Porownawcza ocena identyfikacji bakterii z rodzaju Acinetobacter przy uzyciu komercyjnego systemu API 20NE oraz technika PCR i RFLP

• Autorzy: Wydmuch Z. , Kepa M. , Wojtyczka R. , Idzik D. , Pacha J.
• Języki publikacji: PL

Abstrakty

PL

Zidentyfikowano bakterie z rodzaju Acinetobacter systemem API 20NE oraz techniką PCR/RFLP. Wykryty gen recA poddano trawieniu endonukleazami restrykcyjnymi Mbol i Hinfl. Restrykcyjna analiza umożliwiła określenie gatunków genowych wszystkich badanych szczepów.

EN

A study was carried out for identification of 50 Acinetobacter strains isolated from various clinical materials. Using classic methods the following species were identifies: Acinetobacter sp. (68%), Acinetobacter baumanii (24%) and Acinetobacter lwofä (8%). In all strains the recA gene was found of 435-500 pz size which confirms their belonging to that genus. Amplification products were digested with restriction enzymes Mbol and Hinfl (RFLP) and their detection was carried out on agarose gel by electrophoresis methods, owing to that the arrangement of gene fragment characteristic of each strain was obtained. After careful analysis restriction patterns were obtained corresponding to the following genome species: Acinetobacter baumanii (60%), Acinetobacter sp. 3 (28%) and Acinetobacter Iwoffii (12%). The methods of molecular biology made possible a more precise classification of the studied strains according to species. Certain strains determined as Acinetobacter sp. by the API 20NE system were found to be Acinetobacter baumanii, Acinetobacter sp. 3 or Acinetobacter Iwofii when determined by the PCR/RFLP method.

Słowa kluczowe

PL

metoda RFLP; lancuchowa reakcja polimerazy; analiza porownawcza; bakterie; Acinetobacter; endonukleazy; system API 20NE; identyfikacja

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2000
• Tom: 52
• Numer: 3
• Opis fizyczny: s.267-273,fot.,bibliogr.

Twórcy

autor

Wydmuch Z.

• Akademia Medyczna, 41-200 Sosnowiec, ul.Jagiellonska 4

autor

Kepa M.

autor

Wojtyczka R.

autor

Idzik D.

autor

Pacha J.

Bibliografia

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