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2004 | 09 | 4A |

Tytuł artykułu

Resveratrol protects against peroxynitrite-induced thiol oxidation in blood platelets

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Języki publikacji



The peroxynitrite anion (ONOO·) is a reactive species produced in the reaction between the superoxide anion (O2··) and nitric oxide (·NO). ONOO· is involved in several pathological conditions such as inflammation, arteriosclerosis, and neurodegenerative and cardiovascular disorders. Our earlier results showed that ONOO· inhibits different steps of blood platelet activation and causes the depletion of platelet thiols. In this study, we investigated the effects of resveratrol (3, 4', 5-trihydroxystilbene) and other antioxidants (uric acid and deferoxamine (DFO)) on the level of low molecular thiols such as glutathione, cysteine and cysteinylglycine (in reduced and oxidized form) in blood platelets treated with ONOO·. Our results showed that ONOO· (100 mM, 2 min) induces changes in these thiols (measured by HPLC method); these changes are diminished in the presence of resveratrol. Preincubation of human platelets with resveratrol at a concentration of 100 mM (30 min) has a protective effect against the oxidation of platelet thiols induced by ONOO· or its intermediate. The other tested antioxidants also have a protectory action. In conclusion, we suggest that the resveratrol present in the human diet may partially protect -SH groups from oxidation and may be responsible for redox regulation and control in platelets.

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Opis fizyczny



  • University of Lodz, Banacha 12/16, 90-237 Lodz, Poland


  • 1. Iuliano, L., Colavita, A.R., Leo, R., Practico, D. and Violi, F. Oxygen free radicals and platelet activation. Free Rad. Biol. Med. 22 (1997) 999-1006.
  • 2. Pignatelli, P., Pulcinelli, F.M., Lenti, L., Gazzaniga, P.P. and Violi, F. Hydrogen peroxide is involved in collagen-induced platelet activation. Blood 91 (1998) 484-90.
  • 3. Wachowicz, B., Olas, B., Zbikowska, H.M. and Buczynski, A. Generation of reactive oxygen species in blood platelets. Platelets 13 (2002) 175-182.
  • 4. Brown, A.S., Moro, M.A., Masse, J.M., Cramer, E.M., Radomski, M. and Darley-Usmar, V. Nitric oxide-dependent and independent effects on human platelets treated with peroxynitrite. Cardiovasc. Res. 40 (1998) 380-388.
  • 5. Lufrano, M. and Balazy, M. Interactions of peroxynitrite and other nitrating substances with human platelets: the role of glutathione and peroxynitrite permeability. Biochem. Pharmacol. 65 (2003) 515-523.
  • 6. Nowak, P. and Wachowicz, B. Studies on pig blood platelet responses to peroxynitrite action. Platelets 12 (2001) 376-381.
  • 7. Nowak, P. and Wachowicz, B. The effects of peroxynitrite on pig platelet lipid peroxidation and the secretory process induced by thrombin. Cytobios 106 (2001) 179-187.
  • 8. Essex, D.W. and Li, M. Redox control of platelet aggregation. Biochemistry 42 (2003) 129-136.
  • 9. Nowak, P., Olas, B., Bald, E., Glowacki, R. and Wachowicz, B. Peroxynitrite-induced changes of thiol groups in human blood platelets. Platelets 14 (2003) 375-379.
  • 10. Fremont, L., Belguendouz, L. and Delpal, S. Antioxidant activity of resveratrol and alcohol-free wine polyphenols related to LDL oxidation and polyunsaturated fatty acids. Life Sci. 64 (1999) 2511-2521.
  • 11. Fremont, L. Biological effects of resveratrol. Life Sci. 66 (2000) 663-673.
  • 12. Zbikowska, H.M., Olas, B., Wachowicz, B. and Krajewski, T. Response of blood platelets to resveratrol. Platelets 10 (1999) 247-252.
  • 13. Olas, B., Zbikowska, H.M., Wachowicz, B., Krajewski, T., Buczynski, A. and Magnuszewska, A. Inhibitory effect of resveratrol on free radical generation in blood platelets. Acta Biochim. Polon. 46 (1999) 961-966.
  • 14. Olas, B., Wachowicz, B., Saluk-Juszczak, J., Zielinski, T., Kaca, W. and Buczynski, A. Antioxidant activity of resveratrol in endotoxin-stimulated blood platelets. Cell. Biol. Toxicol. 17 (2001) 117-125.
  • 15. Olas, B. and Wachowicz, B. Resveratrol and vitamin c as antioxidants in blood platelets. Thromb. Res. 106 (2002) 143-148.
  • 16. Olas, B., Wachowicz, B., Szewczuk, J., Saluk-Juszczak, J. and Kaca, W. The effect of resveratrol on the platelet secretory process induced by endotoxin and thrombin. Microbios 105 (2001) 7-13.
  • 17. Olas, B., Wachowicz, B., Saluk-Juszczak, J. and Zielinski, T. Effect of resveratrol, a natural polyphenolic compound, on platelet activation induced by endotoxin or thrombin. Thromb. Res. 107 (2002) 141-145.
  • 18. Pryor, W.A. and Squadrito, G.L. The chemistry of peroxynitrite: a product from the reaction of nitric oxide with superoxide. Am. J. Physiol. 268 (1995) L699-722.
  • 19. Wachowicz, B. and Kustroń, J. Effect of cisplatin on lipid peroxidation in pig blood platelets. Cytobios 70 (1992) 41-47.
  • 20. Walkowiak, B., Michalak, E., Koziołkiewicz, W. and Cierniewski, C.S. Rapid photometric method for estimation of platelet count in blood plasma or platelet suspension. Thromb. Res. 56 (1989) 763-766.
  • 21. Ando, Y. and Steiner, M. Sulfhydryl and disulfide groups of platelet membranes: determination of disulfide groups. Biochim. Biophys. Acta 311 (1973) 26-37.
  • 22. Ando, Y. and Steiner, M. Sulfhydryl and disulfide groups of platelet membranes: determination of sulfhydryl groups. Biochim. Biophys. Acta 311 (1973) 38-44.
  • 23. Glowacki, R., Wójcik, K. and Bald, E. Facile and sensitive method for the determination of mesna in plasma by high-performance liquid chromatography with ultraviolet detection. J. Chromatogr. 914 (2001) 2935.
  • 24. Bald, E., Chwatko, G., Glowacki, R. and Kusmierek, K. Analysis of plasma thiols by high-performance liquid chromatography with ultraviolet detection. J. Chromatogr. 1032 (2004) 109-115.
  • 25. Quijano, C., Alvarez, B., Gatti, R.M., Augusto, O. and Radi, R. Pathways of peroxynitrite oxidation of thiol groups. Biochem. J. 322 (1997) 167-173.
  • 26. Gaston, B. Nitric oxide and thiol groups. Biochim. Biophys. Acta 1411 (1999) 323-333.
  • 27. Minetti, M., Mallozi, C. and Di Stasi, A.M. Peroxynitrite activates kinases of the src family and upregulates tyrosine phosphorylation signalling. Free Rad. Biol. Med. 33 (2002) 744-754.
  • 28. Olas, B., Nowak, P., Kolodziejczyk, J. and Wachowicz, B. The effects of antioxidants on peroxynitrite-induced changes in platelet proteins. Thromb. Res. 113 (2004) 399-406.
  • 29. Bartosz, G. Peroxynitrite: mediator of the toxic action of nitric oxide. Acta Biochim. Pol. 43 (1996) 645-659.
  • 30. Soszynski, M. and Bartosz, G. Effect of peroxynitrite on erythrocytes. Biochim. Biophys. Acta 1291 (1996) 107-114.
  • 31. Moro, M.A., Darley-Usmar, V.M., Goodwin, D.A., Read. N.G., Zamora-Pino, R., Feelisch, M., Radomski, M.W. and Moncada, S. Paradoxical fate and biological action of peroxynitrite on human platelets. Proc. Natl. Acad. Sci. U.S.A. 91 (1994) 6702-6706.
  • 32. Klotz, L.O. and Sies, H. Defenses against peroxynitrite: selenocompounds and flavonoids. Toxicol. Lett. 140 (2003) 125-132.
  • 33. Sies, H. Ebselen, a selenoorganic compound as glutathione peroxidase mimic. Free Rad. Biol. Med. 14 (1993) 313-323.
  • 34. Arteel, G.E., Briviba, K. and Sies, H. Protection against peroxynitrite. FEBS Lett. 445 (1999) 226-230.
  • 35. Practico, D., Pasin, M., Barry, O.P., Ghiselli, A., Sabatino, G., Iuliano, L., Fitzgerald, G.A. and Violi, F. Iron-dependent human platelet activation and hydroxyl radical formation: involvement of protein kinase C. Circulation 99 (1999) 118-124.
  • 36. Bulger, E.M. and Maier, R.V. Antioxidants in critical illness. Arch. Surg. 136 (2001) 1201-1207.
  • 37. Pippard, M.J. and Weatherall, R.V. Deferiprone for thalassaemia. Lancet 35 (2000) 1444-1445.
  • 38. Stojanovic, S., Sprintz, H. and Brede, O. Efficiency and mechanism of the antioxidant action of trans-resveratrol and its analogues in the radical liposome oxidation. Arch. Biochem. Biophys. 391 (2001) 79-89.
  • 39. Arts, M.J.T.J., Haenen, G.R.M.M., Wilms, L.C., Beetstra, S.A.J.N., Heijnen, C.G.M., Voss, H.P. and Bast, A. Interactions between flavonoids and proteins: effect on the total antioxidant capacity. J. Agric. Food Chem. 50 (2002) 1184-1187.
  • 40. Kim, S.R., Park, M.J., Lee, M.K., Sung, S.H., Park, E.J., Kim, J., Kim, S.Y. and Oh, T.H., Markelonis, G.J., Kim, Y.C. Flavonoids of Inula Britannica protect cultured cortical cells from necrotic cell death induced by glutamate. Free Rad. Biol. Med. 32 (2002) 596-604.

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