Flow cytometric analysis of leukocytes in porcine mammary secretions

• Autorzy: Pomorska-Mol M , Markowska-Daniel I. , Bednarek D.
• Języki publikacji: EN

Abstrakty

EN

Nine sows were monitored for leukocyte subsets in mammary secretions during lactation. Using flow cytometry, percentages of monocytes/macrophages (CD45⁺CD14⁺), T cells (CD3⁺), and their subpopulations (CD4⁺, CD8⁺ and double positive (DP) CD4⁺CD8⁺), and B cells (CD21⁺) in colostrum and milk were determined. The mean percentage of CD3⁺cells was relatively stable throughout lactation. About 40% of colostral lymphocytes were identified as CD3⁺, while in milk they comprised from 24% to 39%. The percentage of both CD8⁺ and CD4⁺ cells were relatively stable during lactation, beside significant decrease at the last day of lactation. Double positive cells comprised a small percentage of cells and did not exceed 7.5%. The frequency of DP lymphocytes increased significantly 6 h after parturition, and then remained relatively stable until 20th d of lactation, followed by a significant decrease at day 28 postpartum. The CD8⁺ cells prevailed over CD4⁺ cells throughout whole lactation. The CD4/CD8 ratio was similar during lactation period and ranged from 0.50 to 0.53 at the beginning and the end of lactation, respectively. Lymphocytes B represented about 20% of all lymphocytes of mammary secretions. CD45⁺CD14⁺ cells ranged between 5.01 and 10.14% of all leukocytes.

Słowa kluczowe

PL

sow; colostrum; milk; leucocyte; flow cytometry; mammary secretion; porcine mammary secretion; lactation

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 2
• Opis fizyczny: p.188-192,fig.,ref.

Twórcy

autor

Pomorska-Mol M

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Markowska-Daniel I.

autor

Bednarek D.

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