Induction of somatic embryogenesis in spruce: Picea omorika, P. pungens "Glauca", P. breweriana and P. abies

• Autorzy: Hazubska T , Szczygiel K.
• Języki publikacji: EN

Abstrakty

EN

The studies were carried out on seed explants (mature zygotic embryos) of four spruce species: Picea omorika (Pancić) Purk., P. pungens ‘Glauca' Beisnn., P. breweriana S. Watson and P. abies (L.) Karst. The explants required darkness during the induction of embryogenic tissue. Temporary exposure of explants to light did not increase their embryogenic capacity. It was observed that the temperature of 25°C was the optimal for embryogenic tissue induction from mature zygotic embryos of Picea omorika. Cold treatment at 4°C during one week improved embryogenic tissue initiation in the explants of P. pungens ‘Glauca'. Seed explants of Picea omorika produced embryogenic tissue on medium lacking glutamine. Only explants of Picea abies and P. omorika showed embryogenic tissue formation. For effective proliferation of embryogenic tissue in Picea abies and P. omorika it was necessary to supply the medium with 2,4-D (4.5-9 μM) and BA (2.25 μM). Two of three analized lines showed that ABA promote somatic embryo maturation, at the ABA concentration 20 μM in the medium.

Słowa kluczowe

EN

Norway spruce; Picea pungens; Serbian spruce; Picea abies; blue spruce; Picea omorika; clonal propagation; spruce; somatic embryogenesis; maturation; embryogenesis; Picea breweriana; somatic embryo

• Wydawca: -
• Czasopismo: Dendrobiology
• Rocznik: 2003
• Tom: 50
• Opis fizyczny: p.17-24,fig.,ref.

Twórcy

autor

Hazubska T

• Polish Academy of Sciences, Parkowa 5, 62-035 Kornik, Poland

autor

Szczygiel K.

Bibliografia

• Afele J.C., Senaratna T., Mc Kersie B.D., Saxena P.K. 1992. Somatic embryogenesis and plant regeneration from zygotic embryo culture in blue spruce (Picea pungens Engelman.). Plant Cell Reports 11: 299–303.
• Arnold von S. 1987. Improved efficiency of somatic embryogenesis in mature embryos of Picea abies (L.) Karst. Journal of Plant Physiology 128: 233–244.
• Arnold von S., Egertsdotter U., Ekberg I., Gupta P., Mo H., Norgaard J. 1995. Somatic embryogenesis in Norway spruce (Picea abies). Somatic Embryogenesis in Woody Plants Kluwer. Academic Publishers 3: 17–36.
• Becwar M.R., Noland T.L., Wann S.R. 1987. Somatic embryo development and plant regeneration from embryogenic Norway spruce callus. Tappi Journal 70: 155–160.
• Bellarosa R., Mo L.H., von Arnold S. 1992. The influence of auxin and cytokinin on proliferation and morphology of somatic embryos of Picea abies (L.) Karst. Annals of Botany 70: 199–206.
• Bozhkov P.V., Arnold von S. 1998. Polyethylene glycol promotes maturation but inhibits further development of Picea abies somatic embryos. Physiologia Plantarum 104: 211–224.
• Bozhkov P.V., Lebedenko L.A., Shiryaeva G.A. 1992. A pronounced synergistic effect of abscisic acid and 6-benzyladenine on Norway spruce (Picea abies L. Karst) somatic embryo maturation. Plant Cell Reports 11: 386–389.
• Budimir S., Vujicic R. 1992. Benzyladenine induction of buds and somatic embryogenesis in Picea omorika (Pancic) Purk. Plant Cell, Tissue and Organ Culture 31: 89–94.
• Chalupa V. 1985. Somatic embryogenesis and plantlet regeneration from cultured immature and mature embryos of Picea abies (L.) Karst. Communicationes Instituti Forestralis 14: 57–63.
• Cheliak W.M., Klimaszewska K. 1991. Genetic variation in somatic embryogenic response in open-pollinated families of black spruce. Theoretical and Applied Genetics 82: 185–190.
• Dunstan D.I., Tautorus T.E., Thorpe T.A. 1995. Somatic Embryogenesis in Woody Plants. In: In Vitro Embryogenesis in Plants. Kluwer Academic Publishers, Dordrecht: 471–538.
• Gupta P.K., Grob J.A. 1995. Somatic embryogenesis in conifers. In: Jain S., Gupta P., Newton R. (eds), Somatic Embryogenesis in Woody Plants. Kluwer Academic Publishers, Dordrecht 1: 81–98.
• Gupta P.K., Durzan D.J. 1986. Plantlet regeneration via somatic embryogenesis from subcultured callus of mature embryos of Picea abies (Norway spruce). In Vitro Cellular and Developmental Biology – Plant 22: 685–688.
• Hakman I., Fowke L.C., von Arnold S., Eriksson T. 1985. The development of somatic embryos in tissue cultures initiated from immature embryos of Picea abies (Norway spruce). Plant Science 38: 53–59.
• Harvengt L., Trontin J.F., Reymont I., Canlet F., Paques M. 2001. Molecular evidence of true- totype propagation of 3-year-old Norway spruce through somatic embryogenesis. Planta 213: 823–832.
• Hogberg K.A., Bozhkov P.V., Gronnroos R.von Arnold S. 2001. Critical factors affecting ex vitro performance of somatic embryo plants of Picea abies. Scandinavian Journal of Forest Research 16: 259–304.
• Hristoforoglu K., Schmidt J., Bolhar-Nordenkampf H. 1995. Development and germination of Abies alba somatic embryos. Plant Cell, Tissue and Organ Culture 40: 277–284.
• Jalonen P., von Arnold S. 1991. Characterization of embryogenic cell lines of Picea abies in relation to their competence for maturation. Plant Cell Reports 10: 384–387.
• Jain S. M., Newton R.J., Soltes E.J. 1988. Enhacement of somatic embryogenesis in Norway spruce (Picea abies L.). Theoretical and Applied Genetics 76: 501–506.
• Khlifi S., Tremblay F.M. 1995. Maturation of black spruce somatic embryos. I Effect of L-glutamine on the number and germinability of somatic embryos. Plant Cell, Tissue Organ Culture 41: 23–32.
• Klimaszewska K., Park Y-S., Overton C., Maceacheron I., Bonga J.M. 2001. Optimized somatic embryogenesis in Pinus strobus L. In Vitro Cellular and Developmental Biology- Plant 37: 392–399.
• Leustek T., Kirby E.G. 1988. The influence of glutamine on growth and viability of cell suspension cultures of Douglas – fir after exposure to ethylene glycol. Tree Physiology 4: 371–380.
• Misra S., Attree S.M., Leal I., Fowke L.C. 1993. Effect of abscisic acid, osmoticum and dessication on synthesis of storage proteins during the development of white spruce somatic embryos. Annals of Botany 71: 11–22.
• Misra S. 1994. Conifer zygotic embryogenesis, somatic embryogenesis and seed germination: Biochemical and molecular advances. Seed Science Reseach 4: 357–384.
• Mo L.H., von Arnold S. 1991. Origin and development of embryogenic cultures from seedlings of Norway spruce (Picea abies). Journal of Plant Physiology 138: 223–230.
• Norgaard J.V., Krogstrup P. 1991. Cytokinin induced somatic embryogenesis from immature embryos of Abies nordmanniana Lk. Plant Cell Reports 9: 509–513.
• Park Y.S., Barret J.D., Bonga J.M. 1998. Application of somatic embryogenesis in high-value clonal forestry: deployment, genetic control, and stability of cryopreserved clones. In Vitro Cellular and Developmental Biology – Plant 34: 231–239.
• Roberts D.R. 1991. Abscisic acid and mannitol promote early development, maturation and storage protein accumulation in somatic embryos of interior spruce. Physiologia Plantarum 83: 247–254.
• Ruaud J.N., Bercetche J., Paques M. 1992. First evidence of somatic embryogenesis from needles of one-year-old Picea abies plants. Plant Cell Reports 11: 563–566.
• Ruaud J.N. 1993. Maturation and conversion into plantlets of somatic embryos derived from needles and cotyledons of 7–56-day old Picea abies. Plant Science 92: 213–220.
• Seneta W., Dolatowski J. 1997. Dendrologia PWN. Warszawa.
• Vujicic R., Budimir S. 1995. Somatic embryogenesis and plant regeneration in Picea omorika. Somatic Embryogenesis in Woody Plants. Kluwer Academic Publishers Netherlands 3: 81–97.