Effects of Propionibacterium on the growth and mycotoxin production by some species of Fusarium and Alternaria

• Autorzy: Gwiazdowska D , Czaczyk K. , Filipiak M. , Gwiazdowski R.
• Języki publikacji: EN

Abstrakty

EN

The aim of this research was to study the antifungal properties of Propionibacteria. Three fractions from cultures of Propionibacterium freudenreichii ssp. shermanii 41 and ssp. freudenreichii 111 (i.e. culture containing viable bacteria, cell-free supernatant and bacteriocin preparation) were tested for their ability to inhibit the growth and mycotoxin production of Alternaría altérnala, Fusarium culmorum, Fusarium graminearum and Fusarium verticillioides. The growth of the fungi was monitored during cultivation using a plating method. The concentration of toxins produced was measured by HPLC on the 14th day of culture. Altenuene and tenuazonic acid were determined in cultures of A. altérnala whilst concentration of nivalenol, deoxynivalenol, fumonisin B₁ and zearalenone was measured in Fusarium cultures. The strongest inhibition of growth and toxin production was observed in the presence of cultures containing viable cells and supernatants obtained from Propionibacteria cultures. The bacteriocin extracts generally had a weak fungistatic effect on the growth of A. alternata, F. culmorum and F. graminearum. Despite the fact that growth was slower in the presence of bacteriocin extracts than in control trials, none of the preparations prepared from the Propionibacteria significantly reduced the level of mycotoxin production. The ability of P. freudenreichii ssp. freudenreichii 111 to remove zearalenone from liquid medium was also evaluated. It was shown that both viable and non-viable cells caused a decrease in zearalenone concentration in the medium.

Słowa kluczowe

EN

antifungal property; Propionibacterium; fungi; inhibition; Alternaria; growth; mycotoxin production; Fusarium

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2008
• Tom: 57
• Numer: 3
• Opis fizyczny: p.205-212,fig.,ref.

Twórcy

autor

Gwiazdowska D

• Poznan University of Economics, Al.Niepodleglosci 10, 60-679 Poznan, Poland

autor

Czaczyk K.

autor

Filipiak M.

autor

Gwiazdowski R.

Bibliografia

• Böswald C, G. Engelhardt, H. Vogel and P.R. Wallnöffer. 1995. Metabolism of the Fusarium mycotoxins zearalenone and deoxynivalenol by yeast strains of technological relevance. Nat. Toxins 3: 138-144.
• Bradford M.M. 1976. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
• Côté L.M., A.M. Dahlem, T. Yoshizawa, S.P. Swanson and W.B. Buck. 1986. Excretion of deoxynivalenol and its metabolite in milk, urine and feces of lactating dairy cows. J. Dairy Sci. 69: 2416-2423.
• Czaczyk K., K. Trojanowska and A. Mueller. 2002. Antifungal activity of Bacillus coagulans against Fusarium sp. Acta Microbiol. Polon. 51: 275-283.
• El-Nezami H.S., P.E. Kankaanpää, S. Salminen and J.T. Ahokas. 1998. Ability of dairy strains of lactic acid bacteria to bind common food carcinogens, alfatoxin B₁ Food Chemical Toxicol. 36: 321-326.
• El-Nezami H.S., H. Mykkänen, P.E. Kankaanpää, S. Salminen and J.T. Ahokas. 2000. Ability of Lactobacillus and Propionibacterium strains to remove aflatoxin B1 from the chicken duodenum. J. Food Protect. 63: 549-552.
• El-Nezami H.S., N. Polychronaki, S. Salminen and H. Mykkänen. 2002a. Binding rather than metabolism may explain the interaction of two food-grade Lactobacillus strains with zearalenone and its derivative a-zearalenol. Appl. Environ. Microbiol. 68: 3545-3549.
• El-Nezami H.S., A. Chrevatidis, S. Auriola, S. Salminen and H. Mykkänen. 2002b. Removal of common Fusarium toxins in vitro by strains of Lactobacillus and Propionibacterium. Food Addit. Contamin. 19: 680-686.
• El-Sharkawy S., M. Selim, M. Afifi and F. Halaweish. 1991. Microbial transformation of zearalenone to a zearalenone sulfate. Appl. Environ. Microbiol. 57: 549-552.
• Gourama H. and L.B. Bullerman. 1995. Inhibition of growth and alfatoxin production of Aspergillus flavus by Lactobacillus species. J. Food Protect. 58: 1249-1256.
• Gratz S., H. Mykkänen, A.C. Ouwehand, R. Juvonen, S. Salminen and H. El-Nezami. 2004. Intestinal mucus alters the ability of probiotic bacteria to bind aflatoxin B₁ in vitro. Appl. Environ. Microbiol. 70: 6306-6308.
• Gwiazdowska D. and K. Trojanowska. 2006. Antimicrobial activity and stability of partially purified bacteriocins produced by Propionibacterium freudenreichii ssp. freudenreichii and ssp. shermanii. Le Lait 86: 141-154.
• Haggler W.M., N.R. Towers, C.J. Mirocha, R.M. Eppley and W.L. Bryden. 2001. Zearalenone: mycotoxin or mycoestrogen? In: B.A. Summerell, J.F. Leslie, D. Backhouse, W.L., Bryden and L.W. Burgess (eds), Fusarium. Paul E. Nelson Memorial Symposium. APS Press, St. Paul, Minn., pp. 321-331.
• Higgins C. and F. Brinkhaus. 1999. Efficacy of several organic acids against molds. J. Appl. Poultry Sci. 8: 480-487.
• Jiménez M. and R. Mateo. 1997. Determination of mycotoxins produced by Fusarium isolates from banana fruits by capillary gas chromatography and high performance liquid chromatography. J. Chromatogr. 778: 363-372.
• Karunaratne A, E. Wezenberg and L.B. Bullerman. 1990. Inhibition of mold growth and aflatoxin production by Lactobacillus spp. J. Food Protect. 53: 230-236.
• Kimura N. and S. Hirano. 1988. Inhibitory strains of Bacillus subtilis for growth and aflatoxin production of aflatoxigenic fungi. Agric. Biol. Chem. 52: 1173-1179.
• King A.D. and J.E. Schade. 1984. Alternaría toxins and their importance in food. J. Food Protect. AT: 886-901.
• Kuiper-Goodman T., P.M. Scott, N.P. McEwen, G.A. Lombaert and W. Ng. 1996. Approaches to the risk assessment of fumonisins in corn-based foods in Canada. Adv. Exp. Med. Biol. 392: 369-393.
• Lind H., H. Jonsson and J. Schnürer. 2005. Antifungal effect of dairy Propionibacteria - contribution of organic acids. Int. J. Food Microbiol. 98: 157-165.
• Lyon W. and B. Glatz. 1991. Partial purification and characterization of a bacteriocin produced by Propionibacterium thoenii. Appl. Environ. Microbiol. 57: 701-706.
• Magnüsson J. and J. Schnürer. 2001. Lactobacillus coryniformis subsp. coryniformis strain Si3 produces a broad-spectrum proteinaceous antifungal compound. Appl. Environ. Microbiol. 67: 1-5.
• Marasas W.F.O. 2001. Discovery and occurrence of the fumonisins: a historical perspective. Environ. Health Perspect. 109 (Suppl. 2): 239-243.
• Miescher S., M.P. Stierli, M. Teuber and L. Meile. 2000. Propionicin SM1, a bacteriocin from Propionibacterium jensenii DFL isolation and characterization of the protein and its gene. Syst. Appl. Microbiol. 23: 174-184.
• Motta S. and L.M.V. Soares. 2000. Simultaneous determination of tenuazonic and cyclopiazonic acids in tomato products. Food Chem. 71: 111-116.
• Muller M. and P. Lepom. 1992. The detection of Alternaria mycotoxins in laboratory culture. Zentralblatt fur Mikrobiologie 147: 197-206.
• Munimbazi C. and L.B. Bullerman. 1998. Isolation and partial characterization of antifungal metabolites of Bacillus pumilus. J. Appl. Microbiol. 84: 959-968.
• Osman M.M. 2004. Factors affecting the antifungal properties of Brevibacterium linens. Int. Dairy J. 14: 713-722.
• Paster N., Z. Lecong, M. Menashrov and R. Shapira. 1999. Possible synergistic effect of nisin and propionic acid on the growth of the mycotoxigenic fungi Aspergillus parasiticus, Aspergillus ochraceus, and Fusarium moniliforme. J. Food Protect. 62: 1223-1227.
• Peltonen K., H.S. El-Nezami, S. Salminen and J.T. Ahokas. Binding of aflatoxin B₁ by probiotic bacteria. J. Set, Food Agric. 80: 1941-1945.
• Perkowski J. 1993. Determination of recovering of Fusarium toxins by different methods (in Polish). Roczniki Akademii Rolniczej w Poznaniu 46: 3-15.
• Pitt J.I. and A.D. Hocking. 1999. Fungi and Food Spoilage Second ed. Aspen Publications.
• Sjögren J., J. Magnusson, A. Broberg, J. Schnürer and L. Kenne. 2003. Antifungal 3-hydroxy fatty acids from Lactobacillus plantarum MiLAB 14. Appl. Environ. Microbiol. 69: 7554-7557.
• Stack M.E. and M.J. Prival. 1986. Mutagenicity of the Alternaria metabolites altertoxins I, II and III. Appl. Environ. Microbiol. 52: 718-722.
• Stinson E.E., D.D. Bills, S.F. Osman, J. Siciliano, M.J. Ceponis and E.G. Heisler. 1980. Mycotoxin production by Alternaria species grown on apples, tomatoes and blueberries. J. Agric. Food Chem. 26: 960-963.
• Ström K., J. Sjörgen, A. Broberg and J. Schnürer. 2002. Lactobacillus plantarum MiLAB 393 produces the antifungal cyclic dipeptides cyclo(L-Phe-L-Pro) and cyclo(L-Phe-trans-4-OH-L-Pro) and 3-phenyllactic acid. Appl. Environ. Microbiol. 68: 4322-4327.
• Yekeler H., K. Bitmis, N. Özçelik, M.Z. Doymaz and M. Çalta. 2001. Analysis of toxic effects of Alternaria toxins on esophagus of mice by light and electron microscopy Toxicol. Pathol. 29: 492-497.