p19 detected in the rat retina and pineal gland is a guanylyl cyclase-activating protein [GCAP]

• Autorzy: Dejda A. , Matczak I. , Gorczyca W.A.
• Języki publikacji: EN

Abstrakty

EN

The Ca2+ -dependent activation of retina-specific guanylyl cyclase (retGC) is medi­ated by guanylyl cyclase-activating proteins (GCAPs). Here we report for the first time detection of a 19 kDa protein (p19) with GCAP properties in extracts of rat retina and pineal gland. Both extracts stimulate synthesis of cGMP in rod outer segment (ROS) membranes at low (30 nM) but not at high (1 uM) concentrations of Ca2+ .AtlowCa2+ , immunoaffinity purified p19 activates guanylyl cyclase(s) in bovine ROS and rat reti­nal membranes. Moreover, p19 is recognized by antibodies against bovine GCAP1 and, similarly to other GCAPs, exhibits a Ca2+ -dependent electrophoretic mobility shift.

Słowa kluczowe

EN

guanylyl cyclase; calcium binding protein; guanylyl cyclase-activating protein; retina; rat; pineal gland

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2002
• Tom: 49
• Numer: 4
• Opis fizyczny: p.899-905,fig.

Twórcy

autor

Dejda A.

• Polish Academy of Sciences, Lodz, Poland

autor

Matczak I.

autor

Gorczyca W.A.

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