pVAX1 plasmid vector-mediated gene transfer of soluble TRAIL suppresses human hepatocellular carcinoma growth in nude mice

• Autorzy: Zhang Y. , Ma C.H. , Liu H. , Zhang X.M. , Sun W.S.
• Języki publikacji: EN

Abstrakty

EN

The extracellular domain of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) may function as a soluble cytokine to selectively kill various cancer cells without toxicity to most normal cells. We used a high-biosafety plasmid pVAX1 as a vector and constructed a recombinant plasmid expressing the extracellular domain (95-281 aa) of human TRAIL fused with signal peptides of human IgGγ, designated as pVAX-sT. Transduction of human BEL7402 liver cancer cells with pVAX-sT led to high levels of sTRAIL protein in the cell culture media and induced apoptosis. The therapeutic potential of pVAX-sT was then evaluated in the BEL7402 transplanted naked mouse model. Subsequent intratumoral administration of naked pVAX-sT resulted in the expression of soluble TRAIL in the sera and the tumor site, as well as effective suppression of tumor growth, with no toxicity to liver. In conclusion, the successful inhibition of liver cancer growth and the absence of detectable toxicity suggest that pVAX-sT could be useful in the gene therapy of liver cancer.

Słowa kluczowe

EN

gene therapy; tumour necrosis factor-related apoptosis-inducing ligand; cancer cell; hepatocellular carcinoma; liver; pVAX1 plasmid; apoptosis; normal cell

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2007
• Tom: 54
• Numer: 2
• Opis fizyczny: p.307-313,fig.,ref.

Twórcy

autor

Zhang Y.

• Medical School of Shandong University, Jinan, China

autor

Ma C.H.

autor

Liu H.

autor

Zhang X.M.

autor

Sun W.S.

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