The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X-100

• Autorzy: Panasiewicz M , Domek H. , Hoser G. , Fedoryszak N. , Kawalec M. , Pacuszka T.
• Języki publikacji: EN

Abstrakty

EN

Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We assessed the effect of the ceramide structure of four [3H]-labeled GM1 ganglioside molecular species (GM1s) taken up by HL-60 cells on their occurrence in LDMs, and compared it with our previous observations for DRMs. All GM1s contained C18 sphingosine, which was acetylated in GM1(18:1/2) or acylated with C14, C18 or C18:1 fatty acids (Fas)

Słowa kluczowe

EN

ceramide structure; ganglioside; low density membrane fraction; HL-60 cell; Triton X-100 detergent; membrane domain; myristic acid; sonication

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2009
• Tom: 14
• Numer: 2
• Opis fizyczny: p.175-189,fig.,ref.

Twórcy

autor

Panasiewicz M

• Medical Centre of Postgraduate Education, Marymoncka 99, 01-813 Warsaw, Poland

autor

Domek H.

autor

Hoser G.

autor

Fedoryszak N.

autor

Kawalec M.

autor

Pacuszka T.

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