Induction of a protective immune response against Chlamydophila psittaci in SPF chicken following vaccination with Omp-1 DNA and CpG oligonucleotides as an adjuvant

• Autorzy: He C , Li S. , Yang L. , He G. , Liu W. , Yao B.
• Języki publikacji: EN

Abstrakty

EN

In the present study we determined whether CpG motifs could be used as an immune adjuvant for the Omp-1 DNA vaccine against Chlamydophila psittaci, based on the major outer membrane protein (MOMP), to induce the protection in specific pathogen free chickens against an intranasal challenge. Six sequences of phosphorothioate-CpG oligodeoxynucleotides (CpG ODNs) with strong immunostimulatory activity were designed to be examined. The CpG ODN with three GTCGTT repeats significantly promoted splenic lymphocyte proliferation and increased macrophage nitric oxide in a in vitro study. Birds vaccinated with CpG ODN and pcDNA3.1:MOMP induced moderate antibody response and higher lymphoproliferative stimulation while chickens primed-boosted with DNA alone did not. Furthermore, DNA vaccine plus CpG ODN accelerated chlamydial clearance in the spleen and lungs, and decreased inflammatory cellular infiltration. All above evidences show that CpG ODN can be used as an effective adjuvant with a DNA vaccine and this immunisation protocol resulted in an enhanced clearance of Chlamydophila psittaci.

Słowa kluczowe

EN

protective immune response; avian chlamydiosis; China; major outer membrane protein; animal disease; chlamydiosis; vaccination; prevalence; Omp-1 DNA vaccine; veterinary medicine; CpG oligonucleotide; protection; Chlamydophila psittaci; immunization; bacteria; chicken; immune response

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2007
• Tom: 51
• Numer: 3
• Opis fizyczny: p.351-356,fig.,ref.

Twórcy

autor

He C

• College of Veterinary Medicine, China Agricultural University, Beijing 100094, China

autor

Li S.

autor

Yang L.

autor

He G.

autor

Liu W.

autor

Yao B.

Bibliografia

• 1. Andersen A.A.: Avian chlamydiosis. In: OIE Standard Commission, Manual of Standards for Diagnostic Tests and Vaccines. 4th ed. Paris, Office International des Epizooties, 2000, pp.679-690.
• 2. Berry L.J., Hickey D.K., Skelding K.A., Bao S., Rendina A.M., Hansbro P.M., Gockel C.M., Beagley K.W.: Transcutaneous immunization with combined cholera toxin and CpG adjuvant protects against Chlamydia muridartim genital tract infection. Infect Immun 2004, 72, 1019-1028.
• 3. Cao J., Yang Q., Yang L., He. C.: Investigation of avian Chlamydophila psittaci in Beijing and 6 provinces around the capital. Vet Sci China 2006, 36, 931-937.
• 4. Dalloul R. A., Lillehoj H. S., Okamura M., Xie H., Min W., Ding X., Hecker R.A.: In vivo effects of CpG oligodeoxynucleotide on Eimeria infection in chickens. Avian Dis 2004, 48, 783-790.
• 5. Gomis S., Babiuk L., Allan B., Willson P., Waters E., Ambrose N., Hecker R., Potter A.: Protection of neonatal chicks against a lethal challenge of Escherichia coli using DNA containing cytosine-phosphodiester-guanine motifs. Avian Dis 2004, 48, 813-822.
• 6. Green L., Wagner D., Glogowski J., Skipper P., Wishnok J., Tannenbaum S.: Analysis of nitrite and [¹⁵N] nitrate in biological fluids. Analyt Biochem 1982, 126, 131-138.
• 7. Harmann G., Krieg A .M.: Mechanism and function of a newly identified CpG DNA motif in human primary B cells. J Immunol 2000, 164, 944-953.
• 8. He H., Crippen T.L., Farnell M.B., Kogut M.H.: Identification of CpG oligodeoxynucleotide motifs that stimulate nitric oxide and cytokine production in avian macrophage and peripheral blood mononuclear cells. Dev Comp Immunol 2003, 27, 621-627.
• 9. Loots K., Loock M.V., Vanrompay D., Goddeeris B.M.: CpG motifs as adjuvant in DNA vaccination against Chlamydophila psittaci in turkeys. Vaccine 2006, 24, 4598-4601.
• 10. Jin H.L., Kang Y.M., Zheng G.X., Xie Q., Xiao H., Zhang X.Y., Yu Y., Zhu K., Zhao G., Zhang F., Chen A., Wang B.: Induction of active immune suppression by co-immunization with DNA- and protein-based vaccines. Virology 2005, 337, 183-191.
• 11. Mosmann T.: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Meth 1983, 65, 55-63.
• 12. Oshop G.L., Elankumaran S., Heckert R.A.: DNA vaccination in the avian. Vet Immunol Immunopathol 2002, 89, 1-12.
• 13. Pontarollo R.A., Rankin R., Babiuk L.A., Godson D. L., Grienel P.J., Hecker R., Krieg A.M., Hurk V.D.: Monocytes are required for optimum in vitro stimulation of bovine peripheral blood mononuclear cells by nonmethylated CpG motifs. Vet Immunol Immunopathol 2002, 84, 43-59.
• 14. Rankin R., Pontarollo R., Gomis S., Gomis S.M., Karvonen B., Wilson P., Loehr B.I.: CpG-containing oligodeoxynucleotides augment and switch the immune responses of cattle to bovine herpesvirus-1 glycoprotein D. Vaccine 2002, 20, 3014-3022.
• 15. Vanrompay D., Cox E., Mast J, Goddeeris B., Volchaert G..: Turkeys are protected from infection with Chlamydia psittaci by plasmid DNA vaccination against the major outer membrane protein. Clin Exp Immunol 1999, 118, 49-55.
• 16. Vanrompay D., Van Nerom A., Ducatelle R., Haesebrouk F.: High level expression of Chlamydia psittaci major outer membrane protein in COS cells and in skeletal muscles of turkeys. Infect Immun 1998, 66, 5494-5500.
• 17. Verminnen K., Loock M.V., Cox F., Goddeeris B.M., Vanrompay D.: Protection of turkeys against Chlamydophila psittaci challenge by DNA and rMOMP vaccination and evaluation of the immunomodulating effect of 1 alpha,25-dihydroxyvitamin D(3). Vaccine 2005, 23, 4509-4620.
• 18. Xie H., Raybourne R.B., Babu U.S., Lillehoj H.S., Heckert R.A.: CpG-induced immunomodulation and intracellular bacterial killing in a chicken macrophage cell line. Dev Comp Immunol 2003, 27, 823-834.
• 19. Yang Q., Cheng H., Ming L., Li Y.: Cloning and prokaryotic expression of omp-1 of swine Chlamydophila abortus CP12. J China Agri Univ 2005, 10, 56-59.
• 20. Zhang L., Tian X., Zhou F.: Vaccination with Newcastle disease vaccine and CpG oligodeoxynucleotides induces specific immunity and protection against Newcastle disease virus in SPF chickens. Vet Immunol Immunopathol 2007, 115, 216-222.