Wystepowanie gatunkow Eimeria u kur na terenie Dolnego Slaska

• Autorzy: Antonik S , Okulewicz A. , Gawel A. , Mazurkiewicz M.

Warianty tytułu

EN

Prevalence of Eimeria species in hens in the Lower Silesia region

• Języki publikacji: PL

Abstrakty

EN

The purpose of the research was to determine the prevalence of particular Eimeria species in hens in the Lower Silesia region and to compare the methods of coccidia species identification. The material for the research came from farms of slaughter chickens and laying hens. The species identification was carried out by the classical method (location of anatomicopathologic changes in the alimentary tract, the morphology and size of oocytes, time of sporulation) and through the isolation of DNA and the amplification chain reaction (PCR). The most frequent species were: E. acervulina, E. tenella, E. maxima and E. necatrix. Over half (69%) of the cases of coccidiosis were caused by at least two species of coccidia, and only 31% of the cases by one species of coccidia. The results of the PCR analysis confirmed the species composition of Eimeria spp. determined by the classical method. For 15 out of 29 isolates, the results of the identification obtained after performing the PCR reaction concur with the results obtained by the classical method. Other tests failed to demonstrate the presence of DNA of the expected coccidian species.

Słowa kluczowe

PL

Dolny Slask; drob; kury; pasozyty zwierzat; pierwotniaki; Eimeria; kokcydia; wystepowanie; czestotliwosc wystepowania; identyfikacja gatunkowa; metody; lancuchowa reakcja polimerazy; metoda klasyczna

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 07
• Opis fizyczny: s.886-888,rys.,tab.,bibliogr.

Twórcy

autor

Antonik S

• Uniwersytet Przyrodniczy we Wroclawiu, pl.Grunwaldzki 45, 50-366 Wroclaw

autor

Okulewicz A.

autor

Gawel A.

autor

Mazurkiewicz M.

Bibliografia

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• 2.Latała A.: Badania nad rodzajem Eimeria u drobiu z terenu woj. opolskiego. Medycyna Wet. 1972, 28, 146-151.
• 3.Mazurkiewicz M., Staszewski S., Zieliński A.: Obserwacje nad występowaniem kokcydioz u kur na terenie Dolnego Śląska. Biul. VI Zjazdu PTNW, Wrocław 1978, s. 633.
• 4.McDougald L. R.: Protozoal infection, [w:] Saif Y. M., Barnes H. J., Glisson J. R., Fadly A. M., McDougald L. R., Swayne D. E. (Eds.): Diseases of Poultry. Iowa State Press, Ames 2003, 974-985.
• 5.Pellerdy L. P.: Coccidia and Coccidiosis. Akademiai Kiado, Budapest 1974, s. 166.
• 6.Schnitzler B. E., Thebo R. L., Mattsson J. G., Tomley F. M., Shirley M. W.: Development of a diagnostic PCR assay the detection discrimination of four pathogenic Eimeria species of the chickens. Avian Pathol. 1998, 27, 490-497.
• 7.Schnitzler B. E., Thebo R. L., Tomley F. M., Uggla A., Shirley M. W.: PCR identification of Eimeria: simplified read-out. Avian Pathol. 1999, 28, 89-93.
• 8.Zhao Xiamin, Duszyński W. D., Loker E. S.: A Simple metod of DNA extraction for Eimeria species. J. Microbiol. Meth. 2001, 44, 131-137.