DNA and proteins of the nuclear matrix are the main targets of benzo[a]pyrene's action in rat hepatocytes

• Autorzy: Widlak P. , Rzeszowska-Wolny J.
• Języki publikacji: EN

Abstrakty

EN

The binding of [14Clbenzo[a]pyrene (B[aJP) to DNA and proteins in total nuclei and subnuclear fractions of cultured rat hepatocytes was compared. The main targets of BlaJP were non-histone high molecular weight proteins of the nuclear matrix and DNA sequences attached to this structure. Following 24 h exposure to B[alP the amounts of adducts in the nuclear matrix DNA and proteins were twice as high as in total nuclei. After withdrawal of the carcinogen containing medium the level of B[a]P-induced adducts gradually decreased but always remained the highest in the nuclear matrix proteins. Removal of adducts from the nuclear matrix DNA was more efficient than from the other DNA fractions, and 72 h after exposure to the carcinogen the level of DNA adducts in this fraction was similar to that in total nuclei.

Słowa kluczowe

EN

man; hepatocyte; oncology; protein; nuclear matrix; benzo[a]pyrene; DNA; fibroblast; rat

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1993
• Tom: 40
• Numer: 4
• Opis fizyczny: p.559-562,fig.

Twórcy

autor

Widlak P.

• Institute of Oncology, Wybrzeze Armii Krajowej 15, 44-100 Gliwice, Poland

autor

Rzeszowska-Wolny J.

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