Effect of cadmium on collagen content and solubility in rat bone

• Autorzy: Galicka A. , Brzoska M.M. , Sredzinska K. , Gindzienski A.
• Języki publikacji: EN

Abstrakty

EN

The toxic action of cadmium in the bone tissue is known, but its mechanisms are still unexplained. We examined whether Cd influences collagen content and its solu­bility in the femoral bone of three-week-old female rats exposed to 5 or 50 mg Cd/l in drinking water. Non-cross linked collagen was extracted with 0.5 M acetic acid, and two acid-insoluble collagen fractions were extracted with pepsin and 4.0 M guanidine hydrochloride, respectively. SDS/PAGE showed the presence of two colla­gen types, I and V, in all three extracted fractions. Exposure of rats to Cd for 6 months increased the amount of acid-soluble collagens type I and V and decreased the level of acid-insoluble collagens. The amount of total collagen extracted from the bones of rats exposed to 50 mg Cd/l was reduced by about 14% as compared to con­trol and those intoxicated with 5 mg Cd/l. The solubility of type I bone collagen (de­termined as the percentage of acetic-soluble fraction of total collagen) was increased 2.9- and 3.0-fold in rats intoxicated with 5 and 50 mg Cd/l, respectively. Similarly, the solubility of type V collagen was increased 2.3- and 2.7-fold, respectively. Our re­sults indicate that Cd treatment affects bone collagen by decreasing its content and increasing its solubility.

Słowa kluczowe

EN

collagen content; bone; cadmium; solubility; rat

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2004
• Tom: 51
• Numer: 3
• Opis fizyczny: p.825-829,fig.,ref.

Twórcy

autor

Galicka A.

• Medical University of Bialystok, 15-230 Bialystok 8, Poland

autor

Brzoska M.M.

autor

Sredzinska K.

autor

Gindzienski A.

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