Purification and characterization of antibacterial proteins from granular hemocytes of Indian mud crab, Scylla serrata

• Autorzy: Yedery R. D. , Reddy K.V.R.
• Języki publikacji: EN

Abstrakty

EN

Marine invertebrates depend upon antimicrobial peptides (AMPs) as a major component of innate immunity, as they are rapidly synthesized and diffuse upon pathogen invasion. In this study, we report the identification and characterization of a 11 kDa antimicrobial protein, which we name SSAP (for Scylla serrata antimicrobial protein), from granular hemocytes of the mangrove crab S. serrata. The protein is highly similar to scygonadin, a male-specific AMP isolated from the ejaculatory duct of S. serrata. SSAP was isolated using various chromatographic techniques, viz. ion-exchange, ultra filtration and RP-HPLC, and demonstrated antibacterial activity against Gram positive and Gram negative bacteria. Full length mRNA encoding SSAP was amplified using a combination of RT-PCR and RACE. The nucleotide sequence revealed a full-length ORF of 381 bp coding for a preprotein of 126 amino acids comprising a signal peptide of 24 amino acids and a mature protein of 102 amino acids with a predicted mass of 11435 Da and pI of 5.70. Unlike scygonadin, SSAP is expressed in several tissues of both male and female crabs, as evidenced by RT-PCR, Northern and Western blot analyses. The study suggests that SSAP might be an isoform or a variant of scygonadin and might play an important role in regulating the immunity of the crab upon microbial infection.

Słowa kluczowe

EN

isolation; purification; Scylla serrata; Indian mud crab; marine invertebrate; innate immunity; antibacterial protein; granular hemocyte; ejaculatory duct; antimicrobial peptide; crab

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2009
• Tom: 56
• Numer: 1
• Opis fizyczny: p.71-82,fig.,ref.

Twórcy

autor

Yedery R. D.

• National Institute for Research in Reproductive Health, Mumbai, India

autor

Reddy K.V.R.

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