Wykrywanie genow smr i qacA u szczepow Staphylococcus aureus z zastosowaniem metody PCR

• Autorzy: Stefanska J. , Mlynarczyk G. , Mlynarczyk A. , Starosciak B. , Luczak M.
• Języki publikacji: PL

Abstrakty

PL

U 31 szczepów Staphylococcus aureus, badano obecność genów smr i qacA przy zastosowaniu metody PCR. Badaniami objęto 22 szczepy MRSA (w tym 20 opornych na czwartorzędowe sole amoniowe) oraz 9 szczepów MSSA.

EN

The 31 strains of Staphylococcus aureus were examined for the presence of smr and qacA determinants. The smr gene was found in 15 strains. Fourteen of them were MRSA resistant to quaternary ammonium compounds, ethidium bromide, and acriflavine. One was MSSA strain resistant to ethidium bromide and acriflavine. The qacA gene was found in two MRSA strains resistant to quaternary ammonium compounds, ethidium bromide, chlorhexidine andacriflavine. One of these two strains possessed both smr and qacA genes.

Słowa kluczowe

PL

srodki antyseptyczne; szczepy bakteryjne; gen qacA; srodki dezynfekcyjne; lancuchowa reakcja polimerazy; opornosc na sole amoniowe; gen smr; Staphylococcus aureus

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2002
• Tom: 54
• Numer: 3
• Opis fizyczny: s.183-189,rys.,tab.,bibliogr.

Twórcy

autor

Stefanska J.

• Akademia Medyczna, Warszawa

autor

Mlynarczyk G.

autor

Mlynarczyk A.

autor

Starosciak B.

autor

Luczak M.

Bibliografia

• 1. Berg T, Firth N, Apisiridej S i inni. Complete nucleotide sequence of pSK41: evolution of staphylococcal conjugative multiresistance plasmids J Bacteriol 1998; 180; 4350-59.
• 2. Bjorland J, Sunde M, Waage S. Plasmid-borne smr gene causes resistance to quaternary ammonium compounds in bovine Staphylococcus aureus. J Clin Microbiol 2001; 39: 3999-4004.
• 3. Heir L. Sundheim G, Holek AL. The Staphylococcus qacH gene product: a new member of SMR family encoding multidrug resistance. FEMS Microbiol Lett 1998; 163: 49-56.
• 4. Jeljaszewicz J, Młynarczyk G, Młynarczyk A. Present and future problems of resistance in Gram-positive cocci. Infection 1998, 26; 1-6.
• 5. Kazama H, Hamashima H, Sasatsu M. Arai T. Distribution of the antiseptic-resistance gene qacEΔ1 in gram-positive bacteria. FEMS Microbiol Lett 1998; 165; 295-99.
• 6. Kuroda M, Ohta T, Uchiyama l i inni Whole genome sequencing of methicillin-resistant Staphylococcus aureus, the major hospital pathogen. Lancet 2001; 357; 1225-40
• 7. Littlejohn TG, Rouch DA, Cram DS i inni. Molecular and sequence analysis of qacA specifying antiseptic/disinfectant resistance in staphylococci. W: Molecular Biology of the Staphylococci, Red. RP Novick. VCH Publishers Inc.; New York 1990; pp. 175-80.
• 8. Littlejohn TG, Paulsen IT, Gillepsie MT i inni. Substrate specificity and energetics of antiseptic and disinfectant resistance in Staphylococcus aureus. FEMS Microbiol Lett 1992; 95: 259-66.
• 9. Mayer S, Boos M, Beyer A i inni. Distribution of the antiseptic resistance genes qacA,qacB and qacC in 497 methicillin-resistant and susceptible European isolates of Staphylococcus aureus. J Antimicrob Chemother 2001; 47; 896-97.
• 10. McDonnel G, Russell AD. Antiseptics and disinfectants: activity, action and resistance. Clin Microbiol Rev 1999; 12: 147-79.
• 11. Młynarczyk A, Młynarczyk G, Jeljaszewicz J. The genome of Staphylococcus aureus: a review. Zentbl Bakteriol 1998; 287: 277-314.
• 12. National Committee for Clinical Laboratory Standards. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. M7-A3. NCCLS, Wayne, Pa 1998.
• 13. Noguchi N, Hase M, Kitta M i inni. Antiseptic susceptibility and distribution of antiseptic-resistance genes in methicillin-resistant Staphylococcus aureus. FEMS Microbiol Lett 1999; 172; 247-53.
• 14. Paulsen IT, Skurray RA, Tam R i inni. The SMR family: a novel family of multidrug efflux proteins involved with the efflux of lipophilic drugs. Mol Microbiol 1996; 19: 1167-75.
• 15. Paulsen IT, Brown MH, Skurray RA: Characterization of the earliest known Staphylococcus aureus plasmid encoding a multidrug efflux system. J Bacteriol 1998; 180: 3477-79.
• 16. Rodgers DJ, McCullagh JJ, McNamee PT i inni. An investigation into the efficacy of hatchery disinfectants against strains of Staphylococcus aureus associated with the poultry industry. Vet Microbiol 2001; 82: 131-40.
• 17. Rouch DA, Cram DS., DiBerardino D i inni. Efflux-mediated antiseptic resistance gene qacA from Staphylococcus aureus: common ancestry with tetracycline-and sugar-transport proteins. Mol Microbiol 1990, 4: 2051-62.
• 18. Sasatsu M, Shibata Y, Noguchi N, Kona M. High-level resistance to ethidium bromide and antiseptics in Staphylococcus aureus. FEMS Microbiol Lett 1992; 93; 109-14.
• 19. Stefańska J. Substancje czynne środków dezynfekcyjnych - mechanizmy działania, oporność drobnoustrojów. Mikrobiologia Medycyna 2000; 1; 17-24.
• 20. Stefańska J, Młynarczyk G, Młynarczyk A i inni. Oporność szczepów Staphylococcus aureus na IV-rzędowe sole amoniowe i chlorheksydynę. Med Dośw Mikrobiol 2002; 54: 191-97.