RAS inhibitors decrease apoptosis of acinar cells and increase elimination of pancreatic stellate cells after in the course of experimental chronic pancreatitis induced by dibutyltin dichloride

• Autorzy: Madro A. , Korolczuk A. , Czechowska G. , Celinski K. , Slomka M. , Prozorow-Krol B. , Korobowicz E.
• Języki publikacji: EN

Abstrakty

EN

Chronic pancreatitis (CP) is a progressive disease, in which the exocrine function of the gland is gradually lost and fibrosis develops due to repeated episodes of acute pancreatitis. The aim of the study was to investigate the effects of RAS inhibitors on the apoptosis of acinar cells and pancreatic stellate cells (PSCs) elimination in experimental CP induced by dibutyltin dichloride (DBTC). CP was induced by administration of DBTC to the femoral vein. Simultaneously captopril, losartan, enalapril and lisinopril were administered intraperitoneally. The rats were decapitated after 60 days and tissue of pancreas was collected. In rats treated by DBTC the features of inflammatory infiltration, ductal lumen dilatation, fibrosis were found. Strong reactivity with capsase2L and clusterin-ß antibodies was observed in areas of fibrosis. In animals treated with RAS inhibitors inflammatory changes and fibrosis were less severe. In groups of rats treated with DBTC and RAS inhibitors immunoreactivity of capsase2L and clusterin-ß was weak. Positive immunostaining against smooth muscle actine and desmin was observed in the elongated cells (PSC-s). This reaction was weak in groups of rat treated with DBTC and RAS inhibitors. Treatment of CP rats with RAS inhibitors alleviate apoptosis of pancreatic acinar cells and induces PSCs elimination.

Słowa kluczowe

EN

dibutyltin dichloride; acinar cell; chronic pancreatitis; renin-angiotensin system; progressive disease; exocrine function; apoptosis; pancreatic stellate cell; fibrosis; acute pancreatitis

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology. Supplement
• Rocznik: 2008
• Tom: 59
• Numer: 2
• Opis fizyczny: p.239-249,fig.,ref.

Twórcy

autor

Madro A.

• Medical University of Lublin, Jaczewski Street 8, 20-954 Lublin, Poland

autor

Korolczuk A.

autor

Czechowska G.

autor

Celinski K.

autor

Slomka M.

autor

Prozorow-Krol B.

autor

Korobowicz E.