Wplyw rodzaju tluszczu w diecie na zawartosc wapnia w osoczu krwi i kosci udowej u szczurow doswiadczalnych

• Autorzy: Grajeta H. , Prescha A. , Biernat J.

Warianty tytułu

EN

The effect of dietary fats on calcium content in blood plasma and femoral bone in rats

• Języki publikacji: PL

Abstrakty

PL

Badano wpływ różnego rodzaju tłuszczów pokarmowych na zawartość wapnia w osoczu krwi i kości udowej szczurów doświadczalnych. Stwierdzono, że rodzaj tłuszczu zastosowanego w dietach nie miał istotnego wpływu na zawartość wapnia w osoczu krwi szczurów, natomiast na retencję tego pierwiastka w kości udowej najkorzystniejszy wpływ miała dieta zawierająca olej słonecznikowy i 1% Ca.

EN

The aim of this study was to investigate the effect of dietary fats on calcium content in blood plasma and femoral bone in experimental rats. The study was carried out on female Buffalo rats, which were divided into 6 groups (n=10) and fed diets with 8% butter, sunflower oil or fish oil and with 0,5% or 1% of Ca. At the end of experiment the rats were anesthetized with bioketane and blood was taken by heart puncture and femoral bone were prepared. In the blood hemoglobin content was determined by cyanmethemoglobin method and hematocrite value after centrifugation. In the blood serum calcium content was measured using Biosystems test kit Arsenazo III and alkaline phosphatase activity using Biochemtest test kit. Femoral bone calcium content was determined by atomic emission spectroscopy method and phosphorus content by the method of Scheel. The average calcium content in blood serum of rats was in range from 2,82 to 3,11 mmol/1 and no significant differences were observed between groups of animals. The calcium concentration in blood serum in rats did not depend on kind of dietary fats or on content of Ca in diet. The average calcium content in femoral bone of rats fed diets with butter, sunflower oil and fish oil and 0,5% Ca, was similar in all groups of animals and amounted, respectively: 111,5 m g/g, 110,0 mg/g and 107,5 mg/g. The type of dietary fat in diet did not have significant effect on calcium retention in bones of rats fed diet with 0,5% Ca. In rats fed diets with 1% Ca and different dietary fats, the average calcium content in femoral bone was significantly higher in rats fed diet with sunflower oil (125,1 mg/g) than in rats fed diet with butter (108,2 mg/g) and with fish oil (98,4 mg/g). The kind of dietary fat did not have a significant effect on calcium content in blood serum of tested rats, however, the most beneficial effect on calcium retention in femoral bone, had the diet with sunflower oil and 1% Ca.

Słowa kluczowe

PL

zawartosc wapnia; szczury; kosc udowa; zwierzeta laboratoryjne; tluszcze jadalne; zywienie racjonalne; olej slonecznikowy; wapn; dieta; osocze; zywienie czlowieka

EN

calcium content; rat; femur; laboratory animal; edible fat; rational nutrition; sunflower oil; calcium; diet; plasma; human nutrition

• Wydawca: -
• Czasopismo: Bromatologia i Chemia Toksykologiczna
• Rocznik: 2005
• Tom: 38
• Numer: 2
• Opis fizyczny: s.163-169,tab.,bibliogr.

Twórcy

autor

Grajeta H.

• Akademia Medyczna, pl.Nankiera 1, 50-140 Wroclaw

autor

Prescha A.

autor

Biernat J.