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1993 | 40 | 3 |

Tytuł artykułu

Subcellular localization of two different type-1 casein kinases from yeast

Warianty tytułu

Języki publikacji

EN

Abstrakty

Specific antibodies directed against the two yeast type-1 casein kinases (CK1) were used to study the localization of both 45 kDa and 27 kDa casein kinase species in yeast cells by immunofluorescence. Our results indicate that the larger and smaller CK1 species are localised in different compartments of the yeast cell. The 45 kDa enzyme is present in the cytoplasm of the cell both during the logarithmic and stationary growth phase. The 27 kDa CK1 was found in the nucleus in the cells in logarithmic growth phase while the enzyme from the stationary phase was present in the cytoplasm. Our results suggest that the 27 kDa casein kinase may play some role in yeast cell division control by displacement from the nucleus to the cytoplasm.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

40

Numer

3

Opis fizyczny

p.405-410,fig.

Twórcy

autor
  • Uniwersytet M.Curie-Sklodowskiej, Akademicka 19, 20-033 Lublin, Poland
autor
autor

Bibliografia

  • 1. Tuazon, P.T. & Traught, J.A. (1991) Casein kinase I and II - mu I ti potential serine protein kinases: structure, function and regulation. Adv. Second Messetiger Phosphoprotein Res. 23,123 -164.
  • 2. Dahmus, M.E. (1981) Purification and properties of calf thymus casein kinases I and II. J. Biol. Chem. 256,3319 - 3325.
  • 3. Hathaway, G.M. & Traugh, J.A. (1979) Cyclic nucleotidc-independcnt protein kinases from rabbit reticulocytes. Purification of casein kinases. /. Biol. Chem. 254, 762 - 768.
  • 4. Kudlicki, W., Grankowski, N. & Gąsior, E. (1978) Isolation and properties of two protein kinases from yeast which phosphorylate casein and some ribosomal proteins. Eur. J. Biochem. 84,493 -498.
  • 5. Szyszka, R., Kudlicki, W., Grankowski, N. & Gąsior, E. (1985) A minor species of a type I casein kinase from yeast phosphorylating threonine residues of protein substrate. Biochim. Biophys. Acta 838,171 - 174.
  • 6. Donella-Deana, A., Grankowski, N., Kudlicki, W., Szyszka, R., Gąsior, E. & Pinna, L.A. (1985) A type I casein kinase from yeast phospho- rylates both serine and threonine residues of casein. Identification of phosphorylation sites. Biochim. Biophys. Acta 829,180 -187.
  • 7. Grankowski, N., Szyszka, R. & Pilecki, M. (1987) Immunological properties and peptide mapping of two type I casein kinases. Acta Biochim. Polon. 34,45 - 49.
  • 8. Rigobello, M.P., Cargnani, G. & Pinna, L.A. (1980) Properties and localization of cAMP independent protein kinase (casein kinase) isolated from yeast mitochondria. FEBS Lett. 121, 225 - 229.
  • 9. Grankowski, N. & Issinger, O.-G. (1990) Subcellular localization of casein kinase I. Biochem. Biophys. Res. Commun. 167,471 - 476.
  • 10. Haas, D.W. & Hagedorn, C.H. (1991) Casein kinase I phosphorylates the 25-kDa mRNA cap-binding protein. Arch. Biochem. Biophys. 284, 84 - 89.
  • 11. Pendergast, A.M. & Traugh, J.A. (1985) Alteration of aminoacyl-tRNA synthetase activities by phosphorylation with casein kinase I. /. Biol. Chem. 260,11769 - 11774.
  • 12. Dahmus, M.E. (1981) Identification of eukaryotic DNA-dependent RNA polymerase. Identification of calf thymus RNA polymerase subunits phosphorylated by two purified protein kinases, correlation with in vivo sites of phosphorylation in HeLa cell RN A polymerase II. /. Biol. Chetn. 256,3332 - 3339.
  • 13. Flotow, H. & Roach, P.J. (1989) Synergistic phosphorylation of rabbit muscle glycogen synthase by cyclic AMP-dependent protein kinase and casein kinase I .J. Biol. Chem. 264,9126 -9128.
  • 14. Szyszka, R., Tchórzewski, M., Dukowski, P., Winiarczyk, S. & Gąsior, E. (1992) The 45 kDa and 27 kDa yeast's protein kinases arc not immunologically related. Acta Biochim. Polon. 39,205-213.
  • 15. Emter, O. & Wolf, D.H. (1984) Vacuoles are not the sole compartments of proteolytic enzymes in yeast. FEBS Lett. 166,321 - 325.
  • 16. Kudlicki, W., Szyszka, R. & Gąsior, E. (1984) A cytoplasmic cyclic nucleotide - independent casein kinase II from Saccharomyces cereztisiar. Biochim. Biophys. Acta 784,102 - 107.
  • 17. Szyszka, R. & Gąsior, E. (1984) Phosphorylation of ribosomal proteins during differentiation of Saccharomyces cerevisiae. Ada Biochim. Polon. 31, 375 - 382.
  • 18. Baumstark, J.S., Laffin, R.R. & Bardavvil, W.A. (1964) A preparative method for the separation of Y-globulin from human serum. Arch. Biochem. Biophys. 108,514-522.
  • 19. Olmsted, J.B. (1981) Affinity purification of antibodies form diazotized paper blots of heterogeneous protein samples. ]. Biol. Chetn. 256,11955 -11957.
  • 20. Harlow, E. & Lane, D. (1988) Cell staining; in Antibodies, pp. 375 - 419, Cold Spring Harbor Laboratory, New York.
  • 21. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227,680 - 685.
  • 22. Yu, I.J., Spector, D.L., Bae, Y.-S. & Marshak, D R. (1991) Immunocytochemical localization of casein kinase II during interphase and mitosis. ¡.Cell Biol. 114,1217-1232.
  • 23. Edelman, A.M., Blumental, D.K. & Krebs, E.G. (1987) Protein serine/threonine kinases. Annu. Rev. Biochem. 56,567 - 613.
  • 24. Fil hol, O., Cochet, C. & Chambaz, E.M. (1990) Cytoplasmic and nuclear distribution of casein kinase II: characterisation of the enzyme uptake by bovine adrenocortical nuclear preparation. Biochemistry 29,9928 - 9936

Typ dokumentu

Bibliografia

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Identyfikator YADDA

bwmeta1.element.agro-article-e695a162-72cc-4321-9bc1-fa785e7a035f
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