The bacterial artificial chromosome [BAC] library of the narrow-leaved lupin [Lupinus angustifolius L.]

• Autorzy: Kasprzak A , Safar J. , Janda J. , Dolezel J. , Wolko B. , Naganowska B.
• Języki publikacji: EN

Abstrakty

EN

The narrow-leafed lupin possesses valuable traits for environment-friendly agriculture and for the production of unconventional agricultural products. Despite various genetic and environmental studies, the breeding of improved cultivars has been slow due to the limited knowledge of its genomic structure. Further advances in genomics require, among other things, the availability of a genomic DNA library with large inserts. We report here on the construction of the first DNA library cloned in a BAC (bacterial artificial chromosome) vector from diploid Lupinus angustifolius L. cv. Sonet. The high molecular weight DNA used for its preparation was isolated from interphase nuclei that were purified by flow cytometry. The library comprises 55,296 clones and is ordered in 144×384-well microtitre plates. With an average insert size of 100 kb, the library represents six haploid genome equivalents. Thanks to the purification of the nuclei by flow cytometry, contamination with chloroplast DNA and mitochondrial DNA was negligible. The availability of a BAC library opens avenues for the development of a physical contig map and positional gene cloning, as well as for the analysis of the plant’s genome structure and evolution.

Słowa kluczowe

EN

Lupinus angustifolius; plant cultivar; narrow-leaved lupin; Sonet cultivar; genomic structure; bacterial artificial chromosome; genomic DNA library; evolution; plant genome; lupin; diploid; plant breeding

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2006
• Tom: 11
• Numer: 3
• Opis fizyczny: p.396-407,fig.,ref.

Twórcy

autor

Kasprzak A

• Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

autor

Safar J.

autor

Janda J.

autor

Dolezel J.

autor

Wolko B.

autor

Naganowska B.

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