Evaluation of the usefulness of PCR method for the detection of processed animal protein in feedingstuffs

• Autorzy: Weiner A , Kwiatek K.
• Języki publikacji: EN

Abstrakty

EN

The aim of this paper is to present the results of comparative evaluation of the usefulness of PCR and microscopic methods in the detection of processed animal protein in feedingstuffs. Out of 127 samples of feedingstuffs examined by microscopic method 82 (64.57%) were found to be positive. In comparing the results obtained by microscopic examination and PCR technique, it was found that both methods agreed in 67.7% giving 44 positive and 42 negative results. The rest results (32.3%) were not coincident. In the validation study, the limit of the detection for PCR was determined on 0.2% for beef and pork meat and bone meal (MBM) and 0.1% for poultry MBM. Analysing results received with the help of the microscopic method and PCR technique it is possible to state that the molecular biology methods can at present, be used as a supplementary method.

Słowa kluczowe

EN

polymerase chain reaction; animal protein; degenerative disease; feedstuff; bovine spongiform encephalopathy; detection; veterinary medicine; microscopic examination; animal disease

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2008
• Tom: 52
• Numer: 3
• Opis fizyczny: p.411-415,fig.,ref.

Twórcy

autor

Weiner A

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kwiatek K.

Bibliografia

• 1. Boom R., Sol C., Beld M., Weel J., Goudsmith J., Wertheim-van Dillen P.: Improved silica- guanidiniumthiocyanate DNA isolation procedure based on binding of bovine alpha-casein to silica particles. J Clin Microbiol 1999, 37, 615-619.
• 2. Bottero M.T., Civera T., Nucera D., Turi R.M.: Design of universal primers for the detection of animal tissues in feedstuff. Vet Res Commun 2003, 27, Suppl. 1, 667-669.
• 3. Bottero M.T., Dalmasso A., Nucera D., Turi R.M., Rosati S., Squadrone S., Goria M., Civera T.: Development of a PCR assay for the detection of animal tissues in ruminant feeds. J Food Prot 2003, 66, 2307-2312.
• 4. Buntjer J.B., Lenstra J.A.: Mammalian species identification by interspersed repeat PCR fingerprinting. J Ind Microbiol Biotech 1998, 21, 121-127.
• 5. Colgan S., O'Brien L., Maher M., Shilton N., McDonnel K., Ward S.: Development of a DNA-based assay for species identification in meat and bone meal. Food Res Int 2001, 34, 409-414.
• 6. Commision Decision of 29 December 2000 concerning control measures required for the implementation of Council Decision 2000/766/EC concerning certain protection measures with regard to transmissible spongiform encephalopathies and the feeding of animal protein (O.J.L 2/32, 05.01.2001).
• 7. Commission Directive 2003/126/EC of 23 December 2003 on the analytical method for the determination of constituents of animal origin for the official control of feedingstuffs (O.J.L 339/78, 24.12.2003).
• 8. Commission Regulation (EC) No. 1234/2003 of 10 July 2003 amending Annexes I, IV and XI to Regulation (EC) No. 999/2001 of the European Parliament and of the Council and Regulation (EC) No. 1326/2001 as regards transmissible spongiform encephalopathies and animal feeding (O.J.L 173, 11.07.2003).
• 9. Commission Regulation (EC) No. 1292/2005 of 5 August 2005 amending Annex IV to Regulation (EC) No. 999/2001 of the European Parliament and of the Council as regards animal nutrition (O.J.L 205/3, 6.8.2005).
• 10. Dalmasso A., Fontanella E., Piatti P., Civera T., Rosati S., Bottero M.T.: A multiplex PCR assay for the identification of animal species in feedstuffs. Mol Cell Probes 2004, 18, 81-87.
• 11. Engling F-P., Jörgensen J.S., Paradies-Severin I., Hahn H.: Evidence of animal meal in feeds. Kraftfutter/Feed Magazine 2000,1, 14-17.
• 12. Frick G., Roetschi A., Hauswirth H.: Mikroskopische Untersuchung von Futtermitteln. Agrarforschung 2002, 9, 497-504.
• 13. Frick G., Roetschi A., Hauswirth H.: Futtermittel: Prüfung der Zusammensetzung. Agrarforschung 2003, 10, I-VIII.
• 14. Gizzi G., von Hoist Ch.: Determination of processed animal proteins, including meat and bone meal, in animal feed. J AOAC Int 2004, 87, 1334-1341.
• 15. Gizzi G., von Hoist C., Baeten V., Berben G.: Raamsdonk L.W.D.: Intercomparison study for the determination of processed animal proteins including meat and bone meal in animal feed. Final report of 16 May 2003.
• 16. Gizzi G., Raamsdonk L.W.D., Baeten V., Murray I., Berben G., Brambilla G., von Holst C.: An overview of tests for animal tissues in feeds applied in response to public health concerns regarding bovine spongiform encephalopathy. Rev Sci Tech Off Int Epiz 2003, 22, 311- 331.
• 17. Gouli Z., Mingguang Z., Zhijiang Z., Hongsheng O., Qiang I.: Establishment and application of polymerase chain reaction for the identification of beef. Meat Sci 1999, 51, 233-236.
• 18. Hahn H.: Animal meal: production and determination in feedstuffs and the origin of bovine spongiform encephalopathy. Naturwissenschaften 1999, 86, 62-70.
• 19. Heidenreich E.: Zero tolerance or minimised residual risk? Kraftfutter/Feed Magazine 2001, 10, 367-371.
• 20. Hormish D.E.: Traceability of processed animal proteins with varying texture in feed: determination with microscopic and polymerase chain reaction methods. Biotechnol Agron Soc Environ 2004, 8, 257-266.
• 21. Kingombe C.I.B., Lüthi E., Schlosser H., Howald D., Kuhn M., Jemmi T.: A PCR-based test for species-specific determination of heat treatment conditions of animal meals as an effective prophylactic method for bovine spongiform encephalopathy. Meat Sci 2001, 57, 35-41.
• 22. Krčmàř P., Renčovà E.: Identification of bovine-specific DNA in feedstuffs. J Food Prot 2001, 64, 117-119.
• 23. Kwiatek K., Weiner A., Przeniosło M.: Two-year study on prevalence of processed animal proteins in feedingstuffs. Bull Vet Inst Pulawy 2007, 51, 603-607.
• 24. Lahiff S., Glennon M., O'Brien L., Lyng J., Smith T., Maher M., Shilton N.: Species-specific PCR for the identification of ovine, porcine and chicken species in meat and bone meal (MBM). Mol Cell Probes 2001, 15, 27-35.
• 25. Pinotti L., Campagnoli A., Tongon G., Cheli F., DeH'Orto V., Savoini G.: Microscopic method in processed animal proteins identification in feed: applications of image analysis. Biotechnol Agron Soc Environ 2004, 8, 249-251.
• 26. Prado M., Casqueiro J., Iglesias Y., Cepeda A., Barros- Veláquez J.: Application of polymerase chain reaction (PCR) method as a complementary tool to microscopic analysis for the detection of bones and other animal tissues in home-made animal meals. J Sci Food Agric 2004, 84, 505-512.
• 27. Raamsdonk L.W.D., Vancustem J., Zegers J., Frick G., Jorgenson J.S., Pinckaers V., Bosch J., Paradies-Severin I.: The microscopic detection of animal proteins in feeds. Biotechnol Agron Soc Environ 2004, 8, 241-247.
• 28. Regulation (EC) No. 1774/2002 of the European Parliament and of the Council of 3 October 2002 laying down health rules concerning animal by-poducts not intendend for human consumption (O.J.L. 273, 10.10.2002).
• 29. Regulation (EC) No. 999/2001 of the European Parliament of the Council of 22 May 2001 laying down rules for the prevention, control and eradication of certain transmissible spongiform encephalopathies (O.J.L. 147, 31.05.2001).
• 30. Regulation of the Minister of Agriculture and Rural Development (Poland) of March 6, 2006 on methodology of analysis for the determination of nutritive components and feed additives in feed materials, premixes, compound feedingstuffs, and medicated feedingstuffs. Law Gazette No. 54, item 389.
• 31. Saiki R.K., Gelfand D.H., Stoffel S.: Primer directed enzymatic amplification of DNA with a termostable DNA polymerase. Science 1988, 239, 487-491.
• 32. Tartaglia M., Saulle E., Pestalozza S., Morelli L., Antonucci G., Battaglia P.A.: Detection of bovine mitochondrial DNA in ruminant feeds: a molecular to test for the presence of bovine-derived materials. J Food Prot 1998, 5, 513-518.
• 33. Unseld M., Beyermann B., Brant P., Hiesel R.: Identification of species origin of highly processed meat products by mitochondrial DNA sequences. PCR Methods Appl 1995, 4, 241-243.
• 34. von Holst C., Baeten V., Berben G., Brambilla G.: Overview of methods for the detection of species specific proteins in feed intended for farmed animals. European Commission. IRMM. September 2004.
• 35. Wolf H., Gaede W., Wolf C., Zellermann S., Höber S.: Nachweis von tierischen Bestandteilen im Mischfutter. Vergleichende Untersuchungen. Fleischwirtschaft 2001, 5, 197-201.
• 36. Żmudziński J.F., Polak M. P., Rożek M., Larska M.: BSE - myths and facts: Is Poland facing BSE epidemic? Conference materials, Puławy 2002, pp. 7-28.