Effect of dietary supplemental black cumin seeds on antioxidant activity in broilers

• Autorzy: Guler T , Ertas O.N. , Kizil M. , Dalkilic B. , Ciftci M.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to investigate the potential antioxidant activity of black cumin seeds as a dietary supplement. Three hundred 3-day-old broiler chicks were divided randomly into five treatment groups (control, and black cumin at four levels) of 60 birds each. Black cumin seeds at 0.5%, 1%, 2% or 3% were added to the basal diet and their effects determined on malondialdehyde (MDA) and vitamin E levels in the serum, breast muscles, liver and heart muscles in the 42-day old broilers. When the diet was supplemented with 2% and 3% black cumin seed MDA concentration in serum (p < 0.05), breast muscle (p < 0.01), liver (p < 0.05) and heart muscle (p < 0.05) was significantly more reduced compared to birds fed 1% and 0.5% black cumin seeds and control diet. Supplementing the diet with black cumin seeds did not significantly affect vitamin E levels in serum and tissues. These results suggest that black cumin seeds could be considered as a natural potential antioxidant promoter for poultry, and showed the best responses at 2% and 3% level of inclusion.

Słowa kluczowe

EN

black cumin; seed; malondialdehyde; antioxidant activity; animal feeding; diet supplementation; broiler; poultry; diet; chemical composition; dietary effect

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2007
• Tom: 63
• Numer: 09
• Opis fizyczny: p.1060-1063,ref.

Twórcy

autor

Guler T

• University of Firat, 23119 Elazig, Turkey

autor

Ertas O.N.

autor

Kizil M.

autor

Dalkilic B.

autor

Ciftci M.

Bibliografia

• 1.A.O.A.C.: Official Methods of Analysis. Association of Official Analytical Chemists. Arlington, Virginia 1990.
• 2.Al-Gaby A. M.: Amino acid composition and biological effects of supplementing broad bean and corn proteins with Nigella sativa (Black cumin) cake protein. Nahrung. 1998, 42, 290-294.
• 3.Al-Ghamdi M. S.: The anti-inflammatory analgesic and antipyretic activity of Nigella sativa. J. Ethnopharmacol. 2001, 76, 45-48.
• 4.Al-Hader A., Aqel M., Hasan Z.: Hypoglycemic effects of the volatile oil of Nigella sativa. Int. J. Pharmacognosy. 1993, 31, 96-100.
• 5.Badary O. A., Al-Shabanah O. A., Nagi M. N., Al-Bekairi A. M., El- -Mazar M. M.: Acute and subchronic toxicity of thymoquinone in mice. Drug Develop. Res. 1998, 44, 56-61.
• 6.Badary O. A., Taha R. A., Gamal el-Din A. M., Abdel-Wahab M. H.: Thymoquinone is a potent superoxide anion scavenger. Drug Chem. Toxicol. 2003, 26, 87-98.
• 7.Burtis M., Bucar F.: Antioxidant activity of Nigella sativa essential oil. Phytothrapy Res. 2000, 14, 323-328.
• 8.Cheeseman K. H.: Mechanism and effects of lipid peroxidation. Molec. Asp. Med. 1993, 14, 191-197.
• 9.Chowdhury A. K. A., Islam A., Rashid A., Ferdous A.: Therapeutic potential of the volatile oil of Nigella sativa seeds in monkey model with experimental shigellosis. Phytotherapy Res. 1998, 12, 361-363.
• 10.Crampton E. W., Maynard L. A.: The Relation of cellulose and lignin content to nutritive value of animal feeds. J. Nutr. 1983, 15, 383-395.
• 11.Daba M. H., Abdel-Rahman M. S.: Hepatoprotective activity of thymoquinone in isolated rat hepatocytes. Toxicoll. Lett. 1998, 95, 23-29.
• 12.Farah N., Benghuzzi H., Tucci M., Cason Z.: The effects of isolated antioxidants from black seed on the cellular metabolism of A549 cells. Biomed. Sci. Instrum. 2005, 41, 211-216.
• 13.Ferdous A. J., Islam S. N., Ahsan M., Hasan C. M., Ahmad Z. U.: In vitro antibacterial activity of the volatile oil of Nigella sativa seeds against multiple drug-resistant isolates of Shigella species and isolates of Vibrio cholerae and Escherichia coli. Phytotherpy Res. 1992, 6, 137-140.
• 14.Ghosheh O. A., Houdi A. A., Crooks P. A.: High performance liquid chromatography analysis of the pharmacologically active quinines and related compounds in the oil of the black seed (Nigella sativa). J. Pharm. Biomed. Ana. 1999, 19, 757-762.
• 15.Gilani A. H., Aziz N., Khurram I. M., Chaudhary K. S., Iqbal A.: Bronchodilator, spasmolytic and calcium antagonist activities of Nigella sativa seeds (Kalonji): a traditional herbal product with multiple medicinal uses. J. Pak. Med. Assoc. 2001, 51, 115-120.
• 16.Gilani A. H., Jabeen Q., Khan M. A. U.: A Review of Medicinal Uses and Pharmacological Activities of Nigella sativa. Pakistan J. Biol. Sci. 2004, 7, 441-451.
• 17.Guler T., Dalkýlýç B., Ertas O. N., Çiftçi M.: The Effect of Dietary Black Cumin Seeds (Nigella Sativa L.) on the Performance of Broilers. Asian-Aust. J. Anim. Sci. 2006, 19, 425-430.
• 18.Houghton P. J., Zarka R., de las Heras B., Hoult J. R.: Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med. 1995, 61, 33-36.
• 19.Ilhan A., Gurel A., Armutcu F., Kamisli S., Iraz M.: Antiepileptogenic and antioxidant effects of Nigella sativa oil against pentylenetetrazol-induced kindling in mice. Neuropharmacology 2005, 49, 456-464.
• 20.Kapoor L. D.: Handbook of Ayurvedic Medicinal Plants. CRC Press, Inc. Boca Raton, Florida, USA 1990.
• 21.Katsanidis E., Addis P. B.: Novel HPLC analysis of tocopherols, and cholesterol in tissue. Free Radic. Biol. and Med. 1999, 27, 1137-1140.
• 22.Khan M., Shaila Jabbar T. H., Choudhuri M. S. K., Ghafur M. A.: Analgesic and antiinflammatory activity of Nigella sativa Linn. Hamdard Medicus. 1999, 42, 22-29.
• 23.Khan N., Sultana S.: Inhibition of two stage renal carsinogenesis, oxidative damage and hyperproliferative response by Nigella sativa. European J. Cancer Prevent. 2005, 14, 159-168.
• 24.Mabrouk G. M., Moselhy S. S., Zohny S. F., Ali E. M., Helal T. E., Amin A. A., Khalifa A. A.: Inhibition of methylnitrosourea (MNU) induced oxidative stress and carcinogenesis by orally administered bee honey and Nigella grains in Sprague Dawely rats. J Exp. Clin. Cancer Res. 2002, 21, 341-346.
• 25.Mahmoud M. R., El-Abhar H. S., Saleh S.: The effect of Nigella sativa oil against the liver damage induced by Schistosoma mansoni infection in mice. J. Ethnopharmacology. 2002, 79, 1-11.
• 26.Mahmoud N. N., Mansour A. M.: Protective effect of thymoquinone against doxorubicin-induced cardiotoxicity in rats: A possible mechanism of protection. Pharmacological Res. 2000, 41, 283-289.
• 27.Mansour M. A., Ginawi O. T., El-Hadiyah T., El-Khatib A. S., Al-Shabanah O. A., Al-Sawaf H. A.: Effects of volatile oil constituents of Nigella sativa on carbon tetrachloride-induced hepatotoxicity in mice: evidence for antioxidant effects of thymoquinone. Res. Commun. Mol. Pathol. Pharmacol. 2001, 110, 239-251.
• 28.Meral I., Yener Z., Kahraman T., Mert N.: Effect of Nigella sativa on glucose concentration, lipid peroxidation, anti-oxidant defence system and liver damage in experimentally-induced diabetic rabbits. J. Vet. Med. A. 2001, 48, 593-599.
• 29.Nagi M. K., Alam K., Badary O. A., Al-Shabanah O. A., Al-Sawaf H. A., Al-Bekairi A. M.: Thymoquinone protects against carbon tetrachloride hepatotoxicity in mice via an antioxidant mechanism. Biochem. Mol. Biol. Int. 1999, 47, 153-159.
• 30.National Research Council (NRC): Nutrient Requirements of Poultry. National Academy Press, Washington, DC 1994.
• 31.Ozogurlu F., Sahin S., Idýz N., Akyol O., Ilhan A., Yigitoglu R., Isýk B.: The effect of Nigella sativa oil against experimental allergic encephalomyelitis via nitric oxide and other oxidative stress parameters. Cell Mol. Biol. 2005, 51, 337-342.
• 32.Paradis V., Kollinger M., Fabre M., Holstage A., Poynard T., Bedossa P.: In situ detection of lipid peroxidation by-products in chronic liver diseases. Hepatology. 1997, 26, 135-143.
• 33.Placer A. Z., Linda L. C., Johnson B.: Estimation of product of lipid peroxidation (malonyldialdehyde) in biochemical systems. Anal. Biochem. 1966, 16, 359-364.
• 34.Sevanian A., McLeod L.: Formation of Biological Reactivity of Lipid Peroxidation Products. Taylor and Francis, Washington: DC 1997, 47-70.
• 35.Shoieb A. M., Elgayyar M., Dudrick P. S., Bell K. L., Tithof P. K.: In vitro inhibition of growth and induction of apoptosis in cancer cell lines by thymoquinone. Int. J. Oncol. 2003, 22, 107-113.