot experiment. In the plot experiment, the winter wheat seeds were inoculated with a mixture of strains III107, II21 and JC192. Due to the bacterization the yield of wheat grain and straw was higher in comparison to the series with Ggt alone by 122% and 75%, respectively, but it amounted only to 45% and 43% of the control series not contaminated with Ggt. The decrease of percentage of wheat ears with weight less than 500 mg from 61% in Ggt-series to 25% in Ggt-bacterized-series, and especially the decrease of percentage of wheat ears with weight less than 200 mg from 43% to 14% additionally indicate the partial protection of the winter wheat against Ggt by the rhizobacteria. In the experimental series not contaminated with Ggt the percentage of these wheat ears fractions did not exceed 3% and 0.5%, respectively.

Słowa kluczowe

EN

plant disease; Pseudomonas fluorescens; Bacillus mycoides; wheat; rhizobacteria; Triticum aestivum; root disease; winter wheat; Gaeumannomyces graminis var.tritici

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2004
• Tom: 53
• Numer: 2
• Opis fizyczny: p.101-110,fig.,ref.

Twórcy

autor

Czaban J

• Institute of Soil Science and Plant Cultivation, 8 Czartoryskich St., 24-100 Pulawy, Poland

autor

Ksiezniak A.

autor

Wroblewska B.

autor

Paszkowski W.L.

Bibliografia

• Bednářová-Civínová M., V. Petříková, M. Stanek and V. Vančura. 1981. The influence of seed bacterization on Gaeumannomyces graminis, growth and yield of wheat, (in Czech) Sbor. ÚVTIZ-Ochr. Rostl. 17: 88-96.
• Czaban J. 2001. Saprophytic microflora of winter wheat rhizosphere (in Polish). PhD thesis, IUNG Puławy.
• de Boer M., I. van der Sluis, L.C. van Loon and P.A.H.M. Bakker. 1997. In vitro compatibility between fluorescent Pseudomonas spp. strains can increase effectivity of Fusarium wilt control by combinations of these strains, p. 380-382. In: A. Ogoshi, K. Kabayashi, Y. Homma, F. Kodama, N. Kondo, S. Akino (eds), Plant Growth-Promoting Rhizobacteria. Present Status and-Future Prospects. Sapporo, Japan.
• Duffy B.K., A. Simon and D.M. Weller. 1996. Combination of Trichoderma koningii with fluorescent pseudomonads for control of take-all on wheat. Phytopathology 86: 188-194.
• Hornby D. 1998. Take-all disease of cereals. CABI Oxon, New York.
• Jaroszuk-Ściseł J., E. Kurek. 2001. Biocontrol of rye fusariosis by Pseudomonas fluorescens under field conditions. Bull. Pol. Acad. Sci. Biol. Sci. 49: 275-285.
• Keel C, D. Haas, J. Lavilie, M. Maurhofer, TH. Oberhänsli, U. Schnider, C. Voisard and G. Defago. 1990. p. 195-196. In: C. Keel, B. Koller and G. Défago (eds), Plant Growth-Promoting Rhizobacteria Progress and prospects, Interlaken, Switzerland.
• Kim D.-S., R.J. Cook and D.M. Weller. 1997. Bacillus sp. L324-92 for biological control of three root diseases of wheat grown with reduced tillage. Phytopathology 87: 551-558.
• Kobus J., J. Czaban, A. Gajda, D. Masiak and A. Księżniak. 1993. Wheat rhizosphere microflora and its effect on plant nutrition and some pathogenic fungi, part I. Changes of rhizobacterial populations with development of winter wheat. Roczn. Glebozn. 44: 45:53.
• Księżniak A., J. Kobus. 1993. The participation of the rhizosphere microflora of wheat, barley and oat in the siderophore production (in Polish). Pam. Pul. 102: 77-90.
• Kuś J. and A. Mróz. 1996. Root pathogen infestation and yielding of some winter wheat variety in relation on forecrop (in Polish), p. 265-270. In: Materiały z Sympozjum „Nowe kierunki w fitopatologii" Kraków, 11-13 września 1996.
• Lemanceau P., P.A.H.M. Bakker, W.J. Dekogel, C. Alabouvette and B. Schippers. 1992. Effect of Pseudobactin 358 production by Pseudomonas putida WCS358 on suppression of Fusarium wilt of carnations by non-pathogenic Fusarium oxysporum Fo47. Appl. Environ. Microbiol. 59: 74-82.
• Mariano R.L.R., S.J. Michereff, E.B. Silveira, S.M.P. Assis and A. Reis. 1997. Plant growth-promoting rhizobacteria in Brazil, p. 22-29. In: A. Ogoshi, K. Kabayashi, Y. Homma, F. Kodama, N. Kondo, S. Akino (eds), Plant Growth-Promoting Rhizobacteria. Present Status and Future Prospects. Sapporo, Japan.
• Mazzola M., D.K. Fujimo to, L.S. Thomashow and R.J. Cook. 1995. Variation in sensitivity of Gaeumannomyces graminis to antibiotics produced by fluorescent Pseudomonas spp. and effect on biological control of take-all of wheat. Appl. Environ. Microbiol. 61: 2554-2559.
• Mróz A., S. Martyniuk and J. Kuś. 1994. Response of winter wheat to seed applied microorganisms. Phytopathol. Pol. 7: 15-20.
• Paszkowski W.L., D. Masiak and J. Kobus. 1996. HCN biosynthesis by wheat rhizosphere bacteria (in Polish), p. 79-85. In: Teoretyczne i praktyczne aspekty allelopatii, IUNG, Puławy, Poland.
• Paszkowski W.L. 1998. The effect of HCN excreted by Pseudomonas fluorescens on the growth of some phytopathogenic fungi (in Polish). Zesz. Nauk. Akad. Roln. we Wrocławiu. Konferencje 332: 85-92.
• Pierson E.A. and D.M. Weller. 1994. Use of mixtures of fluorescent pseudomonads to suppress take-all and improve the growth of wheat. Phytopathology 84: 940-947.
• Ross I.L. and M.H. Ryder. 1994. Hydrogen cyanide production by a biocontrol strain of Pseudomonas corrugata: evidence that cyanide antagonises the take-all fungus in vitro, p. 131-133. In: M.H. Ryder, P.M. Stephens and G.D. Bowen (eds). Improving plant productivity with rhizosphere bacteria, Adelaide, South Australia.
• Ryder M.H., Z. Yan, T.E. Terrace, A.D. Rovira, W. Tang and R.L. Correll. 1999. Use of strains of Bacillus isolated in China to suppress take-all and rhizoctonia root rot, and promote seedling growth of glasshouse-grown wheat in Australian soils. Soil Biol. Biochem. 31: 19-29.
• Sarniguet A., P. Lucas and M. Lucas. 1992. Relationships between take-all, soil conduciveness to the disease, populations of fluorescent pseudomonads and nitrogen fertilizers. Plant Soil 145: 17-27.
• Strzelczyk E., K. Gorlach and H. Różycki. 1990 Chitinolytic and proteolytic activity of streptomyces isolated from root-free soil, rhizosphere and mycorhizosphere of pine (Pinus silvestris L.). Biol Fertil. Soils 9: 268-272.
• Sung K.C. and YR. Chung. 1997. Enhanced suppression of rice sheath blight using combination of bacteria which produce chitinases or antibiotics, p. 370-372. In: A. Ogoshi, K. Kobayashi, Y. Homma, F. Kodama, N. Kondo, S. Akino (eds). Plant Growth-Promoting Rhizobacteria. Present Status and Future Prospects. Sapporo, Japan.
• Tazawa-Isogami J., K. Watanabe, H. Yoshida, M. Sato and Y. Homma. 1997. Mutants of Pseudomonas fluorescens W8a deficient in antifungal compounds induced and their suppression of wheat take-all disease, p. 333-335. In: A. Ogoshi, K. Kabayashi, Y. Homma, F. Kodama, N. Kondo, S. Akino (eds), Plant Growth-Promoting Rhizobacteria. Present Status and Future Prospects. Sapporo, Japan.
• Tsuchiya K. 1997. Current status and future prospects in research and practice of biological control of plant diseases in Japan, p. 30-35. In: A. Ogoshi, K. Kabayashi, Y. Homma, F. Kodama, N. Kondo, S. Akino (eds), Plant Growth-Promoting Rhizobacteria. Present Status and Future Prospects. Sapporo, Japan.
• Weller D.M. 1988. Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Ann. Rev. Phytopathol. 26: 379-407.
• Weller D.M., J.M. Raaijmakers and L.S. Thomashow. 1997. The rhizosphere ecology of antibiotic-producing pseudomonads and their role in take-all decline, p. 58-64. In: A. Ogoshi, K. Kabayashi, Y. Homma, F. Kodama, N. Kondo, S. Akino (eds), Plant Growth-Promoting Rhizobacteria. Present Status and Future Prospects. Sapporo, Japan. Wenhua T. and Y. Hetong. 1997. Research and application of biocontrol of plant diseases and PGPR in China, p. 2-9. In: A. Ogoshi, K. Kabayashi, Y. Homma, F. Kodama, N. Kondo, S. Akino (eds), Plant Growth-Promoting Rhizobacteria. Present Status and Future Prospects. Sapporo, Japan.
• Wong P.T.W. 1994. Biocontrol of wheat take-all in the field using soil bacteria and fungi, p. 24-28. In: M.H. Ryder, P.M. Stephens and G.D. Bowen (eds), Improving plant productivity with rhizosphere bacteria, Adelaide, South Australia.
• Wong P.T.W. and R. Baker. 1984. Suppression of wheat take-all and ophiobolus patch by fluorescent pseudomonads from a Fusarium-suppresive soil. Soil Biol. Biochem. 16: 397-403.
• Wütrich B. and G. Défago. 1990. Suppression of wheat take-all and black root rot of tobacco by Pseudomonas fluorescens strain CHA0: results of field and pot experiments, p. 17-22. In: C. Keel, B. Koller and G. Défago (eds), Plant Growth-Promoting Rhizobacteria. Progress and Prospects. Interlaken, Switzerland.