Infectious transcripts from cloned cDNA of potato leafroll luteovirus

• Autorzy: Sadowy E. , Pluta K. , Gronenborn B. , Hulanicka D.
• Języki publikacji: EN

Abstrakty

EN

Infectious transcripts play a key role in the research on plant viruses at the molecular level. A number of cDNA clones covering the whole genome of the Polish isolate of potato leafroll virus were constructed. Four overlapping clones were selected and assembled using restriction sites. The full copy was positioned between T7 RNA polymerase promoter and unique ScaI site. The full-length capped transcripts of the sequence of the viral genome synthesised in vitro were able to replicate in protoplasts and to produce the viral coat protein

Słowa kluczowe

EN

molecular level; infectious transcript; cDNA clone; virus; luteovirus; potato leafroll virus; RNA transcription; viral replication; plant virus; in vitro

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 2
• Opis fizyczny: p.611-619,fig.

Twórcy

autor

Sadowy E.

• Polish Academy of Sciences, A.Pawinskiego 5A, 02-106 Warsaw, Poland

autor

Pluta K.

autor

Gronenborn B.

autor

Hulanicka D.

Bibliografia

• 1. Boyer, J.-Ch. & Haenni, A.-L. (1994). Infec­tious transcripts and cDNA clones of RNA vi­ruses. A review. Virology 198, 415-426.
• 2. Sadowy, E., Zagórski, Wł. & Hulanicka, D. (1996) Infekcyjne klony cDNA roślinnych wi­rusów. A review. Biotechnologia 34, 60-68.
• 3. Matthews, R.E.F. (1991) Plant Virology; pp. 226-229, Academic Press, New York.
• 4. Rochow, W.F. & Duffus, J.E. (1981). Luteovi- ruses and yellows diseases; in: Handbook of Plant Virus Infections and Comparative Diagno­sis (Kurstak, E., ed.) pp. 147-170, Els- vier/NorthHolland, Amsterdam.
• 5. Mayo, M.A., Barker. H., Robinson, DJ., Ta- mada, T. & Harrison, B.D. (1982). Evidence that potato leafroil virus RNA is positive- stranded, is linked to a small protein and does not contain polyadenylate. J. Gen. Virol. 59, 163-167.
• 6. Harrison, B.D. (1984) Potato Leafroil Virus. CM1/AAB Description of Plant Viruses, No. 291.
• 7. Van den Heuvel, J.F.J.M., Verbeek, M. & van der Wilk, F. (1994). Endosymbiotic bacteria associated with circulative transmission of po­tato leafroil virus by Myzus persicae. J. Gen. Vi­rol 75, 2559-2565.
• 8. Mayo, M.A., Robinson, DJ., Jolly, C.A. & Hy- man, L. (1989) Nucleotide sequence of potato leafroil luteovirus RNA. J. Gen. Virol. 70, 1037-1051.
• 9. Van der Wilk, F., Huisman, M.J., Cornelissen, B.J., Huttinga, H. & Goldbach, R. (1989). Nu­cleotide sequence and organization of potato leafroil virus genomic RNA. FEBS Lett. 245, 51-56.
• 10. Keese, P., Martin, R.R., Kawchuk, L.M., Wa- terhouse, P.M. & Gerlach, W.L. (1990) Nucleo­tide sequences of an Australian and a Cana­dian isolate of potato leafroil luteovirus and their relationships with two European iso­lates. J. Gen. Virol. 71, 719-724.
• 11. Palucha, A., Sadowy, E., Kujawa, A., Juszczuk, M., Zagörski, W. & Hulanicka, D. (1994). Nu­cleotide sequence of RNA of a Polish isolate of potato leafroil luteovirus. Acta Biochim. Polon. 41, 405-414.
• 12. Tacke, E., Prüfer, D., Salamini, F. & Rohde, W. (1990) Characterisation of a potato leafroil luteovirus subgenomic RNA: Differential ex­pression by internal translation initiation and UAG suppression. J. Gen. Virol 71, 2265- 2272.
• 13. Mayo, M.A. & Ziegler-Graff, V. (1996). Mo­lecular biology of luteoviruses. Adv. Virus Res. 46, 416-460.
• 14. Leiser, R.-M., Ziegler-Graff, V., Reutenauer, A., Herrbach, E., Lemaire, O., Guilley, H., Rirhards, K. & Jonard, G. (1992) Agroinfcc tion as an alternative to insects for infecting plants with beet western yellows luteovirus. Proc. Natl Acad. Sei. U.S.A. 89, 9136-9140.
• 15. Prüfer, D., Wipf-Schibel, C.f Richards, K., Guiley, H., Lecoq, H. & Jonard, G. (1995) Syn­thesis of a full-length infectious cDNA clone of cucurbit aphid-borne yellows virus and its use in gene exchange experiments with structural proteins from other luteoviruses. Virology 214, 150-158.
• 16. Prüfer, D., Schmitz, J., Tacke, E., Kull, B. & Rohde, W. (1997) In vivo expression of a full- length cDNA copy of potato leafroll virus (PLRV) in protoplasts and transgenic plants. Mol. Gen. Genet. 253, 609-614.
• 17. Tamada, T. & Harrison, B.D. (1980) Factors affecting the detection of potato leafroll virus in potato foliage by enzyme-linked immuno­sorbent assay. Ann. AppL Biol 95, 209-219.
• 18. Chomczynski, P. & Sacchi, N. (1987) Single- step method of RNA isolation by acid guanid- inium thiocyanate-phenol-chloroform extrac­tion. Anal Bioehem. 162, 156-159.
• 19. Maniatis, T., Fritsch, E.F. & Sambrook, J. (1989) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory.
• 20. Heusterspreute, M., Vinch Ha Thi, Emery, S., Tournis-Gamble, S., Kennedy, N. & Davison, J. (1987) Vectors with restriction site banks IV. pJRD184, a 3793-bp plasmid vector with 49 unique sites. Gene 53. 299-300.
• 21. Laemmli, U.K. (1970) Cleavage of otructural proteins during assembly of the head of bacte­riophage T4. Nature (London) 227,680-685.
• 22. Guerche, P., Bellini, C.. Le Moullec, J.-M. & Caboche, M. (1987) Use of a transient expres­sion assay for the optimisation of direct gene transfer into tobacco mesophyl protoplasts by electroporation. Biochimie 69. 621-628.
• 23. Weiland, J.J. & Dreher, T.W. (1989) Infec­tious TYMV RNA from cloned cDNA: Effects in vitro and in vivo of point substitutions in the initiation codons of two extensively overlap­ping ORFs. Nucleic Acids Res. 17,4675-4687.
• 24. Ausubel, F.M. & Brent, R. (1987). Current Pro­tocols in Molecular Biology. Greene Publish­ing Associates, Brooklyn NY.
• 25. Eggen, R., Verver, J., Wellink, J., DeJong, A., Goldbach, R. & van Kämmen, A. (1989) Im­provements of the infectivity of in vitro tran­scripts from cloned cowpea mosaic virus cDNA: Impact of terminal nucleotide se­quences. Virology 173. 445-455.
• 26. Guilley, H., Dudley, R.K., Jonard, G., Balazs, E. & Richards, K.E. (1982) Transcription of cauliflower mosaic virus DNA: Detection of promoter sequences and characterisation of the transcripts. Cell 30, 763-773.
• 27. Brault, V., van den Heuvel, J.M.F.M., Ver- beek, M., Ziegler-Graff, V., Reutenauer, A., Herrbach, E., Garaud, J.-C., Guilley, H., Rich­ards. K. & Jonard, G. (1995) Aphid transmis­sion of beet western yellows luteovirus re­quires the minor capsid read-through protein P74. EM BO J. 14, 650-659.
• 28. Bruyere, A., Brault, V., Ziegler-Graff. V., Si­monis, M.-T., van den Heuvel, J.F.J.M., Rich­ards, K., Guilley, H., Jonard, G. & Herrbach, E. (1997) Effects of mutations in the beet west­ern yellows virus readthrough protein on its expression and packaging, and on virus accu­mulation, symptoms, and aphid transmission. Virology 230. 323-334.
• 29. Reutenauer, A., Ziegler-Graff, V., Lot, H., Scheidecker, D., Guilley, H., Richards, K. & Jonard, G. (1993) Identification of beet west em yellows luteovirus genes implicated in vi­ral replication and particle morphogenesis. Vi­rology 195, 692-699.
• 30. Ziegler-Graff, V., Brault, V., Mutterer, J.D., Si­monis, M.-T., Herrbach, E., Guilley, H., Rich­ards, K.E. & Jonard. G. (1996) The coat pro­tein of beet western yellows luteovirus is es-sential for systemic infection but the viral gene product* P29 and P19 are dispensable for systemic infection and aphid transmission. MPM1 9, 501-510.
• 31. Veidt, I., Bouzoubaa, S.E., Leiser, R.-M., Ziegler-Graff, V., Guilley, H., Richards, K. & Jonard, G. (1991) Synthesis of full-length tran­scripts of beet western yellows virus RNA: Messenger properties and biological activity in protoplasts Virology 186, 192-200.
• 32. Young, M.J., Kelly, P.J., Larkin, P.J., Water- house, P.M. & Gerlach, W.L. (1990) Infectious in vitro transcripts from a cloned cDNA of bar­ley yellow dwarf virus. Virology 180.372-379.
• 33. Bahner, I., Lamb, J., Mayo, M.A. & Hay, R.T. (1990) Expression of the genome of potato lea­froll virus: Readthrough of the coat protein termination codon in vivo. J. Gen. Virol. 71, 2251-2256.