Abscisic acid does not influence the subcellular distribution of the HYL1 protein from Arabidopsis thaliana

• Autorzy: Lesicka-Gorecka J. , Szarzynska B. , Sawczak M. , Bagdiul I. , Gorski P. , Jarmolowski A. , Szweykowska-Kulinska Z.
• Języki publikacji: EN

Abstrakty

EN

HYL1 is a nuclear protein involved in the processing of miRNAs but its exact function remains unknown. Arabidopsis thalianahyl1mutants exhibit hypersensitivity to ABA. We decided to answer the question whether ABA affects the HYL1 protein localization within the cell and show that it does not. We also studied the expression of HYL1 in different tissues and organs. In this paper we show for the first time the expression profile of the HYL1 protein using anti-HYL1 antibodies. The protein is present in seedlings and mature plants in all organs studied, with the highest amount in inflorescences. A. thalianaHYL1 protein has several repetitions of a 28-amino-acid sequence at the C-terminus that confer protein instability. Our bioinformatic analysis of HYL1 homologs in different Brassicaspecies shows that this repetition is typical only for Arabidopsis. This may suggest a relatively late evolutionary acquisition of the C-terminal domain.

Słowa kluczowe

PL

abscisic acid; subcellular distribution; HYL1 gene; protein; HYL1 protein; Arabidopsis thaliana; expression profile; nuclear protein; anti-HYL1 antibody; mature plant; Brassica

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2008
• Tom: 55
• Numer: 3
• Opis fizyczny: p.517-524,fig.,ref.

Twórcy

autor

Lesicka-Gorecka J.

• Adam Mickiewicz University, Miedzychodzka 5, 60-371 Poznan, Poland

autor

Szarzynska B.

autor

Sawczak M.

autor

Bagdiul I.

autor

Gorski P.

autor

Jarmolowski A.

autor

Szweykowska-Kulinska Z.

Bibliografia

• Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389-3402.
• Clamp M, Cuff J, Searle SM, Barton GJ (2004) The Jalview Java Alignment Editor. Bioinformatics 20:426-427.
• Dobson CM (1999) Protein misfolding, evolution and disease. Trends Biochem Sci 24:329-332.
• Fujioka Y, Utsumi M, Ohba Y, Watanabe Y (2007) Location of a possible miRNA processing site in SmD3/SmB nuclear bodies in Arabidopsis. Plant Cell Physiol 48:1243-1253.
• Han M-H, Goud S, Song L, Fedoroff N (2004) The Arabidopsisdouble-stranded RNA-binding protein HYL1 plays a role in microRNA-mediated gene regulation. Proc Natl Acad Sci USA 101:1093-1098.
• Hiraguri A, Itoh R, Kondo N, Nomura Y, Aizawa D, Murai Y, Koiwa H, Seki M, Shinozaki K, Fukuhara T (2005) Specific interactions between Dicer-like proteins and HYL1/DRB-family dsRNA-binding proteins in Arabidopsis thaliana. Plant Mol Biol 57:173-188.
• Hugouvieux V, Kwak JM, Schroeder JI (2001) An mRNA cap binding protein, ABH1, modulates early abscisic acid signal transduction in Arabidopsis. Cell 106:477-487.
• Iakoucheva LM, Radivojac P, Brown CJ, O'Connor TR, Sikes JG, Obradovic Z, Dunker AK (2004) The importance of intrinsic disorder for protein phosphorylation. Nucleic Acids Res 32:1037-1049.
• Kurihara Y, Takashi Y, Watanabe Y (2006) The interaction between DCL1 and HYL1 is important for efficient and precise processing of pri-miRNA in plant micro-RNA biogenesis. RNA 12:206-212.
• Lesicka J, Piontek P, Szweykowska-Kulinska Z, Jarmolowski A (2004) siRNAs and miRNAs: small RNA molecules for big tasks. Acta Physiol Plant 26:363-368.
• Li CF, Pontes O, El-Shami M, Henderson IR, Bernatavichute YV, Chan SW, Lagrange T, Pikaard CS, Jacobsen SE (2006) An ARGONAUTE4-containing nuclear processing center colocalized with cajal bodies in Arabidopsis thaliana. Cell 126:93-106.
• Li J, Kinoshita T, Pandey S, Ng CK, Gygi SP, Shimazaki K, Assmann SM (2002) Modulation of an RNA-binding protein by abscisic-acid-activated protein kinase. Nature 418:793-797.
• Li X, Romero P, Rani M, Dunker AK, Obradovic Z (1999) Predicting protein disorder for N-, C-, and internal regions. Genome Informatics 10:30-40.
• Lu C, Fedoroff N (2000) A mutation in the ArabidopsisHYL1 gene encoding a dsRNA binding protein affects responses to abscisic acid, auxin and cytokinin. Plant Cell 12:2351-2365.
• Notredame C, Higgins D, Heringa J (2000) T-Coffee: A novel method for multiple sequence alignments. J Mol Biol 302:205-217.
• Prols M, Topfer R, Schell J, Steinbiss HH (1988) Transient gene expression in tobacco protoplasts. Plant Cell Rep 7:221-224.
• Radivojac P, Iakocheva LM, Oldfield CJ, Obradovic Z, Uversky VN, Dunker AK (2007) Intrinsic disorder and functional proteomics. Biophys J 92:1439-1456.
• Riera M, Redko Y, Leung J (2006) ArabidopsisRNA-binding protein UBA2a relocalizes into nuclear speckles in response to abscisic acid. FEBS Lett 580:4160-4165.
• Romero P, Obradovic Z, Dunker AK (1997) Sequence data analysis for long disordered regions prediction in the calcineurin family. Genome Informatics 8:110-124.
• Romero P, Obradovic Z, Li X, Garner E, Brown C, Dunker AK (2001) Sequence complexity of disordered protein. Proteins 42:38-48.
• Song L, Han M-H, Lesicka J, Fedoroff N (2007) Arabidopsisprimary microRNA processing proteins HYL1 and DCL1 define a nuclear body distinct from the Cajal body. Proc Natl Acad Sci USA 104:5437-5442.
• Schaffer AA, Aravind L, Madden TL, Shavirin S, Spouge JL, Wolf YI, Koonin EV, Altschul SF (2001) Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements. Nucleic Acids Res 29:2994-3005.
• Sunkar R, Chinnusamy V, Zhu J, Zhu JK (2007) Small RNAs as big players in plant abiotic stress responses and nutrient deprivation. Trends Plant Sci 12:301-309.
• St Johnston D, Brown NH, Gall JG, Jantsch M (1992) A conserved double-stranded RNA-binding domain. Proc Natl Acad Sci USA 89:10979-10983.
• Szweykowska-Kulinska Z, Jarmolowski A, Figlerowicz M (2003) RNA interference and its role in the regulation of eucaryotic gene expression. Acta Biochim Polon 50:217-229.
• Vazquez F, Gasciolli V, Crete P, Vaucheret H (2004) The nuclear dsRNA binding protein HYL1 is required for microRNA accumulation and plant development, but not posttranscriptional transgene silencing. Curr Biol 14:346-351.
• Verslues PE, Guo Y, Dong CH, Ma W, Zhu JK (2006) Mutation of SAD2, an importin β-domain protein in Arabidopsis, alters abscisic acid sensitivity. Plant J 47:776-787.
• Wu F, Yu L, Cao W, Mao Y, Liu Z, He Y (2007) The N-terminal double-stranded RNA binding domains of Arabidopsis HYPONASTIC LEAVES1 are sufficient for pre-microRNA processing. Plant Cell 19:914-925.
• Xiong L, Schumaker KS, Zhu JK (2002) Cell signaling during cold, drought, and salt stress. Plant Cell14 (Suppl): S165-S183.
• Xiong L, Gong Z., Rock CD, Subramanian S, Guo Y, Xu W, Galbraith D, Zhu JK (2001) Modulation of abscisic acid signal transduction and biosynthesis by an Sm-like protein in Arabidopsis. Dev Cell 1:771-781.
• Yang L, Liu Z, Lu F, Dong A, Huang H (2006) SERRATE is a novel nuclear regulator in primary microRNA processing in Arabidopsis. Plant J 47:841-850.